FastQCFastQC Report
Sat 31 Dec 2022
SRR5845952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136060
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGCCGTCT4710.3461708069969131RNA PCR Primer, Index 14 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGTTACATCTCGTATGC4560.33514625900338085No Hit
GAGTCATCTGACACTGATGATGAAGAATTACGGCATCGAATCCGGCAAAAAC1820.13376451565485814No Hit
AGACATGGGTTCAAGAGTTTATCTAGAATAGACACCTAGACATGTAGTTATT1650.12127002792885493No Hit
TGCTAAGGGGTATGAGTCCCATGTGATAAACAGGGCTAGGCAAAATAGCATG1540.11318535940026458No Hit
GCACTAGGGGTCTTTTCTTTTTCTAGTGGCAGTAATTTAGTGGATGTTTCAT1510.11098044980155813No Hit
GGCGTCGGGAGGGTTGTCCAGTGTTTCCTGAAAATGCTTTTGTGCTTTGATA1480.10877554020285167No Hit
ACTACCGGGAGGTAGCGGTAGCGATGGACACTCTGGATCGAGTAGTAAAGCC1450.10657063060414522No Hit
ATGCGGGCATTCTTGTAGAGTGTTCTTGAAACACTCTCTGCACCATTTTTAA1440.10583566073790975No Hit
TCCGCCGGGACTAGAAGAAGACAATAAAATGTTGGTGAATGAATTGAATCAT1440.10583566073790975No Hit
CTGGGCGGGATGTTCACTTACAAATATTCATAAATAAGTGTTCACTTACAAA1420.10436572100543878No Hit
GCTGCAGAGCGAGCCCAGCCTCACCAGCGACGAGCTGCACCTGCCCGGGAAG1400.1028957812729678No Hit
CAAACCGGGCGTCAGGAGCGGGCGCGGGCCGAGGCGCAGGAGGCTGAGGATC1400.1028957812729678No Hit
GCTCAGGGTAGTTCTTTCTTTCTCCCTCCCAGCCTCCTCCCCGTTTACCTTC1380.10142584154049683No Hit
AGCTCTGGGATTTAACTTAAGTTTTATTCAAAATCTCAAACTGTATCATAGC1370.10069087167426136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)