FastQCFastQC Report
Sat 31 Dec 2022
SRR5845968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120301
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT4840.402324170206399RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4540.3773867216398866No Hit
AATTATGGGATGCTGCCTGCAGCCATGAAGGGCCTCGGCCTGGCGCTGCTGG1740.14463720168577154No Hit
TCACGTGGGAGGGGGGCCGTCTGCAGCGCGTGGAGGTGACCTGGCGAGGCTC1670.13881846368691864No Hit
GCTAAGGGGGAGTCATGATCACCGAGACCGCGGCGGAGCCTACGGTCCCTGC1650.13715596711581782No Hit
GAATTCGGGGGACTTTGCGGAGCCATGGAGGGCGGCTTCGGCTCCGATTTCG1510.12551849111811206No Hit
GACTACGGGACTGGAATCTGCAGCCCCCACATGCATCTGTCTAACGCATGCC1500.12468724283256165No Hit
CTGACAGGGGGCCGGTCGCCCAGACATGGCGGACGGCAGCGGCTGGCAGCCG1480.12302474626146084No Hit
ACTCTAGGGGAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAAC1480.12302474626146084No Hit
AAGCTAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1470.12219349797591042No Hit
AGTCAAGGGACACGGACCAGTGTGCAGAAGGCTCCGGGAACCGCAGATTATG1450.1205310014048096No Hit
CATCCAGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1430.11886850483370878No Hit
ACCAAAGGGAGTTTGATCCATTTCCATCCATCTGCTCATGAGAGAAGAAATT1400.11637475997705755No Hit
TGGGAGGGATTCAGCGGGTGCCAAAGCCTTTGTCTGTGATCAGTGCGGTGCA1380.11471226340595672No Hit
AAGCTGGGGATTCTGGGTCCTAGATTACTGTCATTGTCTAAAAGTAACTTTT1370.11388101512040633No Hit
AAACCTGGGATCTAAAACTGGCATGGAATAAACCAGTAACTAATATTTCAGC1360.1130497668348559No Hit
GGCTTGGGAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCAGAATCC1350.1122185185493055No Hit
GCTGCAGGGATACAAAAAACCCCCATGACCCATGTTTACCTATGTAACAAAC1340.1113872702637551No Hit
CGTGATGGGTATGGAATGCTAACAGAGCTCCTTCGGGGTGAAGATAAATGAC1330.11055602197820467No Hit
AGTTATGGGACACAAAGCCTCAGCATCAGTCTCACACCTAACGTGATGCTGG1330.11055602197820467No Hit
CTTCAAGGGGCGGCGGAGCTGGCGACGCTGGGCGCCCTCGAGCAGCAGCGAG1330.11055602197820467No Hit
CTACCTGGGGAGGAACTGGGCCTGAAAGGGTACCGGTGACCGCTACTGCTGC1330.11055602197820467No Hit
CTTGGCGGGAAGTATGTGGAATTCTAATTAACTCCTAAAGTGGTCATTTTCA1310.10889352540710384No Hit
GCTACTGGGGAGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTA1310.10889352540710384No Hit
GCATATGGGACCAATTTTGTGAATAAAATGTTTTTATGTATTTCAGATTATT1300.10806227712155345No Hit
AACTCAGGGGTTGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAA1300.10806227712155345No Hit
GTAGTTGGGGAAGTCACAAAGAAAAAGCCTAAGTGAGTGTTTTAATACAGAA1300.10806227712155345No Hit
AATGCTGGGATCCAAAACTGAAACATCTGTCATCTGTATCACATCTAAAGAT1260.1047372839793518No Hit
CAACCAGGGATTGCTAGGTTCATCAGTTATAACTGCCAGCTGTTAGAAGAAC1260.1047372839793518No Hit
TCTTACGGGCGATCTAGTCCAGGGTGGAATGGGTCTCTAGCCTCAGTCTTGT1260.1047372839793518No Hit
TGGTCGGGGGACTGCGTTGAAAGCTTCTGAATTTCAAGCAGAAAGTAGAAGG1260.1047372839793518No Hit
TTACACGGGAACTCTGCAGCCTTCAAAGCTCGAACCAAAGCTAGAAGCAAAT1240.10307478740825098No Hit
AAAGTGGGGACTGTACATCAAAGGATTCATACCGGAGAAAAACCATATGAAT1240.10307478740825098No Hit
ATTAATGGGATGGCGCTCAGAGGCCAAAATTCATCAGCCCCAGTTCTCAGGT1240.10307478740825098No Hit
TTTCGGGGCGGGAGACACGGAGGACTGTTCGGGATTCAGACAGTGGACTGGA1230.10224353912270055No Hit
TAGCCAGGGGAAAATGCGTAAATTGGAAGGCAAGTTCTGAAATTAAACGTTG1230.10224353912270055No Hit
ATGCTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGTTTCTGCTGAGGGGGCA1230.10224353912270055No Hit
CACCTAGGGATTTTAGGTATTGGCAATATTCCAATAAGCATGATAAAAATTG1230.10224353912270055No Hit
TTGCTTGGGACCAGGAAAGCCTTACAAAGAAACAGAGCCGCCCAAGGGGGAA1220.10141229083715014No Hit
CTAGCAGGGCAGTGGTTTTTGTAAAATGCAGGAAAATAAATAATACCCTGCT1220.10141229083715014No Hit
TTCAACGGGCACGTCAAGTACCGCGCCCGCTTCTTCCACCTGGCTGACCTCT1210.10058104255159975No Hit
GAGTTTGGGATCCTGGGTCCTTGCCCTAGTGTGTCAGAAAAACCAGATCACA1210.10058104255159975No Hit
AGCGTTGGGCCTGTGCAGAGGAAAAATGAAGAATTCTCCCAGAAGTGACTTG1210.10058104255159975No Hit
TAACACGGGATTTCAGACTTGCTAAAGATATTAGTATGTGCATTCTATTCAT1210.10058104255159975No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)