FastQCFastQC Report
Sat 31 Dec 2022
SRR5846040.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846040.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121831
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGCCGTCT10160.8339421001223005No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGC9270.7608900854462329No Hit
CTGAGCGCGGGATTGATAGCATTTATTGTGATAAGTACATTGTTTCCACTTC1410.11573409066657911No Hit
CGACAGGGACGTGGAGCACCTGACGGAGAAAATGAAGACCACCATCCAGAGA1390.11409247235925175No Hit
AAAGCGGGGCCAGTAGCACATCGAAAAGCTTATCCACCACAATCAAGTCGGC1390.11409247235925175No Hit
AGAATGTGGCCGGATCTAATGTCATACTCACATCTTGCCTATTTAGTCTGAC1380.11327166320558807No Hit
CCAAAGGAGCAAAGAGAAGAAGGAACATTATAGGGTCTGGGACTTAGAGGAA1380.11327166320558807No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGC1370.1124508540519244No Hit
TTGCTTGGGCAATTGCAATGATTTAAAGAAATGTATTTACTATAGGATAGCA1370.1124508540519244No Hit
AGAACAGGGGTTTCTAGAAGGATTTCGTCTTCCAGGGGAAGCTCAGAAAATC1360.11163004489826071No Hit
ATATCGGTGGGCAAGCAGTGTGATAAGTTCTGTCCATTCTACATACTTGATA1350.11080923574459701No Hit
TGCTATGGGCTAGTTCTTATAGAAATAACAAAGCAAATAAAAGTTCTTAACA1340.10998842659093333No Hit
ACACGACAGGGATGTCGCAAAAGATCTACACTGAGACAAAGGATTCCTCAGC1330.10916761743726966No Hit
TACACAGGGAAGGGTGCACGGACGGCCCCACTGCAGTGTGTCTCCATGGCCG1330.10916761743726966No Hit
TTTTCAGGGAAAGAGACGAATGAGAGTCCTTGGAGATCAGACCTGTTGATAG1320.10834680828360599No Hit
ACACGTGGGGGATTCTAGTAAATGAATGCAAATTAGACCTTTTAAATCAAAA1320.10834680828360599No Hit
GATCCAGGGGTTATTATTAATGAAAAGTTATTATGGCTGGATTACAGGTGAT1320.10834680828360599No Hit
TTGATAGGGCGATCTATGGAGGGACACATTTCCATTTTTCTCATAGTATATA1320.10834680828360599No Hit
CTTGGCTTGGGGTTTGATGAGCTTGTCACATTCTAATCCCTCTCCCCATCCT1310.1075259991299423No Hit
TCATGCGGGCAGCAAAAGACATATTTGTCCTCAGGATGCTTATAAGGCTAAT1310.1075259991299423No Hit
GTTCCTGGGGAAGGTACTAAGCCCCAGGACCAATGGTAGACAAAATAATTCA1310.1075259991299423No Hit
ACCGTAGGGAAGCTCCAGCTTCTCAGAAAGAATTAAAGAACTTGCTGTTCAA1300.10670518997627862No Hit
GACTTGGGGTGTAGTCAAATTTGTCATATTCCCCATATGTGATTTTTAAAAT1300.10670518997627862No Hit
TACTTTGGGGGGATGGAAGGAGGGTAGGGAAGTTATGAATTACTCCTTCCAG1300.10670518997627862No Hit
GCATGAGGGGTTCCTCATGGCAGGTACTGGGAGGGGAGATGCTCTGGGAAGG1290.10588438082261492No Hit
TCAACTGGGAGATCAGACTCATGTGCAGAATCGTGCCTGGAGAGAGAGGTTT1290.10588438082261492No Hit
CGCTATGGGATGCTTCATGTTCTTCCACCCATTTACCTAATAGTATGAAACA1290.10588438082261492No Hit
CCTTATGGGATGTGAATTTTACCTCAATTTAAAACAAAATGCCGATTGAACT1290.10588438082261492No Hit
TCTAAATGGGAGACGCCATGGCTGCATTGAATGATATACGGTATCAGAGACT1290.10588438082261492No Hit
GTTATTGGGAGCCAACCCATTTTTCTCTTCCTGTGTTGTTCACAATAACAAA1280.10506357166895125No Hit
ATTCTCGGGCTGAAATTTGGCTTTTTTTCCATAGCCACGTGCCTTCTGTTGT1280.10506357166895125No Hit
TGCCAGTTCCCTCACCCAGATCAGTAACATTTTCAGGTGGCATATTATCTGG1270.10424276251528757No Hit
CACAAAGGGATAGAGTGTCAGCTCCTTTTTAATAAGTGAGAAAAATGAGGTT1270.10424276251528757No Hit
GGCGGGGGGCCACATAAATGAACATAAAACAATCCTCAGCAAATACAAAAGA1260.1034219533616239No Hit
TTGCGCGGGTGAACTCCCATTCACAATTGCTTCAAAGAGAATAAAATACCTA1250.10260114420796021No Hit
CCAGTAGGGTCCCTGACCTCAGAAACCCTGAGAGCCCTCAGAAACCCTGAGA1250.10260114420796021No Hit
AATTCGGGGGGTGGTCCATCCCATAAGATTATAATGGAGCTGAAAAGTTCGT1250.10260114420796021No Hit
GGTAGCGGGATGGCAATGCTTAGCACAGTATCTCGGTCACACCAAATTTCTG1250.10260114420796021No Hit
GTTCTGGGGTGAGGTGTTAGAGAAAGAGAAAAGCCATAGGTAAAACCAAGGC1240.10178033505429651No Hit
TGTCGGGGTGGGCGGTTCGGGAGATACCAAAGGAGAAAAAGTACCACTGTTG1230.10095952590063284No Hit
TGAAATGGGTAGAGAAAGAAAAGCAATAATTTTACAGGCATTTTGAGGTGTC1230.10095952590063284No Hit
GTAACGGCGGGGCGGGGGCGGGGCCTGGCGCCGGCGGTGGCGTCACAAAAGG1230.10095952590063284No Hit
GCATAGGGGAGCAGAGTGTCTGCATCTCTTGGAGAGTTACACATTTCTTCAT1230.10095952590063284No Hit
ACTACTGGGCTTTGTACAAGCTTTACAGTGCAGCAGCAACCCACAGACATCT1230.10095952590063284No Hit
ATTTAAGGGGTATTCAGATTGAGAAATATGATTTTAATGCTTTAATCTCATG1220.10013871674696916No Hit
GAATCTGGGTATCAACTTATAAATGAAGAGCACATCAAGGCAAGCAGATCCA1220.10013871674696916No Hit
CATGGAGGGTATAGAAGGCGGTCACCTTTCTCCTTACTATAGTCGTGGAGGA1220.10013871674696916No Hit
TATACAGGGGCAGATTGAAATTTCTGAAAACTGAAATAATAAAACTAATTTC1220.10013871674696916No Hit

[OK]Adapter Content

Adapter graph