FastQCFastQC Report
Sat 31 Dec 2022
SRR5845953.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845953.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138339
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4630.33468508518928136No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT4600.3325164993241241RNA PCR Primer, Index 14 (95% over 22bp)
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1930.13951235732512163No Hit
GGAGCAGGGACTAATTATCAGAAAAATGCAAACCAAAATCACAGTGAGATAT1840.13300659972964962No Hit
GCTGCAGAGCGAGCCCAGCCTCACCAGCGACGAGCTGCACCTGCCCGGGAAG1750.12650084213417764No Hit
TCCGCCGGGACTAGAAGAAGACAATAAAATGTTGGTGAATGAATTGAATCAT1750.12650084213417764No Hit
CTGGGCGGGATGTTCACTTACAAATATTCATAAATAAGTGTTCACTTACAAA1630.1178264986735483No Hit
GCACTAGGGGTCTTTTCTTTTTCTAGTGGCAGTAATTTAGTGGATGTTTCAT1550.11204360303312877No Hit
AAACCAGGGGTTATTGCTCTTTGTGTTGCCTGCAGTATTTTTCTTCTCATTT1480.10698356934776165No Hit
TGCTAAGGGGTATGAGTCCCATGTGATAAACAGGGCTAGGCAAAATAGCATG1460.10553784543765676No Hit
ATCAGCGGGAGGGAAAGCTATGTAGGTGGTAAAACTAAAAGGCAAAGGAATG1460.10553784543765676No Hit
ACCCCGGGTAAAATGTTATGGTAAGCATGCACAGTTTGCAGTCTACAGTTTT1440.10409212152755187No Hit
TATCGCAGGGGTTGGAGAGTGTAAGCAATAGTTGTGGTGGAGGTTCATGGTG1440.10409212152755187No Hit
ACTACCGGGAGGTAGCGGTAGCGATGGACACTCTGGATCGAGTAGTAAAGCC1430.10336925957249944No Hit
AACGATGGGATGGATTTGAATAAAGAATATGGTTTTATTTTTAAGTAAAATG1430.10336925957249944No Hit
AGCTCTGGGATTTAACTTAAGTTTTATTCAAAATCTCAAACTGTATCATAGC1420.10264639761744701No Hit
ATTACAGGGGCCCGCCGCACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGT1420.10264639761744701No Hit
AGACATGGGTTCAAGAGTTTATCTAGAATAGACACCTAGACATGTAGTTATT1410.10192353566239455No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)