FastQCFastQC Report
Sat 31 Dec 2022
SRR5845870.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845870.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174162
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT9830.564417037011518No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC9220.5293921751013424No Hit
TTAGCGCGGGATTGCTTTTACAAAGGTGCAAAAACTTTGAGGAGTTAGAAAA2910.16708581665346056No Hit
ACAAATGGGCTGCTACCACAAAAACACTTGTACATAGTCCATATATGCTACA2700.15502807730733456No Hit
ACGTTTGGGAGATAATCATAAATAAGAATCAAATAGCAATTCTGGAACTGAT2370.13608020119199365No Hit
TCAGACGGGCAAAAATTTCAGTGGTAACAGGTAAAATGATTCTACTACACAC2250.1291900644227788No Hit
GACATGGGATTGGAAAAAGGGAAACAGTTGCAGAATTCTCCAAGGAACCAAT2220.12746753023047508No Hit
AAGGTTGGGGAAGCCATTTTGTCTGTTTAAAATTTCAGTTTAATTTTGCTTT2220.12746753023047508No Hit
TTAAATGGGTACTGGAGGCAATTTCTAACACAATGACAAGTATTTGTGTCTC2190.12574499603817135No Hit
GTTTGAGGGCTCTGCTCAGATTACATGGGTAAGTTTGCTCCTTACAACTAGA2160.12402246184586765No Hit
ATGTACGGGATAATACAACTTAGTACATTAAAGCTACGAAAACTCATCCTGG2140.12287410571766516No Hit
GACCGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2140.12287410571766516No Hit
ACAAGTGGGATCATTTTAGTGTGTTACAATAGTAACAGTATGCAAAAGCAGC2120.12172574958946268No Hit
GTTACTGGGCGATCTACTCACGGGCAGTTACAACCTAGAGAGATTTTGAGCC2120.12172574958946268No Hit
ATTCCAGGGAGAAAATAGGGGTTAAGACTGATAACAACCTTTTACATTGTGA2090.12000321539715895No Hit
AGCACTGGGATGTGAGGTAAATTGATTGGAATATCCTCAGATTGCTTTACAT2060.11828068120485526No Hit
TAACTCGGGAATCTTAAATTTAGCTTGTCTTTCTGCTAAGCTGTTAAATGTA2060.11828068120485526No Hit
GTTTCAGGGGTTAATAGTAACAATACGTTCCATGAATTATGCAGTTGGGATG2060.11828068120485526No Hit
CTACTTGGGGTCATTTTACACCCCCACCAGCAATGTGTGTGGGTTCTAATTT2050.11770650314075401No Hit
CGCATTGGGGGCATGTACCTGTCTCTATATCAACATCTTTCAATTCAGGACC2040.11713232507665278No Hit
AGCCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2010.11540979088434905No Hit
GAGACGGGATAGTCACTGAAGACAGAAGTTTGGTATTTGGTATAGTCTACAA1990.11426143475614658No Hit
TCTTCGGGGACTTCGCTGACTTTAAAAATATTAATTTTAAACCACTGGACAT1990.11426143475614658No Hit
CTACTGGGTCCTAGTCTCTGATAAAACAGACTTTAAACCAACAAATATCAAA1980.11368725669204534No Hit
CACCTAGGGGTAAGAAGTAATTCAAATATCTGCTTGTGGGTCAATAAAAAGG1970.1131130786279441No Hit
AAGCTGGGGACTGTGATCATGATGAAAATGAATGAGGATGGCCTGACGCCTG1970.1131130786279441No Hit
GGTATAGGGACCACATTATAAATGAATGCTTGGATGTAAGGGCAGACCTGCA1960.11253890056384286No Hit
TGACTCGGGGACACATTGTGTTCACGGAATGGAAAACTCACTGTTGTTGAGA1960.11253890056384286No Hit
GCGCTTGGGATTGACTTTCTTCACAGAATTGGAAAAAAAACTACTTCAAATT1950.11196472249974163No Hit
GGCCTAACTGGATGTGTTAAAATCCAGATACCCAGACCTTCTAAGAGGTTCC1950.11196472249974163No Hit
CACACAGGGAAGATGATCGTTCCAGACAGAGAAATTCTCAGGACATGAAAAC1950.11196472249974163No Hit
GGGAAAGGGATACATTTTGAGTTGTTCATAAAGTTTGTCTTGTGATAGTCCT1950.11196472249974163No Hit
TAGCATGGGTGGTGGGGTAAAGCTTGAAGATTTGATTGTAAAAGACGGGCTA1950.11196472249974163No Hit
GTTAGTGGGGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTGC1930.11081636637153913No Hit
TCTTCGGGGGAAAATTTATCCTTTCAACTTTCTTTAGGTGCCAGCATTATTT1930.11081636637153913No Hit
GTTAAAGGGCGATCTAGCTTAGGGGTAAATATTATGGTTACTTTTTATCTCA1920.1102421883074379No Hit
CTTGGCGGGATAATATAATTGACAAAATATGCTAAAAGGCAAAAAGTGGTGT1920.1102421883074379No Hit
ACTAATGGGATTCCAAAGTGTCAGTTTTATGTGATAGTTAAAACAGTTTGTG1920.1102421883074379No Hit
CTCATTATGGGAGTAGAGCCTCAAAATAATAAGCAAAAAATAACAGAACTAA1900.10909383217923543No Hit
TGTAGAGGGGAGAAAAATGGGGCATTTCAAAAAGGCTTAGACACTAATGGGA1900.10909383217923543No Hit
GTAACGGGAGTCCACATGAGGAATGTGTTGGAGAGTTGAATTACATTCAACA1890.10851965411513419No Hit
AAATGTGGGTCTGAATAAAAAATAGAACTATAAAAAGTAATATAGCATTGCT1890.10851965411513419No Hit
AACGCTGGGCTGTGATTTGACCCTTGAGGCACCCTTGCTGTTTCAGTCATGG1890.10851965411513419No Hit
CTGTGTAGGCCTACGCTAGTATGTGCATTTGTGTCTTTGTTTTTAACAACAA1880.10794547605103295No Hit
GACTCAGGGAATGTATTGTTATTAACTGTAGTCAACGTGATGTACAATGGGT1880.10794547605103295No Hit
ACAACTGGGGGGAAAACTGGAAGAAGCACACCCCACTCTGATTATTCCACCA1870.10737129798693171No Hit
TCACAGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGG1870.10737129798693171No Hit
GTTGCAGGGGTAGTAATAACTTCCCAGCACCTGGACATCTCTTCCAGAGTTA1870.10737129798693171No Hit
GTTGCTGGGATACCTCTTCTGATAGTTTTATTAAGCATGGAGAAATTGCCAA1870.10737129798693171No Hit
TCAACAGGGAACATCTTTGTTACTAAATCTTTGTTCATATCCACAATTACTT1870.10737129798693171No Hit
AATAGAGGGTCAAATTTTTATGGGGCAACTTTTTGGTATGCATTAATGTTTA1870.10737129798693171No Hit
GGCTATGGGATCTAAGAAATTTTGCCTAACCCAAAGTCACAAAGATTTCCTT1860.10679711992283046No Hit
CAGTCAGGGAACATTAATGATTTTTGTTTTTCCAGACTATTACAGAAAAACA1860.10679711992283046No Hit
ACATTTGGGATTTGCTTTGTTGGCATCTGTGTTTATTTACTTGTACACCACA1860.10679711992283046No Hit
TGTACAGGGGTCTTTAAGTGAACTATTGCCATGTGACATTTGAGAACGACAT1860.10679711992283046No Hit
TACTGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1850.10622294185872923No Hit
TGTTCTGGGCTTCCGATCCGTATTTGGGTCTGTCTCTGTGGATTTACCTATT1850.10622294185872923No Hit
ATCAGTGGGATTTTCAAACATTGTTCCTCGGACTGCTGAGAACTTCAGAGCA1850.10622294185872923No Hit
AACTGCGGGAGATCATGCTATCCCTAAGCAAAACAGACAGACAAACAAACAA1850.10622294185872923No Hit
TGGCCTCCCAAAGTGCTGGGATTATAGGAGTGAGCCACCATGCCCAGCCTGG1850.10622294185872923No Hit
TTATGCGGGGATGATCTCTTTTCACCATTTTTCTTGCCAGCTTCTTGCTCAT1840.10564876379462798No Hit
CAGGCTGGGTAAAGTTGGTGAATGATCTGGCCCACCAACTTGCCATTTCTGA1840.10564876379462798No Hit
AACTATGGGTCTAGGAAAGGGAGGCTAGAAGAATCAAGTTGTCAGCATTGTT1840.10564876379462798No Hit
TATCGCGGGCACCCCAGTCTTGGCAAGGTTCATGCCCCCATCTCAACTATGT1840.10564876379462798No Hit
TATACAGGGAGACTTCCCTTACTGCAAGCTGTTTTACCAGCTGAAGAATAAT1830.10507458573052675No Hit
ACAATGGGGCACCCAGCAGATTAATTTGTTATTAGCACCTTAGGATAAATTC1830.10507458573052675No Hit
CGCTGGGGAGCCAAATCATGAGTGAACTCTCATTCACAGTTGCTACAAAGAG1820.10450040766642552No Hit
ATTACAGGGAACCTTTCTAAAGGCAATACTTAGAAAAATTCTATGGCAGCAC1820.10450040766642552No Hit
CAAGTCGGGGGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGA1820.10450040766642552No Hit
AGCAGTGGGGAGCCCAGGAATTTGAGGCTCCAGTGAGCTATGATTGTGCCAC1810.10392622960232428No Hit
AGTGCTGGGATTGCAGGCGTGAGCCACCGCGCCTGGCCTGACCCCAGAGCTT1810.10392622960232428No Hit
GATTAGGGGGATCTTTGGCTTAACAATGAAATCAAGGTGGAAATTAAAAAAT1810.10392622960232428No Hit
ACTATAGGGAGGTGTGAGCCCCTATGCCTGGCCTTACTGTAGTTACATCTAA1810.10392622960232428No Hit
GCTCACGGCAACACTTCTTTATGATAAAAACTCTCAGAAAAATAGTAATAGA1810.10392622960232428No Hit
TCTGTCGGGTCCTTGCTTACAGTAGAATGCCAAAGGGCAACTGATGGAGGGA1810.10392622960232428No Hit
AGACTGGGGAGGGTCCACTCTCAGGGTTTATTTGTGAGTTGTGTTTGTTGTG1800.10335205153822304No Hit
CGACTAGGGGCCGAGTATTTCAAGGATAGCAGAAATGTGAAGAAGCTTAAAG1790.1027778734741218No Hit
AGATACGGGTGCGAGGGCATTATGAAGTTGTCACTCTCATAGCAGATGGCGG1790.1027778734741218No Hit
TATTACGGGGATTTCAAAATTTTCAGCTAGGAATGCAAAACTGAGAGAGAAA1790.1027778734741218No Hit
CAGAATGTGGCTTACTTCAGTAAATATTTCATGGGCACTTGAAAAGTATTTG1780.10220369541002056No Hit
CCGAATAGGCAGAGACTGTTCTAATTTTTATTACCTTGGAGTAATTTTCCTT1780.10220369541002056No Hit
TGCCTTGGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGT1780.10220369541002056No Hit
ACTACAGGGATACAAAATGCTCTGATAATCATCATTGCATTATATTTTTATT1770.10162951734591931No Hit
TCACATGGGAAGTCATCTAACACAAATACATTGAGAGAAGTTTTCCTAGCAT1770.10162951734591931No Hit
AACGTAGGGATAACCTGTAAGAGTATTCATTAAAACGTTCAACTTTTTCAAG1760.10105533928181808No Hit
CTAGTTGGGAGGCTGTAATGTTTGATGAAGATCTAAGAATAACTGAAAGTAA1760.10105533928181808No Hit
AACATTGGGAACATGCCCATATTTTACTTGGAAATATTAAAAGAAAGGGTTC1760.10105533928181808No Hit
TATAAAGGGGCATGGCACAGTTTTGAACCTAATTCTGTGTTTTGCATTTTTA1760.10105533928181808No Hit
GCAGGTGGGGTATTTGACAATAATCTTATATTCACCTTAAATTCTCTTATTT1760.10105533928181808No Hit
GTCATTGGGATCTTTAAAAATTCACTTGGATACTGAAGTTTAGCTTGACAGA1760.10105533928181808No Hit
TTGAGGCGGGTACAGGTGGGCCTTTCAGAGACAACAAGACAGGAGAATTATT1750.10048116121771683No Hit
TCTTTGGGCTCATGGGCTGTGGGGTCTGACAGCCTGACTCTTTGGCTGATTA1750.10048116121771683No Hit
AGTTTTGGGAATCTTGGGGTTTAACTCCTTCCTCTCACACCTCACACCCTAA1750.10048116121771683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)