FastQCFastQC Report
Sat 31 Dec 2022
SRR5845960.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845960.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186066
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC5820.3127922350133823No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5250.28215794395537064RNA PCR Primer, Index 14 (95% over 22bp)
TAGCATGGGTCTGACATGAAGAAGATGCTTCATGGCTGTTTTGTTTTCCTTC2440.13113626347640084No Hit
ACTCCCGGGCAGAATCTGCAATGAACTCAAACAAATTTACAAGAAAAAAACA2290.12307460793481882No Hit
GTCTCGGGGATACTGCCTCGCTGACACAAATTACTTATCAGTATGTTTTTTG2270.12199972052927455No Hit
GTAATGGGGAATATGCACCAGTTGTTTTCCCTCTTCAGGAATACATGTTAAT2260.12146227682650242No Hit
CTTCTGGGGGCGTGCCGGCAGGCCGGTCAAGGAGGCGGGACACGTCGGCGCT2250.12092483312373029No Hit
CGTGTTGGGAAGATCTTAGTGAATAATAAGCAACAATACATGTTGACGAATG2200.11823761460986962No Hit
GCAATTGGGGAAATACAAAGCAAAACTTCAATGAAATACTTCATACCCATTT2190.11770017090709749No Hit
TAGCGCGGGTCTACACGGACGACGCCGGCAGGCCCCAGACTGCCTACCTGCA2160.11608783979878108No Hit
CAAACTGGGAACCCATCTGTTCCTGGAGCACTGTCATTCCTCAGAAACTTTA2130.11447550869046468No Hit
TACTATGGGGATCTCCACTAGGGATATCAGGCCCTGGATGTTAATTACAGAA2110.11340062128492041No Hit
GCGCAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2090.11232573387937614No Hit
AATCTGGGGGGCGCGCGCCGAGGGAGGAGCGGGCGCCGGGGGCCGGCTGGCG2090.11232573387937614No Hit
ATCAAGGGGCTGGAGAAGCGTGCCTCAGGCCAGGCTTTTGAGCTGATTCTCA2040.10963851536551547No Hit
GCCAAAGGGTAAGTATGAATGGTGATGCTAATTTCCTATGACATAGGTCAAA2010.10802618425719906No Hit
GTCTCAGGACATACCATGGCCTACAAAACCCCTCATGATCAGACAGGGGTAT2010.10802618425719906No Hit
AATGCTGGGATTTGTATTCTGGTTTTTGTCAATTGACCCAATATTGTTTTTT1960.10533896574333838No Hit
TTCGTGGGGCGATCTACATGTGGGGAGGGCCGAGCGCGGAACGCGAGGGGCT1950.10480152204056625No Hit
CTTGTGCTGGGGATGTTGATTTCATGTATCTGTGAGTGGAGGAGAGTCTGGA1930.10372663463502198No Hit
GGAGATTTGCACTGGGTTAATTAAGGATATGTGCCCTCCAAATGGACTTTGT1930.10372663463502198No Hit
ACAGTTGGGCAAACTAAAGCCTGCAAGCGCCACAATTCTAAGTGCCGCCTTC1930.10372663463502198No Hit
AAGTTGGGGTCTGGCAGAAATATTATACCATTTACCCACATAGATAAAAACA1910.1026517472294777No Hit
AATGCGGGGGAGTCAGAGCGAGAACATTCCAGAGGTCGCCCAGCTCCGGCGC1900.10211430352670557No Hit
GCACTGGGCAGCGAAACAGGTACTATTATTACCCCCTTTTTGTAAATGAGGA1900.10211430352670557No Hit
TGTTAAGGGAGAACCCAAAGATACACAATCAGTTTTGAAATGCAACTTAAGC1890.10157685982393344No Hit
ACTAAGGGATGGATTCTAGAGGGTCTAGGAATCTCTTGAAATTGTCCCTAAA1880.1010394161211613No Hit
GCTCGAGGTCCAATGTGTTTATTCAGAATTTTCTTGAAAGCAAAAAGGAGTA1870.10050197241838917No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)