FastQCFastQC Report
Sat 31 Dec 2022
SRR5845868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170638
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT9550.5596643186160175No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC8860.5192278390510906No Hit
TTAGCGCGGGATTGCTTTTACAAAGGTGCAAAAACTTTGAGGAGTTAGAAAA2800.16409006200260198No Hit
ACGTTTGGGAGATAATCATAAATAAGAATCAAATAGCAATTCTGGAACTGAT2620.1535414151595776No Hit
TCTTCGGGGACTTCGCTGACTTTAAAAATATTAATTTTAAACCACTGGACAT2440.14299276831655316No Hit
CTACTTGGGGTCATTTTACACCCCCACCAGCAATGTGTGTGGGTTCTAATTT2270.13303015740925234No Hit
ACAAATGGGCTGCTACCACAAAAACACTTGTACATAGTCCATATATGCTACA2270.13303015740925234No Hit
GACATGGGATTGGAAAAAGGGAAACAGTTGCAGAATTCTCCAAGGAACCAAT2260.13244412147352874No Hit
AAGGTTGGGGAAGCCATTTTGTCTGTTTAAAATTTCAGTTTAATTTTGCTTT2240.1312720496020816No Hit
GGCCTAACTGGATGTGTTAAAATCCAGATACCCAGACCTTCTAAGAGGTTCC2210.12951394179491085No Hit
CTATGCCGGGATAAGCAGTTGCTTCAAAATGTGAATTAACTTGTTGAAACTG2210.12951394179491085No Hit
GTTTGAGGGCTCTGCTCAGATTACATGGGTAAGTTTGCTCCTTACAACTAGA2140.12541169024484583No Hit
GATTAGGGGGATCTTTGGCTTAACAATGAAATCAAGGTGGAAATTAAAAAAT2110.12365358243767509No Hit
GTTGCTGGGATACCTCTTCTGATAGTTTTATTAAGCATGGAGAAATTGCCAA2110.12365358243767509No Hit
CTCATTATGGGAGTAGAGCCTCAAAATAATAAGCAAAAAATAACAGAACTAA2080.12189547463050433No Hit
TTATGCGGGGATGATCTCTTTTCACCATTTTTCTTGCCAGCTTCTTGCTCAT2080.12189547463050433No Hit
CTTGGCGGGATAATATAATTGACAAAATATGCTAAAAGGCAAAAAGTGGTGT2080.12189547463050433No Hit
CTGTGTAGGCCTACGCTAGTATGTGCATTTGTGTCTTTGTTTTTAACAACAA2070.12130943869478077No Hit
AATAGAGGGTCAAATTTTTATGGGGCAACTTTTTGGTATGCATTAATGTTTA2060.12072340275905719No Hit
TGTACTGGGATTTTCATTAAAGGATGTAGTTCTTTCATCCCATTCATGTAGA2050.1201373668233336No Hit
GTTACTGGGCGATCTACTCACGGGCAGTTACAACCTAGAGAGATTTTGAGCC2020.11837925901616286No Hit
GGCTATGGGATCTAAGAAATTTTGCCTAACCCAAAGTCACAAAGATTTCCTT2010.1177932230804393No Hit
GGGAAAGGGATACATTTTGAGTTGTTCATAAAGTTTGTCTTGTGATAGTCCT2010.1177932230804393No Hit
AACGCTGGGCTGTGATTTGACCCTTGAGGCACCCTTGCTGTTTCAGTCATGG1990.11662115120899214No Hit
GTCGGCGGGATAGAGCTGGGATTTTTACAAACCGTGAATTAGAATTTATTCT1970.11544907933754497No Hit
AAGCTGGGGACTGTGATCATGATGAAAATGAATGAGGATGGCCTGACGCCTG1970.11544907933754497No Hit
GACCGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1970.11544907933754497No Hit
CGCTGGGGAGCCAAATCATGAGTGAACTCTCATTCACAGTTGCTACAAAGAG1950.11427700746609781No Hit
ACAACTGGGGGGAAAACTGGAAGAAGCACACCCCACTCTGATTATTCCACCA1940.11369097153037425No Hit
TTAAATGGGCTCTCCCTCCTGCCTAGCTAGGAAAGAGAAGAAAAAACTAAGC1930.11310493559465067No Hit
TATTACGGGGATTTCAAAATTTTCAGCTAGGAATGCAAAACTGAGAGAGAAA1920.11251889965892709No Hit
CATGTTGGCCAGGCTGGTCTCGAACTTCTGAGCTCAAGAGATCTGCCAGTCT1910.1119328637232035No Hit
AGATACGGGTGCGAGGGCATTATGAAGTTGTCACTCTCATAGCAGATGGCGG1890.11076079185175634No Hit
AACTGCGGGAGATCATGCTATCCCTAAGCAAAACAGACAGACAAACAAACAA1890.11076079185175634No Hit
TCAGACGGGCAAAAATTTCAGTGGTAACAGGTAAAATGATTCTACTACACAC1880.11017475591603278No Hit
TGTAGAGGGGAGAAAAATGGGGCATTTCAAAAAGGCTTAGACACTAATGGGA1880.11017475591603278No Hit
TACTGGGGGTGAGACACAAAAATCCATACAAAGAATCAACAAAACCAAAAGT1870.10958871998030918No Hit
GGTATAGGGACCACATTATAAATGAATGCTTGGATGTAAGGGCAGACCTGCA1860.10900268404458562No Hit
AACGTAGGGATAACCTGTAAGAGTATTCATTAAAACGTTCAACTTTTTCAAG1860.10900268404458562No Hit
TGGCCTCCCAAAGTGCTGGGATTATAGGAGTGAGCCACCATGCCCAGCCTGG1850.10841664810886205No Hit
ATTCCAGGGAGAAAATAGGGGTTAAGACTGATAACAACCTTTTACATTGTGA1840.10783061217313845No Hit
TGTGCTTGGGTACTAATGTGTACAGATCTTCTTTCTAAGGTGGTGAAAATAT1830.10724457623741489No Hit
CCGAATAGGCAGAGACTGTTCTAATTTTTATTACCTTGGAGTAATTTTCCTT1820.1066585403016913No Hit
TTGATAGGGCTGTTGTGACTTGGAACCAGTGAGAAGATGGCATGGGGAGGGG1810.10607250436596773No Hit
GTCGTAGGGGCATCCTCTGGGCAGCACCCAAGCCTTTCCACCTGTCCTGAGG1810.10607250436596773No Hit
ACTATAGGGAGGTGTGAGCCCCTATGCCTGGCCTTACTGTAGTTACATCTAA1810.10607250436596773No Hit
CGTAAAGGGTGGGAGAAGGTGCTTAACATGGCTGATTTTTTTTCTTCTTTTC1810.10607250436596773No Hit
TTAGGTGGGATAGCAGTAGTTAAGTATGGCTTACATAAGAGGATATTTTGCC1810.10607250436596773No Hit
TTAACTGGGGTCTTCATAATTTTTCAGTAGCTTCTTTCAATAGGAAACATTT1800.10548646843024415No Hit
GAGGTTGGGGTATTTTATGAATAAAAAATAAAATAATTTGTATTCATTGAGC1800.10548646843024415No Hit
GTTAGAGGGCTTCCGATCTGACCTGGGACGCATTCTGGTATCAGAATTTATT1800.10548646843024415No Hit
AACTATGGGTCTAGGAAAGGGAGGCTAGAAGAATCAAGTTGTCAGCATTGTT1800.10548646843024415No Hit
TAACTCGGGAATCTTAAATTTAGCTTGTCTTTCTGCTAAGCTGTTAAATGTA1800.10548646843024415No Hit
AGCCATGGGAGCCTATGTCATACAGCCTTGGTGCGTAGTAGGCAGTACCTTC1800.10548646843024415No Hit
GCTCACGGCAACACTTCTTTATGATAAAAACTCTCAGAAAAATAGTAATAGA1800.10548646843024415No Hit
CTCTTAGGGACGAGCAGCAAACCCAGCAGCTGAAACTGTCCCATGACAGGGA1790.10490043249452057No Hit
ACTAATGGGATTCCAAAGTGTCAGTTTTATGTGATAGTTAAAACAGTTTGTG1780.10431439655879698No Hit
TATACAGGGAGACTTCCCTTACTGCAAGCTGTTTTACCAGCTGAAGAATAAT1770.10372836062307339No Hit
GCGCTTGGGATTGACTTTCTTCACAGAATTGGAAAAAAAACTACTTCAAATT1760.10314232468734982No Hit
TCAGTAGGGGATCCCAGAATGTGTTGTGTTTTTTAAAAGATGCATAATAGCT1760.10314232468734982No Hit
GACTCAGGGAATGTATTGTTATTAACTGTAGTCAACGTGATGTACAATGGGT1760.10314232468734982No Hit
TTGTCCGGGAAGACTGTAGGTTTATGGGAGATATATAGTGGATTAGTTCTTC1760.10314232468734982No Hit
TGTCAGGGGGAGTTCAAGGCCAGCCTGGACAACATAGTAAGACCCTATCTCT1760.10314232468734982No Hit
TATTGCGGGGGGGAGAAAATATTTGTAAGTTATATATCTGATAAGGGATTAA1760.10314232468734982No Hit
TTGTGAGGGATTCCACTTAAACCACATCAACAGAGAATGGGTAAGATGTGTT1760.10314232468734982No Hit
CCCAATGGGAACTCATTGCCCTTCATGGAATCGCATGCCCGTTTCCCCCCAC1750.10255628875162626No Hit
ATGTACGGGATAATACAACTTAGTACATTAAAGCTACGAAAACTCATCCTGG1750.10255628875162626No Hit
TCATCAGGGATCTTTAGACTTTAGGCATCTTTCCATATTCCTCCAACAATTT1750.10255628875162626No Hit
AGCCGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1750.10255628875162626No Hit
ATCAGTGGGATTTTCAAACATTGTTCCTCGGACTGCTGAGAACTTCAGAGCA1740.10197025281590266No Hit
GTGGGTGGGATATTTCTTACTACAATATGATTTTCATTTACAGGCACTATTA1740.10197025281590266No Hit
GAGACGGGATAGTCACTGAAGACAGAAGTTTGGTATTTGGTATAGTCTACAA1730.1013842168801791No Hit
AGCAGTGGGGAGCCCAGGAATTTGAGGCTCCAGTGAGCTATGATTGTGCCAC1730.1013842168801791No Hit
GCACTTGGGCTTTTATCTTTTAACCAACTGAACAATACACCAAAAGCAGCCT1730.1013842168801791No Hit
ACTACAGGGATACAAAATGCTCTGATAATCATCATTGCATTATATTTTTATT1730.1013842168801791No Hit
TGTCTTGGGAGGCATTCGTTTATTGTTTTGTTCCTTGTATAAAAGATTTTAA1730.1013842168801791No Hit
TGACTCGGGGACACATTGTGTTCACGGAATGGAAAACTCACTGTTGTTGAGA1730.1013842168801791No Hit
TTCATGGGGCTTATTTCTAGCCATGAGTTTTGTGAGGAACCAGAGGCTACTT1730.1013842168801791No Hit
ACTCATGGGATCATCTCCCCATCTCCAGATCCTTAATATCATCACATCTGTG1730.1013842168801791No Hit
CTACTGGGTCCTAGTCTCTGATAAAACAGACTTTAAACCAACAAATATCAAA1730.1013842168801791No Hit
CACAGCGGGATGGGCTAAAGTAACAAAGACTAGAAAAACAGCCAATTTTCTA1720.10079818094445553No Hit
CGCTAAGGGATTCATGTGCAAACTGCAATTCATATACTCCAGGATTTGTCCC1720.10079818094445553No Hit
TTAAATGGGTACTGGAGGCAATTTCTAACACAATGACAAGTATTTGTGTCTC1720.10079818094445553No Hit
TATAAAGGGGCATGGCACAGTTTTGAACCTAATTCTGTGTTTTGCATTTTTA1720.10079818094445553No Hit
GTTAAAGGGCGATCTAGCTTAGGGGTAAATATTATGGTTACTTTTTATCTCA1710.10021214500873193No Hit
TCGTGCGGGTCATCAGCAAATAGAGCTCAGCTTTGTAGCTGCCTTTAAAATC1710.10021214500873193No Hit
GCAACAGGGAGACAAAATGCATACTTTATAGTTTCAAGATTTTCAGTATATA1710.10021214500873193No Hit
AGCTATGGGTAGAGCCACAGTCAACACATTTGGTTATATTGCAAAGGCCATT1710.10021214500873193No Hit
GATATTGGGAAGGAGCCTGGGCGTGGGAGGCCAGGACTGGGAAGGTTCTGGG1710.10021214500873193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)