FastQCFastQC Report
Sat 31 Dec 2022
SRR5845967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119899
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5400.4503790690497836RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4500.37531589087481965No Hit
CTGACAGGGGGCCGGTCGCCCAGACATGGCGGACGGCAGCGGCTGGCAGCCG1640.13678179134104537No Hit
ACTCTAGGGGAGATAAGTTTTTTTCTCTTTGAAAGATAGAGATTAATACAAC1470.12260319101910774No Hit
CCATGAGGGAGATGAGGGGCAGGCAGTCAGGCAGAAATGGGCAAGTTCAGGG1400.11676494382772167No Hit
GAACACGGGGAAAATTGCATTGATACTAAATATTGTGGCACTTCGGGAAACC1400.11676494382772167No Hit
AGTCAAGGGACACGGACCAGTGTGCAGAAGGCTCCGGGAACCGCAGATTATG1390.11593090851466652No Hit
TGCATTGGGGTACATCAAGCTTTCACTGATTCCCTAAGATTTTTGTTTCCTC1380.11509687320161135No Hit
CTGCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1370.11426283788855619No Hit
TCACGTGGGAGGGGGGCCGTCTGCAGCGCGTGGAGGTGACCTGGCGAGGCTC1350.1125947672624459No Hit
AATTATGGGATGCTGCCTGCAGCCATGAAGGGCCTCGGCCTGGCGCTGCTGG1330.11092669663633559No Hit
CGTGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1320.11009266132328042No Hit
ACCAAAGGGAGTTTGATCCATTTCCATCCATCTGCTCATGAGAGAAGAAATT1310.10925862601022526No Hit
AATTACGGGGTGAGAATGGATACTTATTTTCATTTTGGCCTAAATCATAGGT1310.10925862601022526No Hit
CATCCAGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC1300.10842459069717013No Hit
CTACCTGGGGAGGAACTGGGCCTGAAAGGGTACCGGTGACCGCTACTGCTGC1300.10842459069717013No Hit
TGGTCGGGGGACTGCGTTGAAAGCTTCTGAATTTCAAGCAGAAAGTAGAAGG1280.1067565200710598No Hit
CTTGGCGGGAAGTATGTGGAATTCTAATTAACTCCTAAAGTGGTCATTTTCA1270.10592248475800466No Hit
TTTCGGGGCGGGAGACACGGAGGACTGTTCGGGATTCAGACAGTGGACTGGA1270.10592248475800466No Hit
TAACACGGGATTTCAGACTTGCTAAAGATATTAGTATGTGCATTCTATTCAT1260.1050884494449495No Hit
AGTTATGGGACACAAAGCCTCAGCATCAGTCTCACACCTAACGTGATGCTGG1250.10425441413189433No Hit
TTGTGTGGGGATCTAACCGCGGGTGGACCTAACAGACATTTCCAGAACTTTT1240.1034203788188392No Hit
TTGGTTGGGACTTCACAGGTGTGTAAGTAGGAAGGCAACATTTTCAAAAAGA1240.1034203788188392No Hit
TTGAATGGGGGAGGAAAGAAATGGAAATTCAAATTCAAGAAGCTTAAACAAG1240.1034203788188392No Hit
GGTCTAGGGTACTTTTCTAGAGCTTCTTAAAATTAAATAAATGGTCATTTTT1230.10258634350578404No Hit
CGTCTTGGGCACGGCCCAGCAAAAGCGATGCTGAGAGGGGAAACAGTCCAGA1220.10175230819272887No Hit
TAGTCTGGGGTAGATGTTGTGTGAAATATTTATTTTGATTTTCTAGTTTTAT1220.10175230819272887No Hit
TGTAAAGGGTAGATGATATGTTACTCATTGTAAAGACCACATATTTTTATTC1220.10175230819272887No Hit
TGGGAGGGATTCAGCGGGTGCCAAAGCCTTTGTCTGTGATCAGTGCGGTGCA1210.10091827287967373No Hit
GCCAAGGGAATTATGGCTGATTGGATAGAAATGAAATATCCACTCAGTGGTC1210.10091827287967373No Hit
CACAACGGGTACTGTGATCACCAGCCTACTTCCAATCTCCACCCACCTCCCT1210.10091827287967373No Hit
AGATTGGGGAAAGTGAATTTGATTGAAAAGGCAAATAGCTATTAGGGAAGCA1210.10091827287967373No Hit
TTGCTCGGGGCATCGCGGAGACCAGGCCGGCGGGCGCGCCCGAAGCGCCCAT1200.10008423756661858No Hit
GCTAAGGGGGAGTCATGATCACCGAGACCGCGGCGGAGCCTACGGTCCCTGC1200.10008423756661858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)