FastQCFastQC Report
Sat 31 Dec 2022
SRR5845892.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845892.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206034
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC7760.37663686576002015No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT6670.323732976110739No Hit
TTAGCTGGGTCCAAAATATAAAAAGATAAAAAGTCATAAAATCATAAATTAA3460.16793344787753478No Hit
CAGTAGGGGAAGTAATCACTTATTTTTTATGTCCATAATTTTATATTGTCTT3270.15871166894784355No Hit
AGTGCTGGGACTGGAAAAAATTAGATGCAATGATATTGACTTAACATGAAAA3080.14948989001815235No Hit
TTGAAAGGGAGTAATTTCAGAATTATCTAGTGGTAATGGTACTTGCAGGATG2850.13832668394536823No Hit
TGTACTGGGGTAATCCTTTTCTGCACACTCGGGTAATTTCTTCTTTTTCATT2780.1349291864449557No Hit
TAATTTGGGCAACGTAAGTGCTAAGATGTGATCATTATTTTTGAAGGAACTG2770.13444382965918245No Hit
GGTTACGGGAGGCCCAAGATAGGGAAGCACTGCTGGTTTACATTTCTGCTCT2660.12910490501567704No Hit
TTGACCAGGGATTTGTTGAGGGCCTACTATGTGCCAGGCACTGGAAATAAAG2630.12764883465835736No Hit
ATCTGAGGGACAAAAACAATAGAGAAAATCAATGGAAGAAGAAAGTGGCTAC2600.12619276430103768No Hit
TCAGAAGGGACTGATAAATCTGTTGACACAAGTCCTGCAAAGACCTGAGAGG2550.12376598037217158No Hit
TGTTCAGGGTATTTTTGATTTATTAGCCTTATTTAAGATTTTGTTTCTTTTC2530.12279526680062515No Hit
ATGCCGGGGCAATTTCAGACACTTTCCAGAGTCTCTGAAGCGCACTCCTAGG2520.1223099100148519No Hit
TTTGTAGGGATCTGTAAAGATCGAGCTTGTGTGTGGTGTCGTGGTCACATCT2500.12133919644330547No Hit
TAGCTAGGGACCTTTTGGAAAACATCAACATTTTAACCATAGACCCTTCAGC2480.12036848287175902No Hit
CAAAAAAAAGGGAAAAAAAAGAGGGAATAGATAATAATATTAAGTACTGGTA2470.11988312608598581No Hit
TAGACTGGGAATTAAGCCATAATCTCAAAGTGGAAAAATATATGTGGTTATT2460.11939776930021259No Hit
GCAACTGGGATTGGCAAGGGATTGATTCTTCAATGGGTTCAGCAATTCACTT2430.11794169894289291No Hit
GTGTGAGGGATGAGCAAAGGTATTTCTAAAATGGCTCTTCTATGAAAAGCAA2420.1174563421571197No Hit
TCGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2420.1174563421571197No Hit
CGAATGGGGGACAGCCTTTTTGTTCAGATTGGCAGTCTCTTTCGCTTATCTG2420.1174563421571197No Hit
CAAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2420.1174563421571197No Hit
GGCATAGGGATTGTAAAAGAAAAGCCTGAAGAAATAAGTCTACAACGATTTA2420.1174563421571197No Hit
GCTACGGGAGAAAAGTAAGATGGAAGAGGGAAATTTTGCCTGGAAGCCTGGA2400.11648562858557325No Hit
TAATTAGGGACAAACAAGATACGTATATTTTTAACTGCTTCAAGTGTTACCT2380.11551491501402682No Hit
ACTAATGGGAAACATTGACCATTTAGAAAATAAATTGATGTAACACTATCTG2380.11551491501402682No Hit
CAGCTTGGGAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTCTT2370.1150295582282536No Hit
GTCATTGGGCAGCAGCTGGAGATGAACCTCAGGAATTTTGCCCAAATCATTG2370.1150295582282536No Hit
ACAACAGGGGCCCTGTGAGCGTCTACAGACAGCTCACCATTTTTGTCCTGTA2360.11454420144248036No Hit
CGCTCGGGGAAGTTGGTGGAGAGCATGCATAATCTCTGCCAGTGTACAGACT2340.11357348787093392No Hit
TCTTGCGGGATGTCTATCAATGAATGAATGGATGAAAAACCATGTGATATAC2330.1130881310851607No Hit
TGTTATGGGATTATTCATTTTATGAAGACAATTTTCTTCTAAAGCTAGATCC2330.1130881310851607No Hit
ACCTGCCTCGGCCTCCCAGGGTGCTGGAATTACAGGCGTGAGCCACCGTGCC2320.11260277429938748No Hit
GCCTCAGGGTAGCAAGATGTGCCATTGGGGAAAACTGGGTAAAAAGAACACA2310.11211741751361426No Hit
ACATTGGGGATAAAATTTAAGTAGTAAATTAATTCCCTGCTATGATTATAAG2300.11163206072784104No Hit
CATCAGGGAGCCCCAGCCCCGACAGGAAATGGGTCGCTCCCCTGTGGACAGT2300.11163206072784104No Hit
TGCATAGGGAAACACTCAAAATCCAGAATACCTGGATATCCGGCACTTAGAG2300.11163206072784104No Hit
GTTGGTGGGCCCAAAATCTGATAATCATGTCTAACCAAGTAATAATGTTTTA2290.11114670394206783No Hit
TAACATGGGCACGACCTCTTCCGATCTTGCTACGGGACAAGTCCTAACCATG2290.11114670394206783No Hit
GAGTGTGGGCCATTTCACAACTATCAGGTACTCTTCTGGGTACTCATCATGA2270.11017599037052137No Hit
ACAACAGGGAGGAAAGATCGAAATTTGACACCCTAACATCACAATTATAAGA2270.11017599037052137No Hit
GAGAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCC2260.10969063358474815No Hit
AGAATGGGGAAGTGGAAAATGACTTGATACAGGTCTGTAGGCCTCTATGGAG2260.10969063358474815No Hit
TATGACGGGCTGGAGACCACATCCTGTCCATCGATGGCACCAGCATGGAACA2260.10969063358474815No Hit
CGACACGGGCCTGCCCGCTTCCTTGGGCTCCCCGGCCCTGGGTGGGCGGTGC2260.10969063358474815No Hit
ACAGCAGGGCCACTCTATGCTATTAAAAGGGCTAGAATTTGGAAGATGATGG2250.10920527679897493No Hit
ATTCGCTGGGGGTGGGCCGGGCCGTGTGGCTGCGCTTCTCCCCGCCGCTGCC2250.10920527679897493No Hit
GTCTAGGGGGATTCATTCTTAGAGGTTGTCACTTCTATTCAGAATTGCCTTC2250.10920527679897493No Hit
AGTTTTGGGACTGAAGGCAAATCTCCTTTGTCCATTTACCTGGAAACCAGAA2240.10871992001320172No Hit
TCTGGTGGGAAGTGTATTTGTACACTGATAAACTGATCTTCCGAAGAGACTG2230.10823456322742847No Hit
GTTTTTGGGAAGACTAGGCAAGCCACAATTTGAGAAAAATCAGTCAGCTGGC2230.10823456322742847No Hit
CTTGCTGGGCAACTCCAGCTGGATTATTTTGGAGCCTGCAAATCTATTCCTA2230.10823456322742847No Hit
AGTTAGGGGCTTCTGCTCTGAACCACTGTCGGTGCATCCATATTAATAAAAG2220.10774920644165525No Hit
AGCTTAGGGATTGTTTTATAAGCCTACTTTACCTCCCAGTCTTGGAAACAAG2220.10774920644165525No Hit
GCTATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG2220.10774920644165525No Hit
GGCAGCAGACTTCTCAGTGGAAACCTTATAGGCCAGGAGAGAGTGGCATGAC2220.10774920644165525No Hit
TCTACAGGGAGACGGAGGTCACTGGTGACCACATTCCCACTCCGCAAGACCT2220.10774920644165525No Hit
TCAGCTAGGGAAGCAGTGAAATGTTCATTTTGTCCAGCTATTCTGTGTCATT2210.10726384965588204No Hit
GTTTACGGGGGAGCGGAGAGGGTTATCATTTAACATGGAAGAAGATGAGTTC2200.10677849287010882No Hit
AGCACAGGCTGCCAGCTTGATGTTTTCAAATTCAGCTTTGTAGGAGGCCATA2200.10677849287010882No Hit
CACGTAGGGCTACAAAATTAACCAAAAACTCTGACATATTAATGTGGTATTA2200.10677849287010882No Hit
AAATATGGGGCACCTGGGAACTCATCTGCTGCCTCTTGGTTGGCAGAAGCTG2200.10677849287010882No Hit
GTGCCAGGGGCACTTTTAAAAAAATAATTGGTTGACTTAATATATTTGCTTT2190.10629313608433559No Hit
CAGTGCGGGCGGCTGGGGGTCGCCGGCCAGTGGCGCTTCGTGGACGTGCTGG2190.10629313608433559No Hit
CTTTCTGGGCAAAAAATGATTCTTGTATCAAAGTAAAAGATACAAAAGACCG2190.10629313608433559No Hit
GACATAAGGGGATCTATTGTATTTGAAACATAACTTTGACAATTATTAGTGT2190.10629313608433559No Hit
TCAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2170.10532242251278916No Hit
CTTTTACGGGGTGATTTAATCTGTTAATATGAATATCTCATTTAAAACTTTT2170.10532242251278916No Hit
CTATGCGGGAGGACTGATTTCCCCACAGGCACTAGCTTATAAATGCCAAGTC2170.10532242251278916No Hit
CATGTTGGGGAGGAACCCGAGGCACAGAGAAGTAAAATAATTTTCACAAGGT2170.10532242251278916No Hit
GGCAATGGGTTCAAAGAAAAGTCAGTGGAAGTATGAATTTGGGATTTTATAT2170.10532242251278916No Hit
AATGTTGGGGTTTTGGATAAATTTTTATAGTGGAGAAAGGTTACTTTGATCT2170.10532242251278916No Hit
AGCCTCGGGGTTTCGGGGGTTGCAAGATGGCGGCCACCAGTGGAACTGATGA2170.10532242251278916No Hit
TTGACTGGGATCCAAAATCTTGTATTTTTGGTTCTTCAACATAGTAGCTTTT2160.10483706572701594No Hit
TTAAGCCGGGAATATAGCTATTTTAAAAATAATAAACACATTCATATAATAA2160.10483706572701594No Hit
TTATAAGGGATTTTCTTCGTGGGGTAAACAATGGCTAATGATCAGGCTTTCC2160.10483706572701594No Hit
TAACCCGGGCAGCCTCCCGAGTAGCTGGGATTACAGATGTGAGCCACAGCAC2160.10483706572701594No Hit
AGCTACGGGTGCAGGGAGTGGGATCGTGAGGGATCGGGAAGCAGTGTCAGTG2150.1043517089412427No Hit
AGTACAGGGGCATTTCACGAAGTTTGATGTCTTGTTCTTCCATTGTTTCATC2150.1043517089412427No Hit
GCAACTGGGAATGAAAGAAAACTACAAAAATAATACTTCTTTTCCACCCGTC2140.10386635215546948No Hit
ATGAGAGGGATGAGGTGGCCCCTGAGAGGTGGGGGCCTCTCCAGGGCACATC2140.10386635215546948No Hit
TCTTGCGGGGAAATACTTTTTTAATGCAGATAAAAATTATCAGGAAATTAAC2140.10386635215546948No Hit
GGCATGGGGACCACTTTCTTTTCCTTTTTAGAGGCCACAGCAGTTTTGATGT2130.10338099536969626No Hit
TTTCCGGGATGTGGGAGAACTATATTAGGTGTGCCTTTATTTGGAACAGAGT2130.10338099536969626No Hit
CGTAGCGGGATGCTCCAAAATGTTCACTAAAATGGGATAAACATAAGGCATT2130.10338099536969626No Hit
TCGAATGGGACATTTCCAAATTGATCACTTGTAACAAAATAGCTAACATAAT2130.10338099536969626No Hit
CGTAGAGGGGGAACATAATGAAGCTGGTTGGCTGCTCCTAAGTTCAGTGGAA2130.10338099536969626No Hit
AACCAGGGGGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCC2120.10289563858392305No Hit
TGTGGGGGGTAAAGAGCCAGAGTGGGAACTGTTGATCCAAGTGTAGCCTGAA2120.10289563858392305No Hit
TGCCTGGTAAAGAGGTAGAACTGGTTAGATACAGGGGTTTGTTTTGTTCAGT2120.10289563858392305No Hit
TACCAAGGGGTGTGAGATGAGGGTCTCAATTTTTATCTCTGCATGTGGATAT2110.10241028179814982No Hit
GGTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2100.1019249250123766No Hit
TAAACGGGGGGGAGGAGTGATGTTCATTTTGGGATTTGCAGGGTGCATTGGA2100.1019249250123766No Hit
GGTTCCGGTGTGTGTGTGTGGACAAGAAGTCAGAGGCAATAGATTGAGGAGT2100.1019249250123766No Hit
ACAGCAGGGATTAACGAGGCTGTAGAAGTACTGAACAACACCATCAGCCAAC2100.1019249250123766No Hit
TAGATCTGGGATGCCAGCTCCACCATTGAGGATGATCATGAAGGAAGCTTTA2100.1019249250123766No Hit
GTCTGCGGGCATGAAATTTCTCTGTGACATTTGCAGCTTTTGCTCTAAGGAT2090.10143956822660338No Hit
ATTCATGGGATGCCAGTAAATCTTCTCAAAAAAGAATAATGAGGCCCATTTG2090.10143956822660338No Hit
AGCTCAGGGGCCGCCCGCGCCGCGCCGGAGTCCGCTGTCCGCCAGCTACCCG2090.10143956822660338No Hit
TAAATGGGGTCACTAAATACTGGAATATAAAAAATTTGACAAAATATTTTCA2090.10143956822660338No Hit
AATAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2080.10095421144083017No Hit
TCATACGGGGGTCAAGGCAGCCATCTCGCCGTGAGACAGCAAGTGTCGGATC2080.10095421144083017No Hit
TCTTAAGGGGGAGTTCAGAGAAAAAAATCACTGTTAGAGATATACATGTAGG2080.10095421144083017No Hit
GTGATGGGGGTTTTTAGCAAAATCACTAGGTTTTAGGATCCTTCTTTATTTA2080.10095421144083017No Hit
ATCGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2070.10046885465505692No Hit
TTGCTTGGGGGTTAATCATTAAAATGACAGAAAAATTATAATTCTGTATGCA2070.10046885465505692No Hit
CACATTGGGCTCTTGTAACAGATTGGTGTGTATTTCTGTGTATCTCTTGTAA2070.10046885465505692No Hit
CAATTAGGGGAGATAATGGTTGTAGATATATGTGCATTCAACATGAGAGTCC2070.10046885465505692No Hit
TCATGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2070.10046885465505692No Hit
TTTGCAGGGGGAATTGGAAAATTGGAGTAGCCATTTACTGAGATAGAATAGA2070.10046885465505692No Hit
TTCCATGGGACCACAAAGTTACTTCTGGATAAGAGCAACCCCAAGATACTAA2070.10046885465505692No Hit

[OK]Adapter Content

Adapter graph