FastQCFastQC Report
Sat 31 Dec 2022
SRR5845966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127779
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT8370.6550372126875309RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC7960.6229505630815705No Hit
AGCGAAGGGCTCCGGTCTGTGGACCCTGCCACATTCCACGGCCTGGGCCGCC2330.18234608190704263No Hit
TCTTCAGGGAGTGCACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAA1840.14399862262187058No Hit
TAGCATGGGCCCTCAGAAAAGAGGTAAGTCGGGGGCGAAGGCCCGAGACCCG1750.13695521173275735No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACACTGTCTCTTATACAC1600.12521619358423527No Hit
TACATAGGGAAATGGTATCTTATTTAATTGTCCTGTTCCTTCCCACTCCCCG1560.12208578874462941No Hit
AGCCATGGGACAACTACATCAAATTTTTTCTTGTTTTACCTTAAAAGTGTTA1520.11895538390502353No Hit
GATCAAGGGGTGGCGTCCAGGTGGCAGAGCAGCTAGGAACGCAAGGCCTGAA1510.11817278269512205No Hit
GCTAGGGCTAAAACAAGTTGCTAGGAGGGTATTTCACCGTTGACAGTGCTGA1510.11817278269512205No Hit
TAAACAGGGAAATACAAGAAGCTCAAAGAACACCTGGGAAATTCATCACAAA1500.11739018148522057No Hit
AAGCAGGGGACCAAGGTGGTACCTCTGTGAGTCTGTAGGAGCCACTCCATTA1480.11582497906541764No Hit
TGATGTGGGGAGGTTTATTTCAGTACATTTCCTGCCTCCATCCTTGTGATTC1480.11582497906541764No Hit
TGCTTCGGGAAGCTGCAGTGAGATACCATCTCACACCAGTTAGAATGGTGAT1480.11582497906541764No Hit
TGACTAGGGGGGCTCGCCTGGCCCGGCTGCAGTGGATGTTGCTAGAACCCAC1460.1142597766456147No Hit
CGTAGCGGGTATGTGAGGCTTCCTTGTTACTTTGAAAGCTTTCCTTCTGCAT1450.11347717543571323No Hit
AAATCGGGGATCTCAATGCGCTGCATGGTCCAGTTTGTGGGACGGGAGGCCA1450.11347717543571323No Hit
CTTTGCGGGGAAATTGAAGAAGACACAAATAGAAAGAGATCTCATGCACATG1450.11347717543571323No Hit
GCTGCCGGGAGTCGGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCAG1450.11347717543571323No Hit
ATCGTAGGGATCAGCCAACGCCGGAGACCCCGGGCAGCGTGACTCCAAAAAA1440.11269457422581175No Hit
AAGTGGGGGAACTAATACATGGTGCATGATACTTTCTACTTAGGAATCTTGT1440.11269457422581175No Hit
GGTAATGGGCAGCCAGAAGTCGAAACCTTAAATCAGTCAAATTAAACTTGGG1410.11034677059610734No Hit
CGGTTTGGGACTCTACCGAGTGGTTTTTGTATATACTAAAGTTCTATTTAGA1400.10956416938620588No Hit
GGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1390.1087815681763044No Hit
AAATAAGGGGACTGTGTTCTTTCTATAAAAATCAGGCATTGTGAGAAGCCCA1390.1087815681763044No Hit
AGTCATGGGAGAGTATTCATACTGGTGAGAAACCTTTTGAATGTAAGGAATG1390.1087815681763044No Hit
ACGAATGGGCTCGAGCTCGACGAGTTCGATGACGACGAGTTCTGCGTCGTAC1380.10799896696640293No Hit
GGTGCAGGGCTCTGTGTTTCATGTGTCCCAGGTCCCCCAAAAAACAGGTGGT1370.10721636575650147No Hit
CACGGCGGGTAGAGGGAAATTTATAGCACTAAATGCCCACAGGAGAAAGTGG1370.10721636575650147No Hit
GCCTTCGGGCGATCTTGGTGCGGGGAAGGGTCAAGGAACAGATTCTCTCCTA1370.10721636575650147No Hit
TCTCGCCGGGGGGCCCTCCAGTTCACTATCAGTAGCTTTTGCACTGGTCTGC1360.10643376454659999No Hit
CGATGCGGGAGCTCACTGCAACCTGCACCTCCTGGGTTCAAGTGATTCTCCT1360.10643376454659999No Hit
TGACAAGGGAGTTTAATTTTTTTAAAAAAAATCTGCACTAAAATCCCCAAAC1350.10565116333669852No Hit
AGCTCTGGGCATAAAATTACATTGGCTAATAGCATCACCAATCTCATCAGAG1350.10565116333669852No Hit
GCATTAGGGCATATTTGGGTCTGTCTGGCTCTAAAACTCAAGTTATTTTTAC1340.10486856212679704No Hit
AAATAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1330.10408596091689558No Hit
CGTCATGGGCTGAGCTGTAATTGACTTTAATAAGCTCCAACAGCACTGAAAG1330.10408596091689558No Hit
TGATAAGGGACCTTTTCTCAGGCCTGTGATTCACAGACTTTAACATGAATCA1330.10408596091689558No Hit
ACTCTCGGGCATTTGTCGCTGCCATATCAGTTATTATTGTATCTGAATTGGG1320.1033033597069941No Hit
GACAACGGGAGCTTTGAATGCAGCCCAACACAAATCTGTAAACTTTCTTAAA1320.1033033597069941No Hit
ATATGCGGAGTTTGAGGACAGGTAAAATCAAAACGTGCATGCTCTCTTCAGG1320.1033033597069941No Hit
TTTCGAGGGATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAGG1320.1033033597069941No Hit
CTGGCCGGGGAGAGCTGGTGGGGAGACAGCATGCCACTGGAAAAATTCGTAG1310.10252075849709263No Hit
CGATCCGGGAATATTACTAGGAAAGTTACTTTTCAAAGCAGGAATGTGCTGG1310.10252075849709263No Hit
CGTTTTGGGTGTGAAGAATGTCATTGATAGTTTGATAGCAATAGCATTGAAT1310.10252075849709263No Hit
TTGCAGGGGATCATACAATATGTGGTCCCTTGGGTCTGGCTTTTTTACTTAG1310.10252075849709263No Hit
GGTCCTGGGGGAAAATAATAAGAGTTAGTACTGTTAATAAATATGCCAGTGT1310.10252075849709263No Hit
GGGCTTGGGACTTCGAGCCGGGCCTGCAGGAGGCGCACAAGTGGCGCATGCA1300.10173815728719117No Hit
TGGCAGGAGAACCACTTGAACCCACGAGGTGGAGGTTGTAGTGAGCTGAGAT1300.10173815728719117No Hit
TATCTAGGGGGACTTCTCAAAGTCAGTGCTGTGGTTTTGGGCTGCCTGATTG1300.10173815728719117No Hit
TCAAGCGGGATTTAGTGTTTAGGAATAAAAACATTTGGCTAGTTATCTCTTC1300.10173815728719117No Hit
TAGAATGGGTGGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCT1290.10095555607728969No Hit
CCTTCTGGGAGAGTCCTTTTCCACTCCTCAAAACAGACCACTCGTCCTTGCC1290.10095555607728969No Hit
ATGAGCGGGAAGATGTAGATGGATATAATATTTAGACTTTATATACACCCAT1290.10095555607728969No Hit
AATTAGGGTAGCGCTGTAACCTGAGGGGCCGCCTTGGTTTTGTGGGTGCTCC1280.10017295486738823No Hit
GTAGTGGGGCTGAGCTACATTTTTATTTGTAAAGTGACTCTGTCTGCATGGC1280.10017295486738823No Hit
ATGAAGGGGCTAACAGGCCATGGACCAGATGCCTGTCCTAAACTATTACAGC1280.10017295486738823No Hit
AGGAACGGGAATTTCCACACGCTGTCACATGGAATCTTCAGAGTCACCATGT1280.10017295486738823No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)