FastQCFastQC Report
Sat 31 Dec 2022
SRR5845869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169824
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT9890.5823676276615791No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC9080.5346711889956661No Hit
TTAGCGCGGGATTGCTTTTACAAAGGTGCAAAAACTTTGAGGAGTTAGAAAA2900.17076502732240437No Hit
ACGTTTGGGAGATAATCATAAATAAGAATCAAATAGCAATTCTGGAACTGAT2840.167231957791596No Hit
GACATGGGATTGGAAAAAGGGAAACAGTTGCAGAATTCTCCAAGGAACCAAT2320.1366120218579235No Hit
ACAAATGGGCTGCTACCACAAAAACACTTGTACATAGTCCATATATGCTACA2290.1348454870925193No Hit
GTTTGAGGGCTCTGCTCAGATTACATGGGTAAGTTTGCTCCTTACAACTAGA2250.13249010740531375No Hit
CTGTGTAGGCCTACGCTAGTATGTGCATTTGTGTCTTTGTTTTTAACAACAA2210.13013472771810816No Hit
CTTGGCGGGATAATATAATTGACAAAATATGCTAAAAGGCAAAAAGTGGTGT2150.1266016581872998No Hit
GTTACTGGGCGATCTACTCACGGGCAGTTACAACCTAGAGAGATTTTGAGCC2140.1260128132654984No Hit
AAGCTGGGGACTGTGATCATGATGAAAATGAATGAGGATGGCCTGACGCCTG2120.12483512342189561No Hit
AAGGTTGGGGAAGCCATTTTGTCTGTTTAAAATTTCAGTTTAATTTTGCTTT2120.12483512342189561No Hit
GGCTATGGGATCTAAGAAATTTTGCCTAACCCAAAGTCACAAAGATTTCCTT2060.12130205389108725No Hit
GACCGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2060.12130205389108725No Hit
ATTCCAGGGAGAAAATAGGGGTTAAGACTGATAACAACCTTTTACATTGTGA2050.12071320896928585No Hit
TCAACAGGGAACATCTTTGTTACTAAATCTTTGTTCATATCCACAATTACTT2040.12012436404748444No Hit
ACTAATGGGATTCCAAAGTGTCAGTTTTATGTGATAGTTAAAACAGTTTGTG2030.11953551912568307No Hit
TTAACTGGGGTCTTCATAATTTTTCAGTAGCTTCTTTCAATAGGAAACATTT2020.11894667420388166No Hit
TTATGCGGGGATGATCTCTTTTCACCATTTTTCTTGCCAGCTTCTTGCTCAT2020.11894667420388166No Hit
AATTGTGGGGTATTGAGAATGAACACCTTTACTTTGCCTCATTCCTAGTACC2020.11894667420388166No Hit
GGGAAAGGGATACATTTTGAGTTGTTCATAAAGTTTGTCTTGTGATAGTCCT2010.11835782928208027No Hit
GTTAGTGGGGACTTAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTGC2010.11835782928208027No Hit
CTACTTGGGGTCATTTTACACCCCCACCAGCAATGTGTGTGGGTTCTAATTT2000.11776898436027887No Hit
GGTATAGGGACCACATTATAAATGAATGCTTGGATGTAAGGGCAGACCTGCA1990.11718013943847749No Hit
CGCATTGGGGGCATGTACCTGTCTCTATATCAACATCTTTCAATTCAGGACC1990.11718013943847749No Hit
CTATGCCGGGATAAGCAGTTGCTTCAAAATGTGAATTAACTTGTTGAAACTG1990.11718013943847749No Hit
AGCACTGGGATGTGAGGTAAATTGATTGGAATATCCTCAGATTGCTTTACAT1980.11659129451667609No Hit
CCCAGGGGGAGAAATTAACAAACAAAAGACAGCCCCACACGGACCAGTGTGC1980.11659129451667609No Hit
TTAAATGGGTACTGGAGGCAATTTCTAACACAATGACAAGTATTTGTGTCTC1970.11600244959487468No Hit
CAGAATGTGGCTTACTTCAGTAAATATTTCATGGGCACTTGAAAAGTATTTG1960.11541360467307331No Hit
AATGAGGGACAGTGTAAATGTGTTCCTATTTCTCTGGGGCCTTGCCAGCATC1960.11541360467307331No Hit
GATTAGGGGGATCTTTGGCTTAACAATGAAATCAAGGTGGAAATTAAAAAAT1960.11541360467307331No Hit
CAGGCTGGGTAAAGTTGGTGAATGATCTGGCCCACCAACTTGCCATTTCTGA1950.1148247597512719No Hit
CCACACGGGATTCATGACTAAGGAGGATTAATAATTAACATGAGCTGTAGAA1930.11364706990766912No Hit
AGATACGGGTGCGAGGGCATTATGAAGTTGTCACTCTCATAGCAGATGGCGG1930.11364706990766912No Hit
GAGGTTGGGGTATTTTATGAATAAAAAATAAAATAATTTGTATTCATTGAGC1910.11246938006406633No Hit
TCTTCGGGGACTTCGCTGACTTTAAAAATATTAATTTTAAACCACTGGACAT1900.11188053514226493No Hit
ATGTACGGGATAATACAACTTAGTACATTAAAGCTACGAAAACTCATCCTGG1880.11070284529866214No Hit
AACTATGGGTCTAGGAAAGGGAGGCTAGAAGAATCAAGTTGTCAGCATTGTT1870.11011400037686074No Hit
TCACAGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCGG1870.11011400037686074No Hit
TTGTGAGGGATTCCACTTAAACCACATCAACAGAGAATGGGTAAGATGTGTT1870.11011400037686074No Hit
AGACTGGGGAGGGTCCACTCTCAGGGTTTATTTGTGAGTTGTGTTTGTTGTG1860.10952515545505936No Hit
TGGCCTCCCAAAGTGCTGGGATTATAGGAGTGAGCCACCATGCCCAGCCTGG1860.10952515545505936No Hit
TGTACTGGGATTTTCATTAAAGGATGTAGTTCTTTCATCCCATTCATGTAGA1850.10893631053325796No Hit
GTTTCAGGGGTTAATAGTAACAATACGTTCCATGAATTATGCAGTTGGGATG1850.10893631053325796No Hit
ACATTTGGGATTTGCTTTGTTGGCATCTGTGTTTATTTACTTGTACACCACA1850.10893631053325796No Hit
GTTGCTGGGATACCTCTTCTGATAGTTTTATTAAGCATGGAGAAATTGCCAA1850.10893631053325796No Hit
CTACTGGGTCCTAGTCTCTGATAAAACAGACTTTAAACCAACAAATATCAAA1850.10893631053325796No Hit
GAGACGGGATAGTCACTGAAGACAGAAGTTTGGTATTTGGTATAGTCTACAA1840.10834746561145657No Hit
TTGTCCGGGAAGACTGTAGGTTTATGGGAGATATATAGTGGATTAGTTCTTC1840.10834746561145657No Hit
ACAATGGGGCACCCAGCAGATTAATTTGTTATTAGCACCTTAGGATAAATTC1840.10834746561145657No Hit
TGTAGAGGGGAGAAAAATGGGGCATTTCAAAAAGGCTTAGACACTAATGGGA1840.10834746561145657No Hit
TACAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1830.10775862068965517No Hit
GTTAAAGGGCGATCTAGCTTAGGGGTAAATATTATGGTTACTTTTTATCTCA1820.10716977576785376No Hit
CGACTAGGGGCCGAGTATTTCAAGGATAGCAGAAATGTGAAGAAGCTTAAAG1820.10716977576785376No Hit
GCTCACGGCAACACTTCTTTATGATAAAAACTCTCAGAAAAATAGTAATAGA1810.10658093084605239No Hit
TCTGTCGGGTCCTTGCTTACAGTAGAATGCCAAAGGGCAACTGATGGAGGGA1800.10599208592425098No Hit
GCGCTTGGGATTGACTTTCTTCACAGAATTGGAAAAAAAACTACTTCAAATT1790.1054032410024496No Hit
TTTGACCGGGGGGCCTGGTCAGTGCGTCCATTTCTAGTCTCTTCTCAAGGAC1790.1054032410024496No Hit
TGACTCGGGGACACATTGTGTTCACGGAATGGAAAACTCACTGTTGTTGAGA1790.1054032410024496No Hit
TTAAATGGGCTCTCCCTCCTGCCTAGCTAGGAAAGAGAAGAAAAAACTAAGC1780.1048143960806482No Hit
AACGCTGGGCTGTGATTTGACCCTTGAGGCACCCTTGCTGTTTCAGTCATGG1780.1048143960806482No Hit
AATAGAGGGTCAAATTTTTATGGGGCAACTTTTTGGTATGCATTAATGTTTA1780.1048143960806482No Hit
TCCTTGGGGACATGCAAGAAGTAAGAGCATTTGTGGCTTGAACTTGCCAGAT1770.10422555115884681No Hit
ATCAGTGGGATTTTCAAACATTGTTCCTCGGACTGCTGAGAACTTCAGAGCA1770.10422555115884681No Hit
CAAAATGGGCTCCCAGTTCCCCCAAACATGAGCTCAGGATGTAGTTAATTAA1760.1036367062370454No Hit
ACAACAGGGAAGTTCTTTTAATGAATATCAGTTTTAATTTATAGACTGCCAT1750.10304786131524402No Hit
CACCTAGGGGTAAGAAGTAATTCAAATATCTGCTTGTGGGTCAATAAAAAGG1750.10304786131524402No Hit
TATTACGGGGATTTCAAAATTTTCAGCTAGGAATGCAAAACTGAGAGAGAAA1750.10304786131524402No Hit
GTTGCAGGGGTAGTAATAACTTCCCAGCACCTGGACATCTCTTCCAGAGTTA1750.10304786131524402No Hit
TCAGACGGGCAAAAATTTCAGTGGTAACAGGTAAAATGATTCTACTACACAC1740.10245901639344263No Hit
CACAGCGGGATGGGCTAAAGTAACAAAGACTAGAAAAACAGCCAATTTTCTA1740.10245901639344263No Hit
AACGTAGGGATAACCTGTAAGAGTATTCATTAAAACGTTCAACTTTTTCAAG1740.10245901639344263No Hit
ACTCATGGGATCATCTCCCCATCTCCAGATCCTTAATATCATCACATCTGTG1740.10245901639344263No Hit
TTGATAGGGCTGTTGTGACTTGGAACCAGTGAGAAGATGGCATGGGGAGGGG1730.10187017147164122No Hit
TAGCATGGGTGGTGGGGTAAAGCTTGAAGATTTGATTGTAAAAGACGGGCTA1720.10128132654983985No Hit
CGTAAAGGGTGGGAGAAGGTGCTTAACATGGCTGATTTTTTTTCTTCTTTTC1720.10128132654983985No Hit
TGTCCCGGGAATCACAGGTGACTTGGCATGAAATTGCAAGGCCTCAGATACA1720.10128132654983985No Hit
CCCAATGGGAACTCATTGCCCTTCATGGAATCGCATGCCCGTTTCCCCCCAC1710.10069248162803844No Hit
TTCATGGGGCTTATTTCTAGCCATGAGTTTTGTGAGGAACCAGAGGCTACTT1710.10069248162803844No Hit
ACTATAGGGAGGTGTGAGCCCCTATGCCTGGCCTTACTGTAGTTACATCTAA1710.10069248162803844No Hit
TGCCTTGGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGT1710.10069248162803844No Hit
TGTGCTTGGGTACTAATGTGTACAGATCTTCTTTCTAAGGTGGTGAAAATAT1700.10010363670623704No Hit
CAGCGAGGGATTGTGCTTCCAGCTTCTAGCATGGTGTCTGGTGTTATTGTGA1700.10010363670623704No Hit
CTCATTATGGGAGTAGAGCCTCAAAATAATAAGCAAAAAATAACAGAACTAA1700.10010363670623704No Hit
GTAACGGGAGTCCACATGAGGAATGTGTTGGAGAGTTGAATTACATTCAACA1700.10010363670623704No Hit
TTGTTAGGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGT1700.10010363670623704No Hit
AAATGTGGGTCTGAATAAAAAATAGAACTATAAAAAGTAATATAGCATTGCT1700.10010363670623704No Hit
TAACTCGGGAATCTTAAATTTAGCTTGTCTTTCTGCTAAGCTGTTAAATGTA1700.10010363670623704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)