FastQCFastQC Report
Sat 31 Dec 2022
SRR5845957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154136
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC6440.41781284060829393No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5460.3542326257331188RNA PCR Primer, Index 14 (95% over 22bp)
AGCTTTGGGATGAAAGAAAGGATTGTCAATGTAGAATTCTATATTTTCAAAA2000.12975554056158198No Hit
GTCTGAGGGCTTTACCATTTTTGTAGGGTCTGCAAAGATGCTTTGAGGAAGG1650.10704832096330513No Hit
CGAAGCGGGCGATCTAAGTTGGGGGGCCCACAGCTGTTTCTCTCGCCCCCAG1630.10575076555768932No Hit
ACTAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1600.10380443244926557No Hit
AGACCTGGGAGGAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTC1590.10315565474645769No Hit
GAAATGGGGATGGAAAATTTACAAACAGAGATTCAAGAAATGGAATTCCTTC1560.10120932163803394No Hit
AGCTTAGGGCATAATCATCAGAGAGAAAGGTCGGGTTACCCACAAAGGGAAG1550.10056054393522604No Hit
TGGAGTCGGGGATATCACCAGTTTTGTTGTTATTAGTAATTTCTAACTGCCC1550.10056054393522604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)