FastQCFastQC Report
Sat 31 Dec 2022
SRR5845964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123829
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT7860.6347463033699698RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC7520.6072890841402256No Hit
AGCGAAGGGCTCCGGTCTGTGGACCCTGCCACATTCCACGGCCTGGGCCGCC1640.13244070451994283No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACACTGTCTCTTATACAC1610.13001800870555363No Hit
ATATGCGGAGTTTGAGGACAGGTAAAATCAAAACGTGCATGCTCTCTTCAGG1590.1284028781626275No Hit
AGCCATGGGACAACTACATCAAATTTTTTCTTGTTTTACCTTAAAAGTGTTA1590.1284028781626275No Hit
ACATCAGGGAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGCCACTGGAAA1510.12194235599092297No Hit
TGATGTGGGGAGGTTTATTTCAGTACATTTCCTGCCTCCATCCTTGTGATTC1510.12194235599092297No Hit
TACATAGGGAAATGGTATCTTATTTAATTGTCCTGTTCCTTCCCACTCCCCG1490.12032722544799684No Hit
TGCTTCGGGAAGCTGCAGTGAGATACCATCTCACACCAGTTAGAATGGTGAT1490.12032722544799684No Hit
GCTAGGGCTAAAACAAGTTGCTAGGAGGGTATTTCACCGTTGACAGTGCTGA1480.11951966017653376No Hit
ATGAAGGGGCTAACAGGCCATGGACCAGATGCCTGTCCTAAACTATTACAGC1450.11709696436214456No Hit
TCGCCGGGCAGTGTAAGATAATTTAGGGTGAGAATATGGCAAAGTGCCTAGT1440.1162893990906815No Hit
CTTTGCGGGGAAATTGAAGAAGACACAAATAGAAAGAGATCTCATGCACATG1420.11467426854775536No Hit
CGTAGCGGGTATGTGAGGCTTCCTTGTTACTTTGAAAGCTTTCCTTCTGCAT1410.11386670327629231No Hit
ACGAATGGGCTCGAGCTCGACGAGTTCGATGACGACGAGTTCTGCGTCGTAC1410.11386670327629231No Hit
AAGCAGGGGACCAAGGTGGTACCTCTGTGAGTCTGTAGGAGCCACTCCATTA1400.11305913800482924No Hit
TCTTCAGGGAGTGCACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAA1390.11225157273336618No Hit
CTACAAGGGATATACTGCATGTGCATATTATAAAGAAATATCTTTTTTGCAA1380.11144400746190312No Hit
CGATCCGGGAATATTACTAGGAAAGTTACTTTTCAAAGCAGGAATGTGCTGG1350.1090213116475139No Hit
AAATCGGGGATCTCAATGCGCTGCATGGTCCAGTTTGTGGGACGGGAGGCCA1350.1090213116475139No Hit
ATCGTAGGGATCAGCCAACGCCGGAGACCCCGGGCAGCGTGACTCCAAAAAA1340.10821374637605084No Hit
CGATGCGGGAGCTCACTGCAACCTGCACCTCCTGGGTTCAAGTGATTCTCCT1340.10821374637605084No Hit
TGACTAGGGGGGCTCGCCTGGCCCGGCTGCAGTGGATGTTGCTAGAACCCAC1330.10740618110458779No Hit
GCATTAGGGCATATTTGGGTCTGTCTGGCTCTAAAACTCAAGTTATTTTTAC1320.10659861583312472No Hit
TAGAATGGGTGGGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCT1320.10659861583312472No Hit
CTGGCCGGGGAGAGCTGGTGGGGAGACAGCATGCCACTGGAAAAATTCGTAG1320.10659861583312472No Hit
GATCAAGGGGTGGCGTCCAGGTGGCAGAGCAGCTAGGAACGCAAGGCCTGAA1320.10659861583312472No Hit
CTGTGTGGGTGATCACTGCCTACTTGTGATTCAAATCTTCAGAGTTTTCCTA1320.10659861583312472No Hit
CACTGTGGTTATTACAGCTGAGTGATGGGTATCTGTAGGTTCAATTCACTTT1310.10579105056166165No Hit
CGCTCAGGGCAGACAATTCTGACATGCATAGATGGACAAAGTTGATTAATGA1310.10579105056166165No Hit
GAAGTGAGGGGTTGTTTAATCTGGCAAGCGAACAATCTTTGGCAGTTCTTCC1300.10498348529019857No Hit
GTAGTGGGGCTGAGCTACATTTTTATTTGTAAAGTGACTCTGTCTGCATGGC1290.10417592001873552No Hit
GTGTGCGGACTGCGAGCGCCTGTCGGCTCAGATCGAGGAATGCATCTTCCGG1270.10256078947580938No Hit
TTGCAGGGGATCATACAATATGTGGTCCCTTGGGTCTGGCTTTTTTACTTAG1260.10175322420434632No Hit
GTATTCGGGGAGCGGCAGAGGCAGCATCCAGCGGCGGCGCCAGCAGTTCCAG1240.10013809366142018No Hit
TGTGATGGGACGGGCGGGAACAAGCAAATATTTACCTGAGCATATAAAAACT1240.10013809366142018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)