FastQCFastQC Report
Sat 31 Dec 2022
SRR5845893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204930
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC8110.3957448884985117No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT6710.32742887815351585No Hit
CAGTAGGGGAAGTAATCACTTATTTTTTATGTCCATAATTTTATATTGTCTT3370.1644463963304543No Hit
TTAGCTGGGTCCAAAATATAAAAAGATAAAAAGTCATAAAATCATAAATTAA3170.15468696628116918No Hit
AGTGCTGGGACTGGAAAAAATTAGATGCAATGATATTGACTTAACATGAAAA2860.13955984970477725No Hit
TAGCTAGGGACCTTTTGGAAAACATCAACATTTTAACCATAGACCCTTCAGC2790.13614404918752745No Hit
TTGAAAGGGAGTAATTTCAGAATTATCTAGTGGTAATGGTACTTGCAGGATG2770.13516810618259895No Hit
GGTTACGGGAGGCCCAAGATAGGGAAGCACTGCTGGTTTACATTTCTGCTCT2740.13370419167520617No Hit
ATCTGAGGGACAAAAACAATAGAGAAAATCAATGGAAGAAGAAAGTGGCTAC2730.1332162201727419No Hit
GGCAGCAGACTTCTCAGTGGAAACCTTATAGGCCAGGAGAGAGTGGCATGAC2720.13272824867027766No Hit
TTGACCAGGGATTTGTTGAGGGCCTACTATGTGCCAGGCACTGGAAATAAAG2700.13175230566534915No Hit
TCGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2660.1298004196554921No Hit
GCAACTGGGATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACTT2660.1298004196554921No Hit
TGTTCAGGGTATTTTTGATTTATTAGCCTTATTTAAGATTTTGTTTCTTTTC2580.12589664763577807No Hit
TAATTTGGGCAACGTAAGTGCTAAGATGTGATCATTATTTTTGAAGGAACTG2570.12540867613331383No Hit
CACGTAGGGCTACAAAATTAACCAAAAACTCTGACATATTAATGTGGTATTA2570.12540867613331383No Hit
TGCCTGGTAAAGAGGTAGAACTGGTTAGATACAGGGGTTTGTTTTGTTCAGT2530.12345679012345678No Hit
TGTGGGGGGTAAAGAGCCAGAGTGGGAACTGTTGATCCAAGTGTAGCCTGAA2520.12296881862099253No Hit
TGTACTGGGGTAATCCTTTTCTGCACACTCGGGTAATTTCTTCTTTTTCATT2510.12248084711852829No Hit
AACTGTGGGAGACACAAATCGAGTATCTAGCTTGGCTTTCTGCACTGAGCAG2460.120040989606207No Hit
CGCTCGGGGAAGTTGGTGGAGAGCATGCATAATCTCTGCCAGTGTACAGACT2460.120040989606207No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2450.11955301810374275No Hit
TGTTATGGGATTATTCATTTTATGAAGACAATTTTCTTCTAAAGCTAGATCC2450.11955301810374275No Hit
TGCAAGGGACATACAGGAGAACCTGGAGCTGGTTGAAAAAGGCTTCAGTAAC2440.11906504660127849No Hit
CATGTTGGGGAGGAACCCGAGGCACAGAGAAGTAAAATAATTTTCACAAGGT2440.11906504660127849No Hit
CGACACGGGCCTGCCCGCTTCCTTGGGCTCCCCGGCCCTGGGTGGGCGGTGC2430.11857707509881424No Hit
ACTAATGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG2420.11808910359634997No Hit
ATTCATGGGATGCCAGTAAATCTTCTCAAAAAAGAATAATGAGGCCCATTTG2400.11711316059142146No Hit
CAAAAAAAAGGGAAAAAAAAGAGGGAATAGATAATAATATTAAGTACTGGTA2400.11711316059142146No Hit
TAATTAGGGACAAACAAGATACGTATATTTTTAACTGCTTCAAGTGTTACCT2390.1166251890889572No Hit
TATTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2380.11613721758649295No Hit
GGCATAGGGATTGTAAAAGAAAAGCCTGAAGAAATAAGTCTACAACGATTTA2380.11613721758649295No Hit
TCTTGCGGGATGTCTATCAATGAATGAATGGATGAAAAACCATGTGATATAC2360.11516127458156443No Hit
GAGTGTGGGCCATTTCACAACTATCAGGTACTCTTCTGGGTACTCATCATGA2350.11467330307910018No Hit
GTCATTGGGCAGCAGCTGGAGATGAACCTCAGGAATTTTGCCCAAATCATTG2330.11369736007417167No Hit
CGTCTAGGGCAGGCCAGCAAGCAATCGGCTTAAAATTCCCTTTCTTAAATGT2330.11369736007417167No Hit
ACAGCAGGGATTAACGAGGCTGTAGAAGTACTGAACAACACCATCAGCCAAC2320.11320938857170741No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2290.11174547406431463No Hit
CAGTGCGGGCGGCTGGGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGG2290.11174547406431463No Hit
GTGTTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2280.11125750256185038No Hit
CGAATGGGGGACAGCCTTTTTGTTCAGATTGGCAGTCTCTTTCGCTTATCTG2280.11125750256185038No Hit
CTCATTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG2270.11076953105938613No Hit
GTTCATGGGAGTGGGAGACTTTAACATCCCATTCTCAGTATTAGACAGATCT2270.11076953105938613No Hit
AGTTAGGGGCTTCTGCTCTGAACCACTGTCGGTGCATCCATATTAATAAAAG2250.10979358805445763No Hit
GTTTACGGGGGAGCGGAGAGGGTTATCATTTAACATGGAAGAAGATGAGTTC2250.10979358805445763No Hit
TCATGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2250.10979358805445763No Hit
ACAACAGGGAGGAAAGATCGAAATTTGACACCCTAACATCACAATTATAAGA2250.10979358805445763No Hit
TAGACTGGGAATTAAGCCATAATCTCAAAGTGGAAAAATATATGTGGTTATT2240.10930561655199338No Hit
CTTGCTGGGCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA2240.10930561655199338No Hit
CATCAGGGAGCCCCAGCCCCGACAGGAAATGGGTCGCTCCCCTGTGGACAGT2240.10930561655199338No Hit
AAGTACGGGGAGAGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGG2230.1088176450495291No Hit
GTCTGCGGGCATGAAATTTCTCTGTGACATTTGCAGCTTTTGCTCTAAGGAT2220.10832967354706484No Hit
CCAAAGGGGTATGTCCCGGGATTTTTATGTCCAATAAATGCTTATTGATTTC2220.10832967354706484No Hit
AGCTCAGGGGCCGCCCGCGCCGCGCCGGAGTCCGCTGTCCGCCAGCTACCCG2220.10832967354706484No Hit
CTTGGTGGGACAAGAATGAAATTAAATGATAATATTCACATTAAGTAGAGTA2220.10832967354706484No Hit
ATGAGAGGGATGAGGTGGCCCCTGAGAGGTGGGGGCCTCTCCAGGGCACATC2220.10832967354706484No Hit
TGAAATGGGAGGGTGAGCATCAAACTCAAACTACGCCCTGATCGGCGCACTG2220.10832967354706484No Hit
AGTACAGGGGCATTTCACGAAGTTTGATGTCTTGTTCTTCCATTGTTTCATC2220.10832967354706484No Hit
CGCTCAGGGGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAA2220.10832967354706484No Hit
AGCCTCGGGGTTTCGGGGGTTGCAAGATGGCGGCCACCAGTGGAACTGATGA2220.10832967354706484No Hit
TTCCATGGGACCACAAAGTTACTTCTGGATAAGAGCAACCCCAAGATACTAA2210.10784170204460058No Hit
TAAACGGGGGGGAGGAGTGATGTTCATTTTGGGATTTGCAGGGTGCATTGGA2200.10735373054213634No Hit
TAACCCGGGCAGCCTCCCGAGTAGCTGGGATTACAGATGTGAGCCACAGCAC2200.10735373054213634No Hit
ACATTGGGGATAAAATTTAAGTAGTAAATTAATTCCCTGCTATGATTATAAG2200.10735373054213634No Hit
GTTGGTGGGCCCAAAATCTGATAATCATGTCTAACCAAGTAATAATGTTTTA2200.10735373054213634No Hit
TTGCTTGGGGGTTAATCATTAAAATGACAGAAAAATTATAATTCTGTATGCA2190.10686575903967209No Hit
AAATATGGGGCACCTGGGAACTCATCTGCTGCCTCTTGGTTGGCAGAAGCTG2190.10686575903967209No Hit
GTGTGAGGGATGAGCAAAGGTATTTCTAAAATGGCTCTTCTATGAAAAGCAA2190.10686575903967209No Hit
CCACCTGGGAACCATTGTGGAAGTCAGTGTGGCGATTCCTCAGGGATCTAGA2190.10686575903967209No Hit
TAAATGGGGTCACTAAATACTGGAATATAAAAAATTTGACAAAATATTTTCA2190.10686575903967209No Hit
GTAGCCGGGAAGGATGTTGGGCTGAAGTTTTCTTCTTTTGTTGTGTCTCTGC2170.10588981603474358No Hit
TTATAAGGGATTTTCTTCGTGGGGTAAACAATGGCTAATGATCAGGCTTTCC2170.10588981603474358No Hit
CGTAGCGGGATGCTCCAAAATGTTCACTAAAATGGGATAAACATAAGGCATT2170.10588981603474358No Hit
TCTGGTGGGAAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTG2170.10588981603474358No Hit
AACCAGGGGGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCC2160.10540184453227931No Hit
TATATGGGGGATCTATTGAAGGGAACTCATTGTATTTTAACATCTCTTCCAC2160.10540184453227931No Hit
TTTCACGGGATCATTGAGAATTCAGTTTACAAATGATCAGAAATCACAGATA2160.10540184453227931No Hit
GTGCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2150.10491387302981506No Hit
ATCGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2150.10491387302981506No Hit
TAGCATGGGAAGCTTGTGAAGCAGATAATCATATCAGAAATTATCAGTTCAT2140.1044259015273508No Hit
GCTATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG2140.1044259015273508No Hit
CATGCTGGGAGTGGAATGCAGAATGTCAAGCTCATTGCTGCACTGAAGCTAC2140.1044259015273508No Hit
GCTACGGGAGAAAAGTAAGATGGAAGAGGGAAATTTTGCCTGGAAGCCTGGA2140.1044259015273508No Hit
CTATGCGGGAGGACTGATTTCCCCACAGGCACTAGCTTATAAATGCCAAGTC2140.1044259015273508No Hit
ACAGCTGGGTAGAAAACTACTCAGCAATAAAAAGGAACAGACTAATGATACA2130.10393793002488655No Hit
TAACATGGGCACGACCTCTTCCGATCTTGCTACGGGACAAGTCCTAACCATG2130.10393793002488655No Hit
ACCTGCCTCGGCCTCCCAGGGTGCTGGAATTACAGGCGTGAGCCACCGTGCC2130.10393793002488655No Hit
TCAGCTAGGGAAGCAGTGAAATGTTCATTTTGTCCAGCTATTCTGTGTCATT2120.1034499585224223No Hit
TCAGAAGGGACTGATAAATCTGTTGACACAAGTCCTGCAAAGACCTGAGAGG2120.1034499585224223No Hit
ACCAATGGGAAGTGGTTCCTGTGGGTTCCTGATTTGCTTACTCATGAGACCA2110.10296198701995804No Hit
TCTTGTGGGGAAGTTGCTTTTACGATATTATTTTGGTGGCTTTCTTTTCTCT2110.10296198701995804No Hit
GGCAATGGGAGCCGCCGCCGCCGCCGTCGTTGCTACTGCCGCAGCGGAGTTC2110.10296198701995804No Hit
TTGACTGGGATCCAAAATCTTGTATTTTTGGTTCTTCAACATAGTAGCTTTT2100.10247401551749377No Hit
TGACTTGGGCATGTCTTATCCTAGAAAGGAGTTTCCAGATGTCTCAGTTTGT2100.10247401551749377No Hit
GTCTAGGGGGATTCATTCTTAGAGGTTGTCACTTCTATTCAGAATTGCCTTC2100.10247401551749377No Hit
CAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2100.10247401551749377No Hit
ACGTACGGGTCCTGAGCCAGGGGCCTCTGGAAATTCTCTTCCTGATGGTCCT2090.10198604401502952No Hit
TCTTGCGGGGAAATACTTTTTTAATGCAGATAAAAATTATCAGGAAATTAAC2090.10198604401502952No Hit
CAGCTTGGGAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTCTT2080.10149807251256526No Hit
TCACCCGGGATAGAAATGGTACTGATTTTTGTATCCTGCAACTTCAGTGAAT2080.10149807251256526No Hit
TCTTGAGGGATAACTTGGAAATATTTAAGGAAATCATCAGTTGCCTTAAGCA2070.10101010101010101No Hit
TCATACGGGGGTCAAGGCAGCCATCTCGCCGTGAGACAGCAAGTGTCGGATC2070.10101010101010101No Hit
CTGTCTGGGATTGGTGGGAGGGGTGCCAGATGAGAGACTATGGATGAATATA2070.10101010101010101No Hit
ACTGCGGGGAAAGGTATATATTACTCTAGATTTTTCTTACTGTAAATATTGT2070.10101010101010101No Hit
TGCATAGGGAAACACTCAAAATCCAGAATACCTGGATATCCGGCACTTAGAG2070.10101010101010101No Hit
TCTTAAGGGGGAGTTCAGAGAAAAAAATCACTGTTAGAGATATACATGTAGG2060.10052212950763675No Hit
GTCGGTGGGACTGGCAGGTAATTTATATTGTCTTACTTAAGAATTCTCCCCT2060.10052212950763675No Hit
CTCAAAGGGATGGTGCAGTATGCTCCCAATAGGCTCATTCTCCTACAGATAA2060.10052212950763675No Hit
CTACTAGGGATGGCTCCGTAAGGGGCTTGAGGCTTAATGCCCATCCTGTGGC2050.1000341580051725No Hit
ACAAACGGGAAATAATATTTGTTGAGGACATTCCCTTGAAGGCCCTGTGCTA2050.1000341580051725No Hit
GTGCCAGGGGCACTTTTAAAAAAATAATTGGTTGACTTAATATATTTGCTTT2050.1000341580051725No Hit
CACATTGGGCTCTTGTAACAGATTGGTGTGTATTTCTGTGTATCTCTTGTAA2050.1000341580051725No Hit
TCAAAGGGGGAAAACTGAGACGTGCACCCCCATGGGATGGAGACCCGAAGGG2050.1000341580051725No Hit
CCGCTTGGGAAGGATGGGGCTCCAAGGAGATCTCTGAACTTGGAAGACTACA2050.1000341580051725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)