FastQCFastQC Report
Sat 31 Dec 2022
SRR5845956.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845956.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154656
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC6020.3892509828264018No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5350.34592902958824745RNA PCR Primer, Index 14 (95% over 22bp)
AGCTTTGGGATGAAAGAAAGGATTGTCAATGTAGAATTCTATATTTTCAAAA2380.15388992344299607No Hit
GTCTGAGGGCTTTACCATTTTTGTAGGGTCTGCAAAGATGCTTTGAGGAAGG2230.1441909786881854No Hit
CGAAGCGGGCGATCTAAGTTGGGGGGCCCACAGCTGTTTCTCTCGCCCCCAG1720.1112145665218291No Hit
TGGAGTCGGGGATATCACCAGTTTTGTTGTTATTAGTAATTTCTAACTGCCC1640.10604179598593007No Hit
TGCTAGTGGGTAAGAAGGAGCTATGATAAAATGCTAGCCACCATGAGCTCCT1620.10474860335195531No Hit
AGACCTGGGAGGAAAAAGCAGATTGAAGAGCTGAAGGGACAAGAAGTTAGTC1580.1021622180840058No Hit
AAGTATGGGCCTTGATGGAATTAAAACAGTCTCCACTTTCTATAAAATCCCT1580.1021622180840058No Hit
AAAACAGGGACATGGGGTTATTTCAAAGAAAAGAAAATTAAAAACACCTTGC1570.10151562176701841No Hit
TGCACAGGGGGCATCAGAAAGGCATTTACAGTAAATGGCTACCCCCCCTTCC1560.10086902545003104No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)