FastQCFastQC Report
Sat 31 Dec 2022
SRR5845894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209263
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC9180.43868242355313647No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT7030.3359408973397113No Hit
CAGTAGGGGAAGTAATCACTTATTTTTTATGTCCATAATTTTATATTGTCTT3420.16343070681391358No Hit
TTAGCTGGGTCCAAAATATAAAAAGATAAAAAGTCATAAAATCATAAATTAA3000.14336026913501193No Hit
TTGACCAGGGATTTGTTGAGGGCCTACTATGTGCCAGGCACTGGAAATAAAG2980.14240453400744518No Hit
TCATACGGGGGTCAAGGCAGCCATCTCGCCGTGAGACAGCAAGTGTCGGATC2900.1385815934971782No Hit
TCGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2880.13762585836961144No Hit
AGTGCTGGGACTGGAAAAAATTAGATGCAATGATATTGACTTAACATGAAAA2870.13714799080582807No Hit
ATCTGAGGGACAAAAACAATAGAGAAAATCAATGGAAGAAGAAAGTGGCTAC2810.13428078542312782No Hit
TTGAAAGGGAGTAATTTCAGAATTATCTAGTGGTAATGGTACTTGCAGGATG2710.12950210978529408No Hit
GCAACTGGGATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACTT2700.12902424222151074No Hit
TCTGGTGGGAAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTG2650.12663490440259387No Hit
GGCAGCAGACTTCTCAGTGGAAACCTTATAGGCCAGGAGAGAGTGGCATGAC2590.12376769901989364No Hit
ACAACAGGGAGGAAAGATCGAAATTTGACACCCTAACATCACAATTATAAGA2590.12376769901989364No Hit
ATGCCGGGGCAATTTCAGACACTTTCCAGAGTCTCTGAAGCGCACTCCTAGG2570.12281196389232689No Hit
TAGCTAGGGACCTTTTGGAAAACATCAACATTTTAACCATAGACCCTTCAGC2560.1223340963285435No Hit
TGTGGGGGGTAAAGAGCCAGAGTGGGAACTGTTGATCCAAGTGTAGCCTGAA2550.12185622876476013No Hit
ACTAATGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG2540.12137836120097677No Hit
GTCATTGGGCAGCAGCTGGAGATGAACCTCAGGAATTTTGCCCAAATCATTG2530.12090049363719338No Hit
GGTTACGGGAGGCCCAAGATAGGGAAGCACTGCTGGTTTACATTTCTGCTCT2530.12090049363719338No Hit
CTCATTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG2500.11946689094584327No Hit
TGTACTGGGGTAATCCTTTTCTGCACACTCGGGTAATTTCTTCTTTTTCATT2500.11946689094584327No Hit
CGAATGGGGGACAGCCTTTTTGTTCAGATTGGCAGTCTCTTTCGCTTATCTG2490.11898902338205991No Hit
GCCTCAGGGTAGCAAGATGTGCCATTGGGGAAAACTGGGTAAAAAGAACACA2490.11898902338205991No Hit
GCTACGGGAGAAAAGTAAGATGGAAGAGGGAAATTTTGCCTGGAAGCCTGGA2480.11851115581827652No Hit
TAGCATGGGAAGCTTGTGAAGCAGATAATCATATCAGAAATTATCAGTTCAT2470.11803328825449315No Hit
GTTGGTGGGCCCAAAATCTGATAATCATGTCTAACCAAGTAATAATGTTTTA2470.11803328825449315No Hit
AACCAGGGGGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCC2460.11755542069070976No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2460.11755542069070976No Hit
TATTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2460.11755542069070976No Hit
GGCATAGGGATTGTAAAAGAAAAGCCTGAAGAAATAAGTCTACAACGATTTA2450.1170775531269264No Hit
TGTTATGGGATTATTCATTTTATGAAGACAATTTTCTTCTAAAGCTAGATCC2430.11612181799935965No Hit
ATGAGAGGGATGAGGTGGCCCCTGAGAGGTGGGGGCCTCTCCAGGGCACATC2430.11612181799935965No Hit
CAGCTTGGGAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTCTT2420.11564395043557629No Hit
TAGACTGGGAATTAAGCCATAATCTCAAAGTGGAAAAATATATGTGGTTATT2410.1151660828717929No Hit
TGAAATGGGAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTG2410.1151660828717929No Hit
TAAATGGGGTCACTAAATACTGGAATATAAAAAATTTGACAAAATATTTTCA2410.1151660828717929No Hit
GTGTTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2400.11468821530800953No Hit
TGTTCAGGGTATTTTTGATTTATTAGCCTTATTTAAGATTTTGTTTCTTTTC2390.11421034774422617No Hit
ACCTGCCTCGGCCTCCCAGGGTGCTGGAATTACAGGCGTGAGCCACCGTGCC2390.11421034774422617No Hit
CACGTAGGGCTACAAAATTAACCAAAAACTCTGACATATTAATGTGGTATTA2380.11373248018044278No Hit
TTATAAGGGATTTTCTTCGTGGGGTAAACAATGGCTAATGATCAGGCTTTCC2370.11325461261665942No Hit
TCAGAAGGGACTGATAAATCTGTTGACACAAGTCCTGCAAAGACCTGAGAGG2360.11277674505287603No Hit
AGCCTCGGGGTTTCGGGGGTTGCAAGATGGCGGCCACCAGTGGAACTGATGA2360.11277674505287603No Hit
TTCCATGGGACCACAAAGTTACTTCTGGATAAGAGCAACCCCAAGATACTAA2350.11229887748909267No Hit
GACATAAGGGGATCTATTGTATTTGAAACATAACTTTGACAATTATTAGTGT2320.11086527479774255No Hit
CAACCAGGGTCTATCTACAGGGGCAACCACTATTCTACTCTCTACTTCTATG2320.11086527479774255No Hit
GCTATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG2310.11038740723395919No Hit
GCAACTGGGAATGAAAGAAAACTACAAAAATAATACTTCTTTTCCACCCGTC2300.1099095396701758No Hit
TCTTAAGGGGGAGTTCAGAGAAAAAAATCACTGTTAGAGATATACATGTAGG2290.10943167210639244No Hit
CGCTCGGGGAAGTTGGTGGAGAGCATGCATAATCTCTGCCAGTGTACAGACT2290.10943167210639244No Hit
AGAATGGGGAAGTGGAAAATGACTTGATACAGGTCTGTAGGCCTCTATGGAG2290.10943167210639244No Hit
TTTGTAGGGATCTGTAAAGATCGAGCTTGTGTGTGGTGTCGTGGTCACATCT2290.10943167210639244No Hit
CGCTCAGGGGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAA2290.10943167210639244No Hit
TATATGGGGGATCTATTGAAGGGAACTCATTGTATTTTAACATCTCTTCCAC2270.1084759369788257No Hit
GTTGCTGGGGGGTTTCGCTGTGTTGACCAGGCTGGTCTCAAACTCCTGACCT2270.1084759369788257No Hit
TCTTGTGGGGAAGTTGCTTTTACGATATTATTTTGGTGGCTTTCTTTTCTCT2260.10799806941504232No Hit
AACTGTGGGAGACACAAATCGAGTATCTAGCTTGGCTTTCTGCACTGAGCAG2260.10799806941504232No Hit
GTTTACGGGGGAGCGGAGAGGGTTATCATTTAACATGGAAGAAGATGAGTTC2240.10704233428747557No Hit
TAATTAGGGACAAACAAGATACGTATATTTTTAACTGCTTCAAGTGTTACCT2230.10656446672369219No Hit
GGCGCAGGGAAGGAGTAAACTATGGTGTCTGTGTAATGGTAAGAGATGGAGT2230.10656446672369219No Hit
CTTTTACGGGGTGATTTAATCTGTTAATATGAATATCTCATTTAAAACTTTT2220.10608659915990883No Hit
TCAGCTAGGGAAGCAGTGAAATGTTCATTTTGTCCAGCTATTCTGTGTCATT2220.10608659915990883No Hit
CAAAAAAAAGGGAAAAAAAAGAGGGAATAGATAATAATATTAAGTACTGGTA2220.10608659915990883No Hit
CACATTGGGCTCTTGTAACAGATTGGTGTGTATTTCTGTGTATCTCTTGTAA2220.10608659915990883No Hit
TAACATGGGCACGACCTCTTCCGATCTTGCTACGGGACAAGTCCTAACCATG2220.10608659915990883No Hit
AGGCCTGGGATGGATGTCTGTAATATACACACACATATACATTTGAAAATGA2210.10560873159612547No Hit
TCTTGCGGGGAAATACTTTTTTAATGCAGATAAAAATTATCAGGAAATTAAC2210.10560873159612547No Hit
ACTGCGGGGAAAGGTATATATTACTCTAGATTTTTCTTACTGTAAATATTGT2210.10560873159612547No Hit
GTCTGCGGGCATGAAATTTCTCTGTGACATTTGCAGCTTTTGCTCTAAGGAT2190.1046529964685587No Hit
TCATAGGGGCAATTTCTTTTAAAGCTAAACACACACATACAGTTAGTTAGGC2190.1046529964685587No Hit
GTGCCAGGGGCACTTTTAAAAAAATAATTGGTTGACTTAATATATTTGCTTT2190.1046529964685587No Hit
TACCAAGGGGTGTGAGATGAGGGTCTCAATTTTTATCTCTGCATGTGGATAT2190.1046529964685587No Hit
TGCAAGGGACATACAGGAGAACCTGGAGCTGGTTGAAAAAGGCTTCAGTAAC2180.10417512890477532No Hit
TCGAACGGGGTCCAGGCTCAGCAGCAGCAGCTCCTCCGAGTCTGGGAGCAGC2180.10417512890477532No Hit
CGTCTAGGGCAGGCCAGCAAGCAATCGGCTTAAAATTCCCTTTCTTAAATGT2180.10417512890477532No Hit
GTTCATGGGAGTGGGAGACTTTAACATCCCATTCTCAGTATTAGACAGATCT2180.10417512890477532No Hit
ACCAATGGGAAGTGGTTCCTGTGGGTTCCTGATTTGCTTACTCATGAGACCA2170.10369726134099196No Hit
CCAAAGGGGTATGTCCCGGGATTTTTATGTCCAATAAATGCTTATTGATTTC2170.10369726134099196No Hit
TCAAATGGGTTGCTACAAAGAGAATAAAATACCTAGGAATACAGCTTACAAG2170.10369726134099196No Hit
TGCCTGGTAAAGAGGTAGAACTGGTTAGATACAGGGGTTTGTTTTGTTCAGT2170.10369726134099196No Hit
ATTGCTGGGATTGTAGGCGTGAGCCACTGCGCCCGGCTGATTTTAAGAGATT2170.10369726134099196No Hit
ACAACAGGGGCCCTGTGAGCGTCTACAGACAGCTCACCATTTTTGTCCTGTA2170.10369726134099196No Hit
TCACCCGGGATAGAAATGGTACTGATTTTTGTATCCTGCAACTTCAGTGAAT2170.10369726134099196No Hit
CCGCTTGGGAAGGATGGGGCTCCAAGGAGATCTCTGAACTTGGAAGACTACA2170.10369726134099196No Hit
TTTTGCGGGAGGAATGTTTTGATGTTTCGTACAATAATTCAAGTTGCAAGAA2160.1032193937772086No Hit
CTTGCTGGGCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA2160.1032193937772086No Hit
TATGCAGGGGTGTTATTTTATTTTGAAGAGTTGTTTATTATATTGTTTCTGG2160.1032193937772086No Hit
CATGTTGGGGAGGAACCCGAGGCACAGAGAAGTAAAATAATTTTCACAAGGT2150.10274152621342521No Hit
GAGTGTGGGCCATTTCACAACTATCAGGTACTCTTCTGGGTACTCATCATGA2140.10226365864964185No Hit
CGTAGCGGGATGCTCCAAAATGTTCACTAAAATGGGATAAACATAAGGCATT2140.10226365864964185No Hit
CAGCTTGGGAGGCCTGCCGTCGTAGGGAGGCTTGCGGAGCAGGAGCGCCCTC2140.10226365864964185No Hit
TCTTGCGGGATGTCTATCAATGAATGAATGGATGAAAAACCATGTGATATAC2130.10178579108585846No Hit
AATAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2130.10178579108585846No Hit
GTCTAGGGGGATTCATTCTTAGAGGTTGTCACTTCTATTCAGAATTGCCTTC2130.10178579108585846No Hit
CATCAGGGAGCCCCAGCCCCGACAGGAAATGGGTCGCTCCCCTGTGGACAGT2130.10178579108585846No Hit
GAGAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC2120.1013079235220751No Hit
TAATTTGGGCAACGTAAGTGCTAAGATGTGATCATTATTTTTGAAGGAACTG2120.1013079235220751No Hit
TTTCAAGGGATCTGGTATTAATTTAATCTCAGGAAAAACAAGAAATTAACCC2120.1013079235220751No Hit
TCATGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2120.1013079235220751No Hit
CCTTGTGGGGTGGGATTGGTGTAGGGTTAAATCATCAACATTATTTCATAAA2120.1013079235220751No Hit
GAAGTAGGGATAACAGGACCCGGGCCCTGCAAAGCCACAGCTCCCCAGAGGG2110.10083005595829173No Hit
CTGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2110.10083005595829173No Hit
CGACACGGGCCTGCCCGCTTCCTTGGGCTCCCCGGCCCTGGGTGGGCGGTGC2110.10083005595829173No Hit
TTATGCGGGATACAATCAATGCTATTTATTGTACCTCTGGGCCTACTCTTCT2110.10083005595829173No Hit
TCTTGAGGGATAACTTGGAAATATTTAAGGAAATCATCAGTTGCCTTAAGCA2100.10035218839450834No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2100.10035218839450834No Hit
CTCACATGGGATTCAGGGCTGATGCATGCCAGGTGAACAATAAATACAGATG2100.10035218839450834No Hit
GGTTGTGGGATGAGTGAAACAAATATTCTCATTGAATATGATACAATGCTGT2100.10035218839450834No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)