FastQCFastQC Report
Sat 31 Dec 2022
SRR5845959.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845959.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186148
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC6120.3287706556073662No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5310.2852568923652148RNA PCR Primer, Index 14 (95% over 22bp)
TAGCGCGGGTCTACACGGACGACGCCGGCAGGCCCCAGACTGCCTACCTGCA2530.1359133592625223No Hit
TAGCCAGGGCCGGAATTAAGCAACCAGGAAAAAAGATACTGAGGATGAGGAA2240.12033435760792487No Hit
ACTCCCGGGCAGAATCTGCAATGAACTCAAACAAATTTACAAGAAAAAAACA2220.11925994370071127No Hit
ATCAAGGGGCTGGAGAAGCGTGCCTCAGGCCAGGCTTTTGAGCTGATTCTCA2190.11764832283989084No Hit
TAGCATGGGTCTGACATGAAGAAGATGCTTCATGGCTGTTTTGTTTTCCTTC2180.11711111588628403No Hit
GCAATTGGGGAAATACAAAGCAAAACTTCAATGAAATACTTCATACCCATTT2160.11603670197907043No Hit
CAAACTGGGAACCCATCTGTTCCTGGAGCACTGTCATTCCTCAGAAACTTTA2150.11549949502546361No Hit
TTCTTGGGGTGGCGTGGGGTCTGCGACTGGGAGTTTGGGAAACTTTTTTCTG2120.11388787416464319No Hit
GCGCAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2080.11173904635021596No Hit
CTTGAGCGGGACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCA2080.11173904635021596No Hit
GGCCGAGGGTCTGGCCTCACTGGGGTCTGAAACCAATAAGAAATACAACAAT2060.11066463244300234No Hit
ACAGTTGGGCAAACTAAAGCCTGCAAGCGCCACAATTCTAAGTGCCGCCTTC2050.11012742548939553No Hit
AATGCGGGGGAGTCAGAGCGAGAACATTCCAGAGGTCGCCCAGCTCCGGCGC2020.10851580462857512No Hit
CGTGTTGGGAAGATCTTAGTGAATAATAAGCAACAATACATGTTGACGAATG2020.10851580462857512No Hit
GCGACTGGGAATAACAATAAATACAACAGGGCTAAATGTTAGAGAATTCTTC2000.1074413907213615No Hit
CTTCTGGGGGCGTGCCGGCAGGCCGGTCAAGGAGGCGGGACACGTCGGCGCT2000.1074413907213615No Hit
TGTTAAGGGAGAACCCAAAGATACACAATCAGTTTTGAAATGCAACTTAAGC1990.10690418376775468No Hit
TACTATGGGGATCTCCACTAGGGATATCAGGCCCTGGATGTTAATTACAGAA1990.10690418376775468No Hit
AATGCTGGGATTTGTATTCTGGTTTTTGTCAATTGACCCAATATTGTTTTTT1980.10636697681414788No Hit
ACTAAGGGATGGATTCTAGAGGGTCTAGGAATCTCTTGAAATTGTCCCTAAA1960.10529256290693427No Hit
GTAATGGGGAATATGCACCAGTTGTTTTCCCTCTTCAGGAATACATGTTAAT1960.10529256290693427No Hit
AGTCCAGGGGAGCTGCAGAATTCTTAGCCTGGATTAGAGAAATAAGAGTATG1950.10475535595332747No Hit
CATCCGGGGGAAAATATCACAGTCCTAAATATATATGCACCTAGTGCTGGAG1930.10368094204611385No Hit
CGTGGGGGGATGGCAATCAGTGTTCAAAACCATGACTTCTTGTGTTGACTCC1930.10368094204611385No Hit
AATCTGGGGGGCGCGCGCCGAGGGAGGAGCGGGCGCCGGGGGCCGGCTGGCG1920.10314373509250703No Hit
CAGCACGGGTCTCCGATGGGGGTGCACCCAGTTTCCACCATGATTAAGGGTC1920.10314373509250703No Hit
CTAGCGGGTGTGATATCCAATGAATACTGTAGCTACAGCTATAAGAAAAGGT1910.10260652813890023No Hit
GCACTGGGCAGCGAAACAGGTACTATTATTACCCCCTTTTTGTAAATGAGGA1890.10153211423168662No Hit
GGAGATTTGCACTGGGTTAATTAAGGATATGTGCCCTCCAAATGGACTTTGT1890.10153211423168662No Hit
CATGAAGGGAATCCTCCCTGATAATATTCTTAGGATACACTTCTATAGTGTT1880.1009949072780798No Hit
TGCCATGGGACTACTGCCGGATGGCAAATCCGGGATCTCGGCTCCGAGAGGC1870.100457700324473No Hit
GATGTTGGGAACTCTTGGTGCTTACATTCTTGTATGGGTTTTTTTGTCTAGT1870.100457700324473No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)