FastQCFastQC Report
Sat 31 Dec 2022
SRR5845965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127545
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC7890.6186051981653534No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT7680.6021404210278725RNA PCR Primer, Index 14 (95% over 22bp)
AGCGAAGGGCTCCGGTCTGTGGACCCTGCCACATTCCACGGCCTGGGCCGCC1890.148182994237328No Hit
AAATCGGGGATCTCAATGCGCTGCATGGTCCAGTTTGTGGGACGGGAGGCCA1730.13563840213258066No Hit
TAGCATGGGCCCTCAGAAAAGAGGTAAGTCGGGGGCGAAGGCCCGAGACCCG1660.1301501430867537No Hit
CTTTGCGGGGAAATTGAAGAAGACACAAATAGAAAGAGATCTCATGCACATG1630.12779803206711357No Hit
AGCCATGGGACAACTACATCAAATTTTTTCTTGTTTTACCTTAAAAGTGTTA1570.12309381002783332No Hit
ATCGTAGGGATCAGCCAACGCCGGAGACCCCGGGCAGCGTGACTCCAAAAAA1550.12152573601473989No Hit
TGCTTCGGGAAGCTGCAGTGAGATACCATCTCACACCAGTTAGAATGGTGAT1500.11760555098200634No Hit
GACAACGGGAGCTTTGAATGCAGCCCAACACAAATCTGTAAACTTTCTTAAA1480.11603747696891294No Hit
TCTTCAGGGAGTGCACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAA1480.11603747696891294No Hit
GATCAAGGGGTGGCGTCCAGGTGGCAGAGCAGCTAGGAACGCAAGGCCTGAA1480.11603747696891294No Hit
GCTAGGGCTAAAACAAGTTGCTAGGAGGGTATTTCACCGTTGACAGTGCTGA1480.11603747696891294No Hit
CGATCCGGGAATATTACTAGGAAAGTTACTTTTCAAAGCAGGAATGTGCTGG1440.11290132894272609No Hit
TATCTAGGGGGACTTCTCAAAGTCAGTGCTGTGGTTTTGGGCTGCCTGATTG1440.11290132894272609No Hit
AAATAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1430.11211729193617939No Hit
GGGCTTGGGACTTCGAGCCGGGCCTGCAGGAGGCGCACAAGTGGCGCATGCA1410.11054921792308597No Hit
AGGTTAGGGATATCCTGGACCCTGAGCTGCTGGGGGCATCGGCCCGGCCTGA1400.10976518091653925No Hit
GCTGCCGGGAGTCGGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCAG1400.10976518091653925No Hit
AGTCATGGGAGAGTATTCATACTGGTGAGAAACCTTTTGAATGTAAGGAATG1390.10898114390999254No Hit
AAGCAGGGGACCAAGGTGGTACCTCTGTGAGTCTGTAGGAGCCACTCCATTA1380.10819710690344585No Hit
TAAACAGGGAAATACAAGAAGCTCAAAGAACACCTGGGAAATTCATCACAAA1370.10741306989689914No Hit
GTCCTAGGGGTTCCAAATATGCAGTCAAGGAAATTTTCAATGTGATGAGAGA1350.1058449958838057No Hit
TTGCAGGGGATCATACAATATGTGGTCCCTTGGGTCTGGCTTTTTTACTTAG1350.1058449958838057No Hit
TGATGTGGGGAGGTTTATTTCAGTACATTTCCTGCCTCCATCCTTGTGATTC1350.1058449958838057No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTAC1350.1058449958838057No Hit
AACCTCGGGCAGACTTTAAACCAACAAAGATCAAAAGAGACAAAGAAGGCCA1340.105060958877259No Hit
TGACAAGGGAGTTTAATTTTTTTAAAAAAAATCTGCACTAAAATCCCCAAAC1340.105060958877259No Hit
CGATGCGGGAGCTCACTGCAACCTGCACCTCCTGGGTTCAAGTGATTCTCCT1340.105060958877259No Hit
ATGAAGGGGCTAACAGGCCATGGACCAGATGCCTGTCCTAAACTATTACAGC1340.105060958877259No Hit
CATAGCGGGGGACCACAGGCAGGAACTACCAGGCCCAAAGGAGAGGAAAACA1340.105060958877259No Hit
CGGTTTGGGACTCTACCGAGTGGTTTTTGTATATACTAAAGTTCTATTTAGA1340.105060958877259No Hit
GTTGCCGGGAAATGTACTGGATGTGGTCCCTGCCCTTAAAAAATTTACAGTA1330.1042769218707123No Hit
GCTTAGGGGATATTATGACAATGAGTTCATGGGAATGTGGAAATATTAATCT1330.1042769218707123No Hit
CGTAGCGGGTATGTGAGGCTTCCTTGTTACTTTGAAAGCTTTCCTTCTGCAT1320.1034928848641656No Hit
ACATCAGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAAA1320.1034928848641656No Hit
TGACTAGGGGGGCTCGCCTGGCCCGGCTGCAGTGGATGTTGCTAGAACCCAC1320.1034928848641656No Hit
CGCGGTGGGATCTTTCTAAAGGACTGACGTTCAATCAAATATCTGAAAATAC1310.10270884785761888No Hit
CACTGTGGTTATTACAGCTGAGTGATGGGTATCTGTAGGTTCAATTCACTTT1290.10114077384452545No Hit
GTCTAGGGTCATTTTCAGCATTACCAGTATCACATATGGCAGAGTACTTTAG1280.10035673683797876No Hit
CGCTCAGGGCAGACAATTCTGACATGCATAGATGGACAAAGTTGATTAATGA1280.10035673683797876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)