FastQCFastQC Report
Sat 31 Dec 2022
SRR5845955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152368
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC5500.3609681822954951No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT5420.3557177360075606RNA PCR Primer, Index 14 (95% over 22bp)
AGCTTTGGGATGAAAGAAAGGATTGTCAATGTAGAATTCTATATTTTCAAAA1980.12994854562637823No Hit
CAGCCTGGGAGACAGAGTGAGACCTTGAGACTTTGCATCAGAAACAAAACAA1760.11550981833455844No Hit
CTGCGGGGAAACTGCAGAAATTGTGGTTTTGGTTCAGAAACAAGTTGTATAT1740.1141972067625748No Hit
GTCTGAGGGCTTTACCATTTTTGTAGGGTCTGCAAAGATGCTTTGAGGAAGG1740.1141972067625748No Hit
AACTATGGGCAGATGGAAGTCCCGGAGTTTGTGGCCAAAGTGAATGACACCC1710.11222828940459939No Hit
CGAAGCGGGCGATCTAAGTTGGGGGGCCCACAGCTGTTTCTCTCGCCCCCAG1570.10304000840071406No Hit
GCCTCGGCCTCCCGGGGTGCTGGAACTGCAGGCGTGAACCACCGCACCCAGC1560.10238370261472225No Hit
CCGGGTGGGGAAGAAATTATGAAAATTCCGATGAGTTTTTAAAGTTTGTGCT1540.10107109104273865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)