FastQCFastQC Report
Sat 31 Dec 2022
SRR5845958.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845958.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183484
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC5530.30138867694185867No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5250.28612849076758734RNA PCR Primer, Index 14 (95% over 22bp)
TAGCGCGGGTCTACACGGACGACGCCGGCAGGCCCCAGACTGCCTACCTGCA2380.12971158248130626No Hit
CGTGTTGGGAAGATCTTAGTGAATAATAAGCAACAATACATGTTGACGAATG2180.11881144949968389No Hit
CAAACTGGGAACCCATCTGTTCCTGGAGCACTGTCATTCCTCAGAAACTTTA2170.11826644285060277No Hit
GCAATTGGGGAAATACAAAGCAAAACTTCAATGAAATACTTCATACCCATTT2160.11772143620152166No Hit
TAGCCAGGGCCGGAATTAAGCAACCAGGAAAAAAGATACTGAGGATGAGGAA2100.11445139630703494No Hit
ACTAAGGGATGGATTCTAGAGGGTCTAGGAATCTCTTGAAATTGTCCCTAAA2080.1133613830088727No Hit
TAGCATGGGTCTGACATGAAGAAGATGCTTCATGGCTGTTTTGTTTTCCTTC2070.1128163763597916No Hit
CTTCTGGGGGCGTGCCGGCAGGCCGGTCAAGGAGGCGGGACACGTCGGCGCT2070.1128163763597916No Hit
TTTGAGGGGAGTGGCACTGGCCACAAGTTAAGGGCTTGATAACTGCAGGTGG2020.110091343114386No Hit
GCTCGAGGTCCAATGTGTTTATTCAGAATTTTCTTGAAAGCAAAAAGGAGTA2010.10954633646530487No Hit
GCACTGGGCAGCGAAACAGGTACTATTATTACCCCCTTTTTGTAAATGAGGA2000.10900132981622376No Hit
GTAATGGGGAATATGCACCAGTTGTTTTCCCTCTTCAGGAATACATGTTAAT1990.10845632316714264No Hit
AATCTGGGGGGCGCGCGCCGAGGGAGGAGCGGGCGCCGGGGGCCGGCTGGCG1970.10736630986898041No Hit
ATTAATGGGTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCTGTCGAGC1940.10573128992173704No Hit
GCCAAAGGGTAAGTATGAATGGTGATGCTAATTTCCTATGACATAGGTCAAA1940.10573128992173704No Hit
CTTGAGCGGGACACTCACAACACCCTAGGCTCACTAAACATTCTACTACTCA1940.10573128992173704No Hit
GGCCGAGGGTCTGGCCTCACTGGGGTCTGAAACCAATAAGAAATACAACAAT1920.10464127662357481No Hit
GCGCAAGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1910.10409626997449367No Hit
GTCTCGGGGATACTGCCTCGCTGACACAAATTACTTATCAGTATGTTTTTTG1900.10355126332541258No Hit
ACCAAGGGGATGTTATTATTATTCCCATTTTATAGATGAAGAAACTGAGACA1880.10246125002725034No Hit
TTCTTGGGGTGGCGTGGGGTCTGCGACTGGGAGTTTGGGAAACTTTTTTCTG1880.10246125002725034No Hit
TGTTAAGGGAGAACCCAAAGATACACAATCAGTTTTGAAATGCAACTTAAGC1860.1013712367290881No Hit
CAGCCTGGGCAGGACAGTTAGACACAAGTCTGGGGTTCAGGGAAGATGTTGG1860.1013712367290881No Hit
GGCTATGGGGATCCTGAGGTAACGGATAAGTTTATACCATGGATAGCACAAA1850.10082623008000698No Hit
TAGCAGGGAGTCTGCAGCCGGAGTAAGATGGCGGCGCTGAGGGCTTTGTGCG1840.10028122343092585No Hit
AATGCTGGGATTTGTATTCTGGTTTTTGTCAATTGACCCAATATTGTTTTTT1840.10028122343092585No Hit
ACAGTTGGGCAAACTAAAGCCTGCAAGCGCCACAATTCTAAGTGCCGCCTTC1840.10028122343092585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)