FastQCFastQC Report
Sat 31 Dec 2022
SRR5846060.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846060.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80991
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCGTCT8341.0297440456347002No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGC7780.9606005605561112No Hit
ACACATGGGCTTGCATTATTTGTGTGCTTTATGCTTTTTAGAAAATTTTTCC4700.5803113926238718No Hit
GGGCACGGGATTTCTGACCCATTTATTGGTATACTGTCACTTCCCTTTTTTT2380.29385981158400315No Hit
TTACTTGGGAAGAATCAGATTGGAGATAAAGAAAAGCTGGGAGGTAAACTTT1530.18890987887543062No Hit
CTTATACACATCTCCGAGCCCACGAGACGGCAGACTATCTCGTATGCCTGTC1450.1790322381499179No Hit
TCTCGGGGGCCGGCTGCAGGGATGGCGGGGAGGGGTAAGCTCATCGCAGTGA1430.17656282796853973No Hit
GGGCTGGGGAGTTAAAGCCATCTACCGAGAAGTGTTAGTTCAGTTGGAAGTG1360.1679198923337161No Hit
CTAGGCGGGATAAATCAAGCAGTAGTAAGATAGTGAGCATTCATACAAAAAT1340.1654504821523379No Hit
TCAGCTGGGATTAGGCAAGAAACTGATTCACTGCAAAACTGGAAACCAAGGA1290.15927695669889247No Hit
TCTTCGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC1290.15927695669889247No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACT1260.1555728414268252No Hit
AATGGTGGGGAAAAAAATAGCTGGGCATGGTGGTACACGTCTGTAGTCCCAG1230.15186872615475794No Hit
TGCCGAGGGACCAGCAATCTGTAACATCCCTAGAAAATTGTCTAGAACACAG1200.14816461088269067No Hit
TACAAGCGGGGGCTAGTGGAAATCCTGAATTGCTTCCTTTCTGTGATCACTG1200.14816461088269067No Hit
TTGCTGAGGGGTTGGTGGGACTGTAAACTAGTTCAACCATTGTGGAAGTCAG1180.1456952007013125No Hit
TTGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.1432257905199343No Hit
TAAAAAGGGTATACACTCCCACCAACAATGTATTAGCATTCTCTTATAGACT1150.1419910854292452No Hit
GCGTCAGGGGTGTCTCCCTAGCAAGATGGACACTGTGCAGGAAGGAAATGGG1150.1419910854292452No Hit
GTGGCCGGGCAAAGGAACTTTCTATTGATCTTGAATGCAACACTGTTTCTCA1140.14075638033855614No Hit
AAGACGGGGCAGCCTCATGAGTAGCTGGGATTGCAGGCACGTGCCACCGCGC1140.14075638033855614No Hit
AATCAAGGGAAGATCTTTATAAGGAGAACTACAAAACATTGATGAAAGAAAT1130.13952167524786704No Hit
TAAATAGGGAAGCCTACGTTTTCACACTTCTAGTAAGCCTCTACCTGCACGA1100.13581755997579978No Hit
CTCTTAGGGATAGAAACAAAGCAATTCCAGTAAGATATATAAAATATGTGCA1100.13581755997579978No Hit
TGGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1090.1345828548851107No Hit
GTCGAAGGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAAC1070.1321134447037325No Hit
GACATGGGGATCCTGAGTCCTCAACAATTAAATAACTTGGACAAATTCACTG1070.1321134447037325No Hit
TACGTGGGGGAAGAAGCACCAGGCCATGGAGGCCATGCTGCTGTACCTGGCC1070.1321134447037325No Hit
GTATTTGGGTGACTCCTTCAAGTGTTCAGTAGTACAATAGGTAATCAATAAT1070.1321134447037325No Hit
GCCATAGGGACTAATAAGGAGGTTCCAAGACTTCCCTTGGTTTAATGAGAAC1060.13087873961304342No Hit
GGAACTGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1060.13087873961304342No Hit
AGTCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.12964403452235435No Hit
AGCTAAGGGTGAAGACCTTGGTATTAAATGCATGAGAAACCTTTTGCCATCT1050.12964403452235435No Hit
TTCGCGGGGAATGGTAAATGGATCAATTCAACAAGAAGAGATAACTATCCTA1040.12840932943166525No Hit
TGCATGGGGAATGTGGCAGAGGTGGTGGGAAGAAGAAAGTTGAGCTTTTTCC1040.12840932943166525No Hit
GATAGCGGGAGGTGCTTCCTCTATATCAAGTGAGTTTACTGATTTTAAAATC1040.12840932943166525No Hit
CATGTTGGGCTTACAGCCTAGTAGCAGTCATTAATTCAGCACAAGACGCTCC1030.12717462434097615No Hit
TTAAGGGGGACTTACCATGTGACTTTTTATTACCAAATGCATCAGTAGTGGA1030.12717462434097615No Hit
TTCGTAGGGATCAAGGAATAGTTCAGAAAACGACTTCAGACCACAGAGACAG1030.12717462434097615No Hit
AAGACCGGGCATTGGGAACACTGGAGAGTGGAATTCAGTGTAATTCAAGGCT1030.12717462434097615No Hit
CCAACCGGCCGATCTGTGGATGGGAGATGGTCCAATGATGTCTTTCTTCTTC1020.12593991925028705No Hit
CTACTGGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1020.12593991925028705No Hit
TAGCATGGGATGTGCATAAGTATTTAGCCAATACACATTTTGCTGACTTCTT1020.12593991925028705No Hit
GGCTTTGGGCACTCTATTTTATTTTTGTTGCCTGATGATATAATATAAAAAG1010.12470521415959797No Hit
GTCGACGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1010.12470521415959797No Hit
ATCTCAGGGATAGGATGGGGATGCAACTTTCAAGAGTTGCCTTGATTGGTAC1000.12347050906890888No Hit
AGCATCGGGAGCCGAGTGACACCATTGAGAATGTCAAGGCAAAGATCCAAGA1000.12347050906890888No Hit
ACTGCTGTGCAACATTAAGCCCCGCCGCTACCTGACTGTGGTCTTCGAGCAC1000.12347050906890888No Hit
ATGTCTGGAAAATACCATTTCTGCCTTTGAGTATTTCAGACCATTTTCTGTG1000.12347050906890888No Hit
TCCTAGGGGGAAATAATTCCAGCAAGGATCAGCAGGTGAAAGGTTTTTGGGG1000.12347050906890888No Hit
TCCGACAGGGTCTGTAGGCGGGAAGGAAAAATAGAGTATTATGGTTCAATGG990.12223580397821979No Hit
TTACCTGGGACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAAT980.12100109888753072No Hit
CGTCAAGGGATCTGTAATACATTCTAAAAGGATAATTGTGTCTGGGGCTCAA980.12100109888753072No Hit
ATGATGGGGGTTTTCATGTGGAGTATTCAGTTTTGTAATAAGAGGGTTGTTC960.11853168870615255No Hit
GAAGTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA960.11853168870615255No Hit
CAAATAGGGAAAAATGTGGAAGCAACTTTGTATCTCAGGTCTTAATGAACAC960.11853168870615255No Hit
TCCATAGGGCTTGAAATCTGTGGGAGGAAGGATCCTTTTTCACTCATTCAAC960.11853168870615255No Hit
TTTCAAGGGATTAGTACTGTTACTCCTCATTGCACAGGTGATAATTACCAAT960.11853168870615255No Hit
TTTGTCGGGCATGGTTGACAATTCTTTTCTTTCAGCAGCTGAAAAATTTGGG950.11729698361546345No Hit
TGGCGAGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT950.11729698361546345No Hit
TATTAAGGGTTCCTGGACCTGGTGTCCTGGTTGGGTCCAAGGTGATTTTACA950.11729698361546345No Hit
CACAACGGGAAGCTCCGCACACTGAGATGTGCATATTTAATGCAACCTCAAA940.11606227852477435No Hit
TGTAGTGGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAA940.11606227852477435No Hit
GCCAATGGGGAAACTTGAAAGGTACTGTCTGTTGTTGAGTGGGGAAAGACAT930.11482757343408527No Hit
TCGCGCGGGGGGGAGCAGGTGGGCCCAGCCTGAGCGGGGCCTCTGCACAGCC930.11482757343408527No Hit
ATTCCCAGGAAGAAATCTTAAATCTTATGACTTATCTTTAGAATGAAAAGGA920.11359286834339619No Hit
GAAGTGGGGTAAGATAAAGGATACTAAAAACAGCTAAGAACTCCAGGACTCA920.11359286834339619No Hit
GCACGGGGTCTATGCTGAGGGGTGTGAGGCTCTAGTTGTGAAGAAGCTACAA920.11359286834339619No Hit
GTGTTAGGGCCGATCTACTAGAGGGAGCTCTCTTCTTACTCATTACTGCAAA920.11359286834339619No Hit
TTCGCAGGGAATAGTCCTCTAGTTCCAGGAAACACAGTCTTTTTTTAAAAAA910.11235816325270709No Hit
TACGCCGGGATTTTAAGTGCATTGAGGATAGATGCAGGCGGGTGAGCTGCCC910.11235816325270709No Hit
GCACCGGGGAGCCCTGCTTTAATGGACTTTTTCAGAAGTGTTTTTGCTTGTG910.11235816325270709No Hit
CCAGTGGGGTACAGAGAGATCAGCTAAAAGATTTTTTACCGTTTATGGTTAA910.11235816325270709No Hit
GAACTAGGGGGAAAAGATGGAAATGTAATGTTTAACTCAAATGAAAGGAAAT900.11112345816201799No Hit
ATGGCCGGGGATGTGGGCGGCCTTGGATGAGCAGATGCTGCAGGAGGGCATC900.11112345816201799No Hit
GACCTCGGGACAAGTGACTCTTCCTTAGACAGCTAGAAAGATGCTGACTTTG900.11112345816201799No Hit
TTCAACGGGATATCATATTCAGCATTTTGTTTTCTAAGACTTATCCTGAGGA890.1098887530713289No Hit
TCTTATGGGGGACTTGGTTTCAGAAGATAGAAAGAACTTTCAAAACTCAATA890.1098887530713289No Hit
CGTGACGGGCGTTCCCGAATGTGCTTTCCTCCCTCTCCCCTGCCCACCTCAA890.1098887530713289No Hit
ATGCTCGGGCGTGCACAGCGGGGAGAGACCCTTCCAGTGCCCAGAGTGCAAC890.1098887530713289No Hit
ACAAGGGGGCACTTCAAATATAATAATGTATACAGATTAAAAGGGAAAAGTT880.10865404798063982No Hit
GGCTTGGGGACTAACTAAATATATAAGATAATTTCAGTAAGAGAGAAGTGCT880.10865404798063982No Hit
TTCCATGGGATTTGTATGATATTTTAAAATCTCCTAAATGCTGGGCAATGGC880.10865404798063982No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGCAGACTA880.10865404798063982No Hit
AGCAAAGGGAATCTCCCATATTGTAACTTACTACTCCGGAAAAAAAGAACCA870.10741934288995073No Hit
GACAAAGGGATTACTATAGTAGCATATATCCAAAGATGAAATAAAATATTAC870.10741934288995073No Hit
GCCATGGGGATGTGCAAATGAATGCCACTTACCTCCATTCCACGGTATATAA860.10618463779926166No Hit
AAAGTAGGGATTATTGATGACTAGACACCTGCTAGGATGTAAAGGCATTTAA860.10618463779926166No Hit
AGAAATGGGGATCTCAAAAAGGGCCACGGGAAGCCCTGTGCTTGCCTGGGTC860.10618463779926166No Hit
GTCTTTGGGTCGGGCCTGCCTCCTGCAAAAGAGGGGAAAGAGAAGAAGGAGA860.10618463779926166No Hit
AGTAATGGGATACATTTCAAAGAGACTGAAAAATAATTTACAAGTTTTCTAA850.10494993270857257No Hit
TACGCGGGGACTGGTGTTTATTACAGAGTTTGACATACATGGGTTCATTTGC850.10494993270857257No Hit
ACAGTTGGGTGGTCCCCTTCTAGAAGTACACATGGGCTTATACAGTAGTTCT850.10494993270857257No Hit
TGGGTAGGGCAAGAAGTTCAACTATATTCTTTTAGATATTATGTATTGTTTT850.10494993270857257No Hit
ACGTTTGGGTGCATTCCTTGGTCTTTGCCTAAATAACAGTTTTATATGCACA850.10494993270857257No Hit
AAGACATGGGCCTGAGCTGCAAGGTTCTGCCTCACCTGTCTCTCCACTCTGA850.10494993270857257No Hit
GCTAAAGGGTATGATGACTCTAAGAAATAATAATGTATTGAATACTGGTAAT840.10371522761788347No Hit
GGACTTGGGCTGCACCTGAGGGGCTAGGGACAAAATGATGAATAAGACAGGC840.10371522761788347No Hit
CGAAAAGGGTCAGAGCGTATTTGGATATGAGTATCCTTTGCTTATCTTTGTA840.10371522761788347No Hit
AAAAAGGGGGATCTGGTAGAGGGACTAGTATCCTTAATCATTTTTATTGCCA840.10371522761788347No Hit
ACTGTGGGGAACATCTGCTGTCTCAAGCACTTAAAAAAGGCTACAATTCAGG840.10371522761788347No Hit
CTACCAGGGAAGAGCCTTTACCAGAAAAGGGGATCAAGTCGGCACTTAAGCA840.10371522761788347No Hit
AGCTCCGGGAGATAAATGAGCAATTTCACAGATTCTTGCGGGAAAACTCAAA840.10371522761788347No Hit
ATAGTTGGGTGGGGGCCTATAGCCCCTTTCTTTTGGCTAATTTCTCCCTTTT840.10371522761788347No Hit
CGTCTAGGGCTGGAGAAGAGCTACGAGCTGCCTGACGGCCAGGTCATCACCA830.10248052252719439No Hit
CCGTCAGGGGCCATCCACACTCCCAAGAGTTGACCTCAAAAAAATCACTTGC830.10248052252719439No Hit
TAGACCGGGCTCAGCCCCTATATTTTAATTCTTGATCATGTAGAAATTGTTT830.10248052252719439No Hit
ATGAAAGGGACTGCAGCCTCCACCTCCATGGTTCAAGTGATTCTCAGCCTTC820.10124581743650529No Hit
TGCGGTGGGAGAAATATGCAGGTCACTTGGATATTAGATAATGAGGGAAAAT820.10124581743650529No Hit
ATCTTAGGGGATCTTGCGAGGGGATGGCCATTTTCATGATATTGATTCTTCC820.10124581743650529No Hit
GCGTGGGGGATTGTTGATTACCATGGTTGTGATTTCTTCCCTGAACGCTGGT820.10124581743650529No Hit
ACGAATGGGGTTTTCATTTCTCTTTGGTATATACCTAGGAGTAGAATGTCTG810.10001111234581621No Hit
CTCAGTGGGAGTAATGATAGTTGTACAACAATGTGAATGTACTTAATGCCAC810.10001111234581621No Hit
CACAACGGGGGGAGGTTTGTTTGATTGTCAAAATGTACAGCCACAGCCTTTT810.10001111234581621No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)