FastQCFastQC Report
Sat 31 Dec 2022
SRR5846031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5846031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91816
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGC6610.7199180970636926No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGCCGTCT5970.650213470419099No Hit
TGAAAAGGGGGTCAAGCTCTTGCTCATGTGCCCTCCGAGCATAGGCTACAGA1180.12851790537596933No Hit
CTCCTTGGGCAACAAATTTAAATATTCATTTACTATCTGCTAGATGACAAGT1130.12307223141936045No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGC1100.11980482704539513No Hit
TCGTCAGGGGAGTCACAAAAGAGGAAATACAAATGTCCCATAACATATGAAA1090.11871569225407337No Hit
CATATGGGGTTCAATGACGCAGTTAAGTCATCACCCAAGGATTTATGAATTT1060.11544828788010804No Hit
TCATATGGGAGATTAGTCCATTTGTAAATTGACATTCAATACAATATGTGGT1030.11218088350614273No Hit
CTGCGCGGTACAGAGAAGAGGTGCAAAAATCAACTTGATGGTAGTGGGAAGA1020.11109174871482094No Hit
CGAGCCGGGGTCTGGTCTTATGCCAAAGCATCCAAAAGAACCACGAGAGCCT1000.1089134791321774No Hit
TTATGAGGGTTTACAAAAGTTGTGCCAGACATTACAGAGTGAAAACGTCTCT990.10782434434085562No Hit
ACCTTGGGACAAGCCAGGTCGTCTTCAGTGGCACAAACCAACCCGTTGAGCC980.10673520954953386No Hit
GGCAAAGGTCTCCGCCCTGTCCACCAAGTTCCCCTTGTCCCCCATGTCCCTA980.10673520954953386No Hit
CAGTAGGGAATGTAGAAACAGTGGAATATAAGAACATCTGTTTCACAGTCTG970.10564607475821207No Hit
TCCAATGGGAATTGGCATAGACATAAAGGATAAAAGGAAAATAACCGTCTGC950.10346780517556853No Hit
AGTAGTGGGCAAATAGTTGAAACAGAAGTTTTGATTGGCAAATCAGGATGTG950.10346780517556853No Hit
CTGTATGGGTAAGGAATAAATTAGAAACAAATTAATAAGAACTAGAGCTTCC950.10346780517556853No Hit
TACAATGGGCTGGCTTCGGGATCATTTCAATTGGAAGAGATTTGGAAAGGAG940.10237867038424675No Hit
CAAAGGGGAATCTATGATGCTGATGGTGATGGAGATTTTGATGTGGATGATG940.10237867038424675No Hit
GTTCTTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT930.10128953559292499No Hit
ATTGCCGGGCTGGTCATTTTGGGGCGCTTAGGGGTGGGTGCCGGGGGGCGCG930.10128953559292499No Hit
TTATTAGGGATGGCAGCTGGGGTTCTCCTGCTGTGAGGCGTCCCGGTTCCCC930.10128953559292499No Hit
CAAAAAGAAAAAAAGAAATGAAATAATATCTCTGAATTTTAGTCCATGTTCT920.1002004008016032No Hit
CCGCATGGGGATTCCTTTATTAGCCCACATTCTGATGTTCCCTGGTGAGACT920.1002004008016032No Hit
TAATATGGGGCAAATTGTTATGGATACATTTCAGAATCTAAGAAATCAGGCA920.1002004008016032No Hit
TCTTGCGGGAGTGGCGTGATCACAGCTCACTGCAACCTCTGCGTCTCAGGTT920.1002004008016032No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)