FastQCFastQC Report
Sat 31 Dec 2022
SRR5845992.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845992.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85646
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGC7730.9025523667188192No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCTCGTATGCCGTCT6960.812647409102585No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGGAAGC1430.16696634985872078No Hit
AAGACAGGGATCAAAAAAGATCTTTAAAGTATTCAGAAGCAACAGAAAGAAT1350.1576255750414497No Hit
TTATGAGGGTACTGAATAACTGAAATGTCAGCAATTTAAAATAAATTCAATT1310.15295518763281415No Hit
CTTTTGGGGAGGAGGAGGCCATGACTCAACAGTTCTCCCAACCTGTTGGTGA1310.15295518763281415No Hit
CTAGGTGGGAAGTGAATGAATGAGGCAGCATTGCTAATTTTTGTATAGTGAG1300.15178759078065526No Hit
TGTAATGGGGCACACGCTGGTCACGCGGTCAGCTATTGACACTTCCTGGTGG1300.15178759078065526No Hit
CGTACAGGGCTGAAACTCAAGCCTCAGAAACCTCTAGACCCAGCTCCCCTGA1290.15061999392849637No Hit
ATGGCTGGGAGCCTTTTATAACTCTAGGAGTTGAAGTGGAATTTTATCATTG1200.1401116222590664No Hit
AGTCAGGGGTCAAAAGGAAAAGAACTACCAATGAAATAAAAAATCTTCAGTA1200.1401116222590664No Hit
CTGACTGGGCAAGTTTTTCAATCTAACTAGAAGAGGTTGCGGTGCTGTAATG1200.1401116222590664No Hit
CGCATTGGGGTACTGGCCTGCACCAGAAGATGTCTGCATTACTCATTGCTAA1190.1389440254069075No Hit
CATATAGGGATGGAGGGGCTTTAGCCATCAGCTTTGTACATCATCATTTTTC1170.13660883170258975No Hit
TACGCTGGGTGCAGAAGGAAGCTATTGCTGCTCACAAGGAAAACAAGCTACA1160.13544123485043086No Hit
TCAAGTGGGGGACGCTCTTCCGATCTGTAAGCGGGGAAATAGGTTATAGGTA1150.13427363799827197No Hit
CAGGTGATCTGCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAG1150.13427363799827197No Hit
TTTGTGGGGATACTCTCAGTTATATCAACAGGAGTCTTGTGTTTTGTTACCT1150.13427363799827197No Hit
ACGTATGGGCATGGTCAATTTTTAAACACGCATCAAGATCTGGAAGGCAAAA1140.13310604114611307No Hit
TTGTCAGGGACACAAATCCATTATTACTTATAAAGGTAAATTATACTATAGA1140.13310604114611307No Hit
TTTTCTGGGATCTTCTAGAATTTTTATGGTTTCAGGTCTTAGATTTAAGACC1130.13193844429395418No Hit
AGCTTTTCCCAAAAGGCCAAGGAAGGGATGGTGTAGGTTCAAAAGGGAAACC1130.13193844429395418No Hit
GCGTATGGGAATTAAGACAGAGTTCTGTTAATCTAATCAAGTTTGCTGTTAG1120.1307708474417953No Hit
GCATCGGGCAGGCACCATCATCTTTTGGCTTATGCTCAATGTTTGCCTACTG1110.12960325058963643No Hit
AACTCCGGGATTAAAAACATTTTCAGCTTCATCAGATGCTGACTAACTGACC1110.12960325058963643No Hit
AATTCAGGGAAGAGAAAAAAACAGAAACAAAGTAAATGTTCACCAACAGGAG1110.12960325058963643No Hit
GCCTGGGTGACTGAGCAAGACCCCACCTCAAAAAATAATAATCCAGCCATGG1110.12960325058963643No Hit
AGGCTCGGGGCAATTCAATTTAACCCAAAGAGGGAGTCCTGGGAACCCCAAT1110.12960325058963643No Hit
AAAATAGGGCGAGGAATGGGAGGTAAAAAGTGGGGGCATTGCATTGGAATAG1100.12843565373747753No Hit
GATAATGGGAGAGGAAGTCAAGGAAGAGTATGAATGTGAGTGTGGGGCAGGG1100.12843565373747753No Hit
CACATCGGGTAAAGGTTTGAGTTTCATGAGAAAAGAAGACTTTGCAGAGTGG1090.12726805688531864No Hit
CGTATGGGGGACGGATGGTCTGTGACTGTGAGAATCCCACAGTTGATCTTTT1080.12610046003315975No Hit
AGAAAAGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG1080.12610046003315975No Hit
CGACAGGGCACTAATACAAGTTTAAATTTTTCAGAAAAATCAATCCCCAGTG1080.12610046003315975No Hit
ATAACAGGGTCTATACAAGGGAGCATTTTTATGAACTTTGTGTTACAGTTTT1070.12493286318100087No Hit
TACAAATGATTACTACAGGAATAGTGGCCACTTAATGTCAGTTACTCCGGTG1070.12493286318100087No Hit
CTTGAGGGCTGTATGTTAATTTCAGGAGCAAGGCGAGGATGTCCTCCAATTC1060.12376526632884198No Hit
AACCTAGGGGAACAAGATGGTCTAGATTACAATAAGGACCCCATGGGACAGA1060.12376526632884198No Hit
GTATCAGGGTCTGCTTATGACAATAATTGGCCCATTGCATGCAGAGAGAATG1060.12376526632884198No Hit
GCTAATGGGACAAGTGTCCAGTCTGGATGTCAAATTTTCATTAAGGGCATAG1060.12376526632884198No Hit
TTGTTCGGGCGTGTTAGCCAGGATGGTCTTGATCTCCTGACCTCATGATCTG1050.12259766947668307No Hit
CGCACAGGGATTACCTTTCCAGTAAAAAGTATAGATAATTTAATTAACTTAG1050.12259766947668307No Hit
TGGCCAGGGGAGTGACCCGGAAGTAGAAGTGGCCCTTGCAGGCAAGAGTGCT1050.12259766947668307No Hit
CACTTTGGGATATTTTATTCATTTTACTTGCCTCTGGTGGTGCAACTGGGTA1040.12143007262452421No Hit
TCTGCTGGGACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCTTGAAAATG1030.12026247577236532No Hit
TCTGATGGGCACTAGGCCAAAAGAGCCAAAGGAAGTGAGTGAAGTTATCCTA1020.11909487892020643No Hit
TCGAATGGGGTGGCCTTGGTCATTGTGGGAGACTTCTCTGTTGTTAATTACT1020.11909487892020643No Hit
CAAAATGGGGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATAT1020.11909487892020643No Hit
GTCATTGGGAAGCCGTGATCAGGTAACACGCAGTTTCTTAGTAGCTGCCTCT1010.11792728206804755No Hit
CAGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACA1010.11792728206804755No Hit
GTAGATGGGCGAAAGGACTGCAGGTTCACTTTGCTTTGGATTACATGCCCAG1000.11675968521588866No Hit
TGTGAGGGAATCAGTTAACGTATAAAATGTACATTGTATGAGAATAATGTAA1000.11675968521588866No Hit
AATTTGGGATCCTTTTCAGAAAAAATATAATTGCAAATAGGTAGGAGTGGCC1000.11675968521588866No Hit
GACGCTGGGCTTCCGATCTAATGCTGGGCTACCTCTGTGTTTCACATGGCCT1000.11675968521588866No Hit
ATCACAGGGGTTGTTTGAGTTTTGTATAATGATCTTATTTATTTTTAAAAAA990.11559208836372976No Hit
GCTTCGGGGTAAGCAAAAATGTAAAAAGTATATGTTGATGGGACAAGAAGAA990.11559208836372976No Hit
GTATGAGGGTCGCCAGAGGTGCTTCACCCTGAGTTTTGTTTTGTATTGTTTT990.11559208836372976No Hit
GAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC990.11559208836372976No Hit
CATACCGGGTTGAATGAATTAGTTCTGTTCTGTGTCAAATATGGCCATAACA980.11442449151157089No Hit
CGCTGAGGGCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACAGAG980.11442449151157089No Hit
AAACTGGGGCAATTTGAGATCTTTCTAGCTTTCTGATGCGGGCATTGAGTGC980.11442449151157089No Hit
TATTTTGGGATCCTCATCTGGGACAAAAGCCTGGAGGTTCAGATTTCTTAAG980.11442449151157089No Hit
CATACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.11442449151157089No Hit
CGTGGAGGGAGGTCTGGAAGGTTTGAAACATTTTCAAATAATTACTTGGTCA980.11442449151157089No Hit
CTCTGATGGGAGGTGGTACTTAATAATGTAGACTAGATAGCAGTAGAAACAG980.11442449151157089No Hit
TTGCGCGGGCAAAGAAGCTTTTTCACTATTTGACAAAGATGGTGATGGAACT980.11442449151157089No Hit
GCAAGTGGGATTTGAGAATGACATCAGATTCTAAATTGGTGAATCTGAAGGC970.113256894659412No Hit
CGAACCGGGGCCACCACCCCTGCCTCTAGAACAATGCAACCAACCTTAACCA970.113256894659412No Hit
TTTAGGGGGTTCTTTGTAATGTTCTTTGTTGCCATATGGTATTGTGGTCATA970.113256894659412No Hit
CAAAATGGGGAACTCCTGACCTCAAGTGATCCACCTGCCTCGGCCTCCCAAA960.1120892978072531No Hit
GGATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.1120892978072531No Hit
AGTAACGGGGTATCAAGACCTATGCCACATCACACTGGGGCTAGGGAAGTAG960.1120892978072531No Hit
TGTCTGGGGACCTGAAGAGACTCTGAAGACCTACTGGGAGGATAACAAAAAC960.1120892978072531No Hit
CGTATAGGGGGTCCTAGGATTCCTGAGCCTGTGAAGAATCAAGCTCCTATGG960.1120892978072531No Hit
GCATATGGGTCACTGCTAACACTCTCACTTCTTCATCACGTCTCCTTCTGAG960.1120892978072531No Hit
GCGTATGGGACTTAAATGAATTAAAGACCAAAATATAAGACCCCGAACTACA960.1120892978072531No Hit
TGGCTAGGGATAGCCCTCGTAGTAACAGCCATTCTCATCAAAACCCCCTGAA960.1120892978072531No Hit
CGTTGCGGGTGTTGAACCAGAGCTAGACATCTCAGACCAAAAAAGAATAGAT960.1120892978072531No Hit
ATGAGTGGGCAGAAGGTGGCGCGGCAAAGGGCCTCGTGCAGTGTGTTCAGAT950.11092170095509422No Hit
GTATGAGGGATTCCACCCATCTTTACAGCAGAAGAATACATTACTTCCATTA950.11092170095509422No Hit
ATAGTAGGGGTGCCCTCTTTGTCTACGAGAAGGGGGCTCGGGTGCTTCTGGT950.11092170095509422No Hit
GTACACAAGGGGCAGGACGATGCTCACATTTTTTGCACAGTGGAGCAGGTAA950.11092170095509422No Hit
TAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11092170095509422No Hit
TCTCGCGGGGGCGCCGTCTCGCGCCGCACGCTCTGCGGGTTCCTCCCTTCTT950.11092170095509422No Hit
TAAAGAGGGTGTCTGTATTGCTAGAAGACACAAGTCTCTCTACTTTCTTGGA940.10975410410293533No Hit
AACGTTGGGAGAAGTGACAGCGCTGTATTCATTTGAAGGACAGCAGCCTGGG940.10975410410293533No Hit
CAACTGGGTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAAT940.10975410410293533No Hit
CGTACGGGGGATGGTAAATTCTGCTTGGTTGTTTATAAGCTCAAACCTTGGA930.10858650725077644No Hit
GGAGTGGGGGACTTACCTGTTCAGTACTCTACAATTCCTCTGATAATATATT930.10858650725077644No Hit
CTAGTAGGGTAGAATGATTATAGTGATTTTATATTATTTGTAGAAAGACATG930.10858650725077644No Hit
GGCAAAGGGGATTGTCACTGTGGAGGCTTCTAAGCCAAATGGACAGAGGTAT930.10858650725077644No Hit
ACACAAGGAATGATTTTTAACAACTATCTTCAAAGCTGCCTTTCACTGGCAT920.10741891039861758No Hit
TCAGCAGGGCTTTTTAAAATGGAGGCCAAAAATACATTTTCCAGATCAACAA920.10741891039861758No Hit
TCCGTGGGGGAGATACAGTTGCCCCTATATGATCCTGTCTTAGTCCATTCAG920.10741891039861758No Hit
TGACCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10741891039861758No Hit
AACGCTGGGACCTTTTGGGGTCCCCAGTGTTGTTTCCTTCCTTATGTTCATG920.10741891039861758No Hit
TCAGCGGGTGACTAAAGAAGTTATATCAAAAGTCAATTATTTCCCTTAATAT920.10741891039861758No Hit
AGCGCTGGGTACGTGAAAAATGATACTCTGCAATTTTAACTTATATTTCTTT910.10625131354645867No Hit
GCTTATGGGGGAAATAACTACTGTAGACTATTGAAGAATGATCTCTTTGTGA910.10625131354645867No Hit
CTTTAAGGGTCTTGAGCAGAATTACCAATGCATTTGTCTTGAATGCTTAATA910.10625131354645867No Hit
ATAGCCGGGTCTTCCGATTCTGTTAAAGGGTTAAGGAAAAGCGGGTCATTGC910.10625131354645867No Hit
AATAAAGGGTGCCTGGCCTGCGACATGCTAATTCCTGCACAGCCGCAGCTCC910.10625131354645867No Hit
ACTAGTGGGGCCAAGCTAGTCCGGGATGCCTTTGCCCTGGCCAAGGAGAAAG910.10625131354645867No Hit
TTTGCTGGGGTATGCATTGCTGCGTGGAGGCTGTTTATTTCAACCTTTTTAA910.10625131354645867No Hit
CTAAGTGGGTATCTATATGATATTGTTTTATGATTAAGGGGGCATTTCAAAG910.10625131354645867No Hit
GGCAATGGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAA910.10625131354645867No Hit
GGATGTGGGATTTCTGAGTAAGGGGCTTTGTTGAAAGAGGGGGTTAGAGAGA900.10508371669429978No Hit
AAAATAGGGATTCCACTACCACTGGGGACTGTCACCATCCTCTGCATTGACT900.10508371669429978No Hit
TTCGTCAGGGGAAGAGAGTGAAACTTCAAGTCCTAATAAAGCTCAGGGACAA900.10508371669429978No Hit
TTACCTGGGGGTGAAACCCTGTTTCTATAAAAAATAATCTGGGCTTTGTAGC900.10508371669429978No Hit
TCTACTGGGAAGTTGATAAAAGGAAGAGTTTAATAAACTTCCTAATGGAAGA900.10508371669429978No Hit
ACAAAAGGGATCTGACCTATGGAGGTTGCCCTCTTTACTCCTCTTCCCTAAA900.10508371669429978No Hit
AGCCTTGGGCGAGTTGCGGGAACAGATGTCAGGTTATAAGCGGATGCGGCGC890.10391611984214091No Hit
AAATGGGGGGTGATACTGAATATCCAGCATTCGTCTCTGAGCGCACCATCAA890.10391611984214091No Hit
ATAGACGGGAGCCAAGGGGACCAGATAACTCAATGGGGTTTGGTGCAGAAAG890.10391611984214091No Hit
GTGGGGGGAAGACAGACCTCTTGAAAGTTAATGTTTCAGTTACAAATCACTA890.10391611984214091No Hit
TTATAGGGGGAAATGTAATATTGACACACCACTATTATCTAATTACAATATA890.10391611984214091No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGGAAGCATCCTGTCTCTTATAC890.10391611984214091No Hit
TGTACTGGGTCCCTAGGGGTACACAAGTCAATAAATGTGATACATCGCATAA880.10274852298998202No Hit
GCTAATGGGCAGTGTAGAATCTTTTTGAAATCATATCAATGAACTTTTTGTA880.10274852298998202No Hit
GTTCTGGGGGCAGTAACAACAAAATTTGTTCGGACATCAACAAATAAAGTAA880.10274852298998202No Hit
AAAAACAGGGAGGAAACATGAAGCATAATTTGTTTAAAATTCTCTTTGGTCA870.10158092613782313No Hit
GACACAGGGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGTGATTCTCCTG870.10158092613782313No Hit
ACGTTCGGGAAGTGACAAACAGGCAGGGAGCGTGGACAGTGTGGATACCCTG870.10158092613782313No Hit
CATACGTGGTCAGACCATAATTATGTTCCTGGAAAATTCTAAGTGACACTGT870.10158092613782313No Hit
TTGAGCGGGAGGGCAGGGATCCCTTTTATCTTTAACTGTTAATTTCTTTCTC860.10041332928566425No Hit
CGTTAGGGTACACAGGGCACGATAGAATGTTACTGATGATTCAGGGCTATTT860.10041332928566425No Hit
TATGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.10041332928566425No Hit
AGCCATGGGACCTCACCAACATCTGTTATTTTTTGACTTTCTCATAATAGCC860.10041332928566425No Hit
TAACTCGGGGCTATACTACTGATGTAATAAAAGAGCAGGGTTAAAAATATTG860.10041332928566425No Hit
AATTCAGGGCTTTGAAATGACAATTTATTAAGCAATGGTATAAAAAACCTGA860.10041332928566425No Hit
GCTTCTGGGGCTCTGTGTCCATGTACTTAATATTTTAAAGAGCTCTTACTTT860.10041332928566425No Hit
GCGAAAGGGGCTCCTGTCTTCTGGCTTCAGTCTGGAAGATCCCCAGACACAT860.10041332928566425No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)