FastQCFastQC Report
Sat 31 Dec 2022
SRR5845993.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845993.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86510
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGC7260.8392093399606982No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGCCGTCT7160.827649982660964No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGC1360.15720725927638424No Hit
CGCATTGGGGTACTGGCCTGCACCAGAAGATGTCTGCATTACTCATTGCTAA1340.1548953878164374No Hit
GCCTGGGTGACTGAGCAAGACCCCACCTCAAAAAATAATAATCCAGCCATGG1300.15027164489654377No Hit
AACGTTGGGAGAAGTGACAGCGCTGTATTCATTTGAAGGACAGCAGCCTGGG1280.14795977343659694No Hit
CACATCGGGTAAAGGTTTGAGTTTCATGAGAAAAGAAGACTTTGCAGAGTGG1260.14564790197665012No Hit
AAGACAGGGATCAAAAAAGATCTTTAAAGTATTCAGAAGCAACAGAAAGAAT1250.14449196624667668No Hit
TTATGAGGGTACTGAATAACTGAAATGTCAGCAATTTAAAATAAATTCAATT1220.14102415905675644No Hit
GTATGAGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT1220.14102415905675644No Hit
CAACTGGGTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT1210.13986822332678303No Hit
TACAAATGATTACTACAGGAATAGTGGCCACTTAATGTCAGTTACTCCGGTG1210.13986822332678303No Hit
GAACTAGGGGCAAAGAAGCTGCAAAGAGCGACCCTTCCATTGTATTACTTCT1200.13871228759680962No Hit
CTGACTGGGCAAGTTTTTCAATCTAACTAGAAGAGGTTGCGGTGCTGTAATG1170.13524448040688936No Hit
AGAAAAGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG1170.13524448040688936No Hit
ATGAGTGGGCAGAAGGTGGCGCGGCAAAGGGCCTCGTGCAGTGTGTTCAGAT1140.13177667321696915No Hit
AAAAACAGGGAGGAAACATGAAGCATAATTTGTTTAAAATTCTCTTTGGTCA1130.13062073748699574No Hit
CTTTAAGGGTCTTGAGCAGAATTACCAATGCATTTGTCTTGAATGCTTAATA1120.1294648017570223No Hit
TACGCTGGGTGCAGAAGGAAGCTATTGCTGCTCACAAGGAAAACAAGCTACA1120.1294648017570223No Hit
CGTTGCGGGTGTTGAACCAGAGCTAGACATCTCAGACCAAAAAAGAATAGAT1120.1294648017570223No Hit
GCGTATGGGAATTAAGACAGAGTTCTGTTAATCTAATCAAGTTTGCTGTTAG1110.1283088660270489No Hit
GCTAATGGGACAAGTGTCCAGTCTGGATGTCAAATTTTCATTAAGGGCATAG1110.1283088660270489No Hit
GGATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1100.12715293029707547No Hit
TACAGAGGGAGTGTTCTCTAGGTTTGTGAGCAGTTGTGGTAAGAGAATGGAA1100.12715293029707547No Hit
GAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.12715293029707547No Hit
GTGGCGGGGAGGGAAGGTTTCCTGCTGATTCTGGTTATACATTCCTGTAGAA1100.12715293029707547No Hit
ACGTATGGGCATGGTCAATTTTTAAACACGCATCAAGATCTGGAAGGCAAAA1090.1259969945671021No Hit
TGCAGGGGAAAGATGAAGAGGCAGAATTGAGAGGCTAACATATTTACTCTTG1080.12484105883712865No Hit
CGTACAGGGCTGAAACTCAAGCCTCAGAAACCTCTAGACCCAGCTCCCCTGA1080.12484105883712865No Hit
CTTGAGGGCTGTATGTTAATTTCAGGAGCAAGGCGAGGATGTCCTCCAATTC1070.12368512310715525No Hit
TTTGTGGGGATACTCTCAGTTATATCAACAGGAGTCTTGTGTTTTGTTACCT1070.12368512310715525No Hit
CGTGGAGGGAGGTCTGGAAGGTTTGAAACATTTTCAAATAATTACTTGGTCA1070.12368512310715525No Hit
CTTTTGGGGAGGAGGAGGCCATGACTCAACAGTTCTCCCAACCTGTTGGTGA1070.12368512310715525No Hit
CAAAATGGGGAACTCCTGACCTCAAGTGATCCACCTGCCTCGGCCTCCCAAA1060.12252918737718183No Hit
CGTTGCGGGGGTGTTACTGAAGGGATATGGGAAATGTCGTGGACGGCCAGGG1060.12252918737718183No Hit
CAGGTGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAG1050.12137325164720841No Hit
TGTAATGGGGCACACGCTGGTCACGCGGTCAGCTATTGACACTTCCTGGTGG1050.12137325164720841No Hit
TGGCCAGGGGAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCT1050.12137325164720841No Hit
GTTCTGGGGGCAGTAACAACAAAATTTGTTCGGACATCAACAAATAAAGTAA1050.12137325164720841No Hit
AACTCCGGGATTAAAAACATTTTCAGCTTCATCAGATGCTGACTAACTGACC1040.120217315917235No Hit
CAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA1040.120217315917235No Hit
TTGCGCGGGCAAAGAAGCTTTTTCACTATTTGACAAAGATGGTGATGGAACT1040.120217315917235No Hit
GATAATGGGAGAGGAAGTCAAGGAAGAGTATGAATGTGAGTGTGGGGCAGGG1040.120217315917235No Hit
CGTATGGGGGACGGATGGTCTGTGACTGTGAGAATCCCACAGTTGATCTTTT1030.11906138018726159No Hit
GACTCCGGGTATTTAATTTTTGCATATTGTGGATTTCCCAGTTTTCCTTCTG1030.11906138018726159No Hit
CTGCCGGGGAACAGTTGGCTACCTTTAATTGTCCAAAGCTTGGTGATTGGCA1030.11906138018726159No Hit
AACCTAGGGGAACAAGATGGTCTAGATTACAATAAGGACCCCATGGGACAGA1020.11790544445728816No Hit
ACGTTCGGGAAGTGACAAACAGGCAGGGAGCGTGGACAGTGTGGATACCCTG1020.11790544445728816No Hit
CAACTAGGGGCAATAAATTTTAATCAACTATTTTTCAACTTTATTTCTGCAG1020.11790544445728816No Hit
CTTAGGGATCAAAAGAGTGTGACTTCTCATTTGTGAGTAGTTCACAAATTTC1020.11790544445728816No Hit
AAAATAGGGCGAGGAATGGGAGGTAAAAAGTGGGGGCATTGCATTGGAATAG1010.11674950872731477No Hit
AGCAGCACTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTA1010.11674950872731477No Hit
CAAAATGGGGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATAT1010.11674950872731477No Hit
TTTGCTGGGGTATGCATTGCTGCGTGGAGGCTGTTTATTTCAACCTTTTTAA1010.11674950872731477No Hit
AAACTGGGGCAATTTGAGATCTTTCTAGCTTTCTGATGCGGGCATTGAGTGC1000.11559357299734134No Hit
ATAACAGGGTCTATACAAGGGAGCATTTTTATGAACTTTGTGTTACAGTTTT1000.11559357299734134No Hit
AGTCAGGGGTCAAAAGGAAAAGAACTACCAATGAAATAAAAAATCTTCAGTA990.11443763726736794No Hit
CGACAGGGCACTAATACAAGTTTAAATTTTTCAGAAAAATCAATCCCCAGTG990.11443763726736794No Hit
CTAGGTGGGAAGTGAATGAATGAGGCAGCATTGCTAATTTTTGTATAGTGAG990.11443763726736794No Hit
CGCTGAGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG990.11443763726736794No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCAACTCGTATGC990.11443763726736794No Hit
CGTACGGGGGATGGTAAATTCTGCTTGGTTGTTTATAAGCTCAAACCTTGGA980.11328170153739452No Hit
GCATCGGGCAGGCACCATCATCTTTTGGCTTATGCTCAATGTTTGCCTACTG980.11328170153739452No Hit
TCCGTGGGGGAGATACAGTTGCCCCTATATGATCCTGTCTTAGTCCATTCAG980.11328170153739452No Hit
GTGGGGGGAAGACAGACCTCTTGAAAGTTAATGTTTCAGTTACAAATCACTA980.11328170153739452No Hit
GCATATGGGTCACTGCTAACACTCTCACTTCTTCATCACGTCTCCTTCTGAG980.11328170153739452No Hit
ACTAGTGGGGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCCAAGGAGAAAG980.11328170153739452No Hit
TGGCTAGGGATAGCCCTCGTAGTAACAGCCATTCTCATCAAAACCCCCTGAA980.11328170153739452No Hit
TTGTTCGGGCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCATGATCTG970.1121257658074211No Hit
CATATAGGGATGGAGGGGCTTTAGCCATCAGCTTTGTACATCATCATTTTTC970.1121257658074211No Hit
CGCACAGGGATTACCTTTCCAGTAAAAAGTATAGATAATTTAATTAACTTAG970.1121257658074211No Hit
GCCTCTAGGGGTGAAAATTCTTAGCAATAAACTAAAATCAGGAATTCTGATT970.1121257658074211No Hit
ATAGTAGGGGTGCCCTCTTTGTCTACGAGAAGGGGGCTCGGGTGCTTCTGGT970.1121257658074211No Hit
AGGCTCGGGGCAATTCAATTTAACCCAAAGAGGGAGTCCTGGGAACCCCAAT970.1121257658074211No Hit
GTATGAGGGATTCCACCCATCTTTACAGCAGAAGAATACATTACTTCCATTA960.1109698300774477No Hit
GTATCAGGGTCTGCTTATGACAATAATTGGCCCATTGCATGCAGAGAGAATG960.1109698300774477No Hit
GACGCTGGGCTTCCGATCTAATGCTGGGCTACCTCTGTGTTTCACATGGCCT960.1109698300774477No Hit
AATTCAGGGAAGAGAAAAAAACAGAAACAAAGTAAATGTTCACCAACAGGAG960.1109698300774477No Hit
GCGAAAGGGGCTCCTGTCTTCTGGCTTCAGTCTGGAAGATCCCCAGACACAT960.1109698300774477No Hit
AGCGCTGGGTACGTGAAAAATGATACTCTGCAATTTTAACTTATATTTCTTT950.10981389434747428No Hit
GTCATTGGGAAGCCGTGATCAGGTAACACGCAGTTTCTTAGTAGCTGCCTCT950.10981389434747428No Hit
GCGTACTGGGACCGAAGGCATTCGTGGCTTCTATAGAGGATTAACTGCCTCG950.10981389434747428No Hit
ACGCTAGGGACTGTCATCTGCTTTATAATAATGCTCAAGATGCCTGATAAAA950.10981389434747428No Hit
TGTCTGGGGACCTGAAGAGACTCTGAAGACCTACTGGGAGGATAACAAAAAC950.10981389434747428No Hit
TAGCATGGGAGACTGATTGTATATAGTAAGAAAAGATCAAAAGACTTTAAAA950.10981389434747428No Hit
AGCTTTTCCCAAAAGGCCAAGGAAGGGATGGTGTAGGTTCAAAAGGGAAACC950.10981389434747428No Hit
TTATAGGGGGAAATGTAATATTGACACACCACTATTATCTAATTACAATATA950.10981389434747428No Hit
TGCAGGGGGGAGCGTGAATGACATGGCCCTGGATGCCTTCGACTTGGACCGG950.10981389434747428No Hit
TCTGATGGGCACTAGGCCAAAAGAGCCAAAGGAAGTGAGTGAAGTTATCCTA940.10865795861750088No Hit
AAATGGGGGGTGATACTGAATATCCAGCATTCGTCTCTGAGCGCACCATCAA940.10865795861750088No Hit
TAATCTGGGGACTGTAAGTGGCTTCCGGGTCTGCGCGCACAGTGGCGGATGA940.10865795861750088No Hit
AATGCAGGGGGTACTTGCATTTGACTTACGATGTATCTGTGAAAATGGGATG930.10750202288752746No Hit
GACACAGGGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGTGATTCTCCTG930.10750202288752746No Hit
TCAGCAGGGCTTTTTAAAATGGAGGCCAAAAATACATTTTCCAGATCAACAA930.10750202288752746No Hit
ACAAAAGGGATCTGACCTATGGAGGTTGCCCTCTTTACTCCTCTTCCCTAAA930.10750202288752746No Hit
GGATCTGGGTGCTTTATGCTCATAACTGATGTGGCTGGAGAATTGGTATTGA920.10634608715755405No Hit
TTCTACGGGAGGAACCATTCTTATACTACCCTTTACTGGTAATTTCTGTAGA920.10634608715755405No Hit
TATGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.10634608715755405No Hit
CGTATAGGGGGTCCTAGGATTCCTGAGCCTGTGAAGAATCAAGCTCCTATGG920.10634608715755405No Hit
CTCTGATGGGAGGTGGTACTTAATAATGTAGACTAGATAGCAGTAGAAACAG920.10634608715755405No Hit
CATACCGGGTTGAATGAATTAGTTCTGTTCTGTGTCAAATATGGCCATAACA910.10519015142758062No Hit
AGCCTTGGGCGAGTTGCGGGAACAGATGTCAGGTTATAAGCGGATGCGGCGC910.10519015142758062No Hit
GGTGCTGGGATCCAAGCATAGAACCTGATCTGACTCACCTTTACCACAGTTA910.10519015142758062No Hit
GTATACGGGTAGCATAAATATCAGGTTCTTAGAGGTCTTTAAGAGAATACTT910.10519015142758062No Hit
GTGCTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA910.10519015142758062No Hit
CATTTCGGGAGAACCTGCATTTAAGAACAGAACAGCAAGTATGAACCACATG910.10519015142758062No Hit
CTACATGGGGCAGTCCCCCTGTGGTGTCTTCAAAGTGCTTTTGCATATGAAT910.10519015142758062No Hit
CAAAGTGGGAGAGCTAGTGAATTAGCCTATTTGTAAATACCTTTGTTATAAT910.10519015142758062No Hit
GCACAAGGGAGAGGAAGAATGGAGGAAGAGAAAAATACTTTAGACCTCAAAT900.10403421569760722No Hit
GCTACAGGGATGGCACCAATATCCAAAGAGTTCAAAGTGTTTTTAATTGTTG900.10403421569760722No Hit
TCAAGTGGGGGACGCTCTTCCGATCTGTAAGCGGGGAAATAGGTTATAGGTA900.10403421569760722No Hit
ATAGCCGGGTCTTCCGATTCTGTTAAAGGGTTAAGGAAAAGCGGGTCATTGC900.10403421569760722No Hit
TCTCGAGGGCCAAACACCATCCAAAAGCTGCATGCACTTCCTCCTCGTTTCA900.10403421569760722No Hit
ATCACAGGGGTTGTTTGAGTTTTGTATAATGATCTTATTTATTTTTAAAAAA890.1028782799676338No Hit
AGCACCGGGATTTGTAAGAATTGGAGCAATGTTGGCCTATACGCCCCTTGAT890.1028782799676338No Hit
TCATTGGGCCTCCATCCCTCTCTGAGATTTATCAGTGGAAACTTGGACCCAC890.1028782799676338No Hit
AACGCTGGGACCTTTTGGGGTCCCCAGTGTTGTTTCCTTCCTTATGTTCATG890.1028782799676338No Hit
AAAATGGGGAAAGTTAGATAATGGGTTTTATGGATTTCTTTGTTATAATATA890.1028782799676338No Hit
GGCTGAGGGTACCACAGACTGTGGCTTAAACAACAGAAATAACAAAGTAGCA890.1028782799676338No Hit
TCTCGCGGGGGCGCCGTCTCGCGCCGCACGCTCTGCGGGTTCCTCCCTTCTT890.1028782799676338No Hit
GGCAATGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA890.1028782799676338No Hit
ATGGCTGGGAGCCTTTTATAACTCTAGGAGTTGAAGTGGAATTTTATCATTG880.1017223442376604No Hit
TCATCAGGGCTTTGTAAATAGTCATAATAATTGCTACTGCTACAAAGTACTA880.1017223442376604No Hit
GCACATGGGATGCATCTACTCAAACCACCCATGAACTCACCATTCCAAATAA880.1017223442376604No Hit
TATTTTGGGATCCTCATCTGGGACAAAAGCCTGGAGGTTCAGATTTCTTAAG880.1017223442376604No Hit
GAGCATGGGCTGCCGGGCGCTCCGCGAAGATGCAGCTCAAGCCGATGGAGAT880.1017223442376604No Hit
TTTTCTGGGATCTTCTAGAATTTTTATGGTTTCAGGTCTTAGATTTAAGACC870.10056640850768697No Hit
TCAGCAGGGTCCAAGGTGATTTTCTTTTCGTATGAATACAGGTCCTAGGGAT870.10056640850768697No Hit
TCGTAAGGGTTCTTATGCTGTAACAGTTAGCATGGCTTCACACTGTTTGTGT870.10056640850768697No Hit
TTTAGAGGGTCAATGCCCACGAGCAGCAAACCCAGCAGCTGAAACTGTCCCA870.10056640850768697No Hit
TTTAGGGGGTTCTTTGTAATGTTCTTTGTTGCCATATGGTATTGTGGTCATA870.10056640850768697No Hit
AATAAAGGGTGCCTGGCCTGCGACATGCTAATTCCTGCACAGCCGCAGCTCC870.10056640850768697No Hit
CTAGTAGGGTAGAATGATTATAGTGATTTTATATTATTTGTAGAAAGACATG870.10056640850768697No Hit
GGCAAAGGGGATTGTCACTGTGGAGGCTTCTAAGCCAAATGGACAGAGGTAT870.10056640850768697No Hit
TCGGATGGGGAACTATCATAGTTGAACAACTAATGATAAAACACTAGGTAGA870.10056640850768697No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)