FastQCFastQC Report
Sat 31 Dec 2022
SRR5845867.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845867.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93578
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC5770.6165979183141337No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT5440.5813332193464276No Hit
CTCGCAAAGGGGACCCATCTCATGTGCAAAGACACACATAGGCTCAAAATAA1480.15815683173395456No Hit
GGGATAGGGAGGGGTAGTTTACGCCCTAACTGTTCTTTTCTCCAGACACATA1450.15495095000961764No Hit
GCATAGGGGCAACACTCAAACACAGTTGCTGCACTTGGATTTTCCTTTAATA1440.153882322768172No Hit
GTGAACGGGAACTTGCACATGTTGTGCCATGCAGAAGGGATTTTTATGGACC1390.1485391865609438No Hit
TAGCCAGGGGTATAGCCAGAGAAAACAAGGATGGAGAAAAATCTAGGACAGT1370.14640193207805255No Hit
TGCAAAGGGTCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAG1310.13999016862937871No Hit
ACAAATGGGTTACTGGATTTGGTTTGCTACTACTTGTTGAGGATTTTTACAC1290.13785291414648743No Hit
AACTGGGGGAGGCATTAAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1280.13678428690504177No Hit
AGATTAGGGCGCTCTTCCGATCTACGAATGGGGCAGTTTGAATCGCGGTGCG1280.13678428690504177No Hit
GAGTTGGGGGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACG1260.1346470324221505No Hit
ATGCCGGGGAGCTGACGAATATGACCCTATGTTTCCTAATGATTATGAGAAA1250.13357840518070488No Hit
GTAATTGGGAGGAGGAATTCTTTCTTTATGGTAGAAAAGAACATCACTGAAA1250.13357840518070488No Hit
TTGTCGCGGGATGGCAGAAGATGGAAGGGCAAGGGAGAGCTAACATGCTCCC1240.13250977793925922No Hit
GTAGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1230.1314411506978136No Hit
GTGGGCGGGAAGGGTATCTATATTTAGATCAGAAAAAATAGACTTGAAATAA1220.13037252345636793No Hit
GCGAACGGGGTGTTTGCTTCCACAGCTCATGCAGCTGAAGCCATTTAAGTCT1220.13037252345636793No Hit
TCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGA1210.12930389621492233No Hit
AGCATCGGGGAGGATTTGCTTTGAGCTTTTAGAGCTCTTAAAAGCCCACAAA1210.12930389621492233No Hit
TTTTACGGGCTAAAAAATAAATGGTCAGTTCTGTCCTGGGTGAGTCACATTC1210.12930389621492233No Hit
CTATCCGGGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTC1200.12823526897347667No Hit
TTTATGGGGAGTCATTTAACCACTTGCATGAACAAAATTCAGCACCTTTCAG1200.12823526897347667No Hit
GCCATAGGGATCTCCTCCCATAACTCTTAACCTTGGATTGCCCCAGTGTCAG1200.12823526897347667No Hit
ACGCTTGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA1190.12716664173203104No Hit
AGACGCGGGCTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGA1180.1260980144905854No Hit
GACAAGGGGTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACA1170.12502938724913976No Hit
TAATGCGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG1170.12502938724913976No Hit
GTGCCAGGGAAATAATTCTGCTATGATTTCCTACTGAAGGAGATGCAAAGTT1170.12502938724913976No Hit
CCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGA1170.12502938724913976No Hit
TGACATGGGGGAGTTTCTAATAAAAACAAAAGCAGCCTTTTTTCTCATTATG1170.12502938724913976No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1170.12502938724913976No Hit
GACTCGCGGGCATGACATGGAAAGTCAACTGATTCTACAATTGGACATTCAT1160.12396076000769413No Hit
CGTCTTGGGACCTTTAAAATAAATACTATCGAAAGTCAAGAAGGTATTCCAC1150.12289213276624848No Hit
AATGTAGGGATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAGGCTT1140.12182350552480284No Hit
TCCTAAGGGATGTTGTCAGTAAGTAATAGGAATCTTTCAGCTCCCTAATAAT1140.12182350552480284No Hit
GCCAATGGGTAGAAACATGAAATTTTTAAAGAGAACCAAATCTAACATGAAA1130.1207548782833572No Hit
ATCACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1130.1207548782833572No Hit
GTGCGCTGGGACCATCAGACTTGGAAAATGGAAAGTATTTTATTGTCATGAT1120.11968625104191155No Hit
TGTCTAGGGTCCCCGGTCTAGACTGGAGCATTTGGTGCTTAATAATTTTTTT1110.11861762380046591No Hit
GGTACTGGGGGAATAAAAGGAAAAAGAAAAGATGGGAAATTTCTATAAGGAT1110.11861762380046591No Hit
AAATGAGGGCAGTCAGAGGCGCGGGGCGCAGGGCGCGCGGACGCATTGGAGG1100.11754899655902029No Hit
AGTTAGGGGTAACCTACCTTTACATTCTCAGTCAGAATTTGACCCAGAAAGA1100.11754899655902029No Hit
ACTTATGGGATGTGCTTTGCCAAAGATACTACCTTGTCATTTCTTCCAGCTT1100.11754899655902029No Hit
TGTGAGGGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCA1080.115411742076129No Hit
ATGGATGGGACTTCAAACTAAACTATAAGGCCTTAGACACCAAAACAGCATG1080.115411742076129No Hit
GATCCCGGGAGCCACTTTTTACAAATTGTATTTTCAGTTTTAGTCATTCTTT1080.115411742076129No Hit
GCAGCTGGGGCTTGTGTCAGGAGGGGTAGCAGAAGAAGCTAAACAAGCTCTT1080.115411742076129No Hit
GAACTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.115411742076129No Hit
AATGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.11434311483468336No Hit
TCCGCGGGGATGGGCCGTGTGATCCGTGGACAGAGGAAGGGCGCCGGGTCTG1070.11434311483468336No Hit
CTAATTGGGGGGCTGGAGCATCTTTGAGCTGGAAACCAGACTTCGAAATATG1070.11434311483468336No Hit
GTCTGCGGGGTGTGTTGCTTTGCAGCTGACTAGAAGCTCAGCATTTCCTAGC1060.11327448759323772No Hit
GTACAAGGGGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGA1060.11327448759323772No Hit
AGTACAGGGCAGTGGCCTGGGAAAGTTGACCAAACGATTGGTCAAGCAAACA1060.11327448759323772No Hit
CTCCAAGGGCTAAAATTGTGGCAGATAAAGATTATTCTGTGACCGCCAATTC1060.11327448759323772No Hit
TTCGCTGGGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTGTTGG1050.1122058603517921No Hit
CTATTTGGGATACGTACTTGGAATTGGGCTAGGATTTGAATACTAGGCTCTG1050.1122058603517921No Hit
TTAGTGGGGAGCAAAGATAAGGATAACATCTGTCTTCTCCTCGGAAACCATG1050.1122058603517921No Hit
GTTCACGGGGGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGA1050.1122058603517921No Hit
TTGCTAGGGCATAACACAATTTCCAAGAAATATATGTAAGATCATAATATGC1040.11113723311034646No Hit
TGACTAGGGTGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTAT1040.11113723311034646No Hit
ATAACCGGGTACTTCTTAGATTCCGTTAAGAATACTTTCTGGGGTGTGAGCC1040.11113723311034646No Hit
ATCTCAGGGAATTGGAAAAACAATAGAAAATTAATCCTAAAGAATGTAGAAG1030.11006860586890081No Hit
ATATTTGGGATAAATAGAAGAGGGACAGCTTACAGGCATTTTCATTATTTTT1030.11006860586890081No Hit
CTACCAGGGCACTAAACTCCACTACTAAATCTTTTTGAAAATAATACTGTGT1030.11006860586890081No Hit
CGCTCGGGGGCGTGACTGTGTCTGCTAGTGCCGGCATTGGGAATCTCGTGAC1030.11006860586890081No Hit
TAATTAGGGGTTTAAATAAACAATATAGTCAGTGGTCAAGGTACAGGCCTTA1030.11006860586890081No Hit
TACGTAAGGGATTTGTGATTGCAAGATAATTCGCTTGTGTATTGGATTCATT1020.10899997862745517No Hit
GCTCGTGGGGGTGTCAGGGTCTTTGCACTACTCTTCTCTCTGATCTTTACTT1020.10899997862745517No Hit
GAACATGGGAGTGGATGGATATTTTAAAAAATTCTGTTGTTCAAAACTCTTC1020.10899997862745517No Hit
GCACTGCGGGAGATCCCAACCGGGGCTTCCGGATTCTGTAGTGGCTTTGGCC1020.10899997862745517No Hit
AATGCGGGGGGTCTCTTAAACTTTTTCTCCACTATAATGCCATGGTCTTTAT1010.10793135138600952No Hit
GTGCAAGGGCTTGAATGTGATTGACCTACTTGAAAAATATTAATACCTTTAA1010.10793135138600952No Hit
ACGCCAGGGATCTCTGTAACAATTTAATGAGATAAAGCATGTAAACATGCTT1000.10686272414456391No Hit
TTAACAGGGTATCCATGCATTTTAATAATATAGTTATGGTCATGTTATCCCA1000.10686272414456391No Hit
ACGCAGGGGGTGACTGTGTACTGAGATACCACTAGCCCAGTTTGCTGGGTCT1000.10686272414456391No Hit
TCAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.10579409690311826No Hit
ATTGTGGGACCTTATTTTTGGTCTTTGTTTATTCATTTAGACCCTGCAAGTT990.10579409690311826No Hit
GACCTCGGGTAAAAAGTAAAAGTGTTCAAACTGTGATTGAATCTTTTTTGTG980.10472546966167262No Hit
CAGACTGGGGTCCAAAATATATCAAGAATAATGTACAAAGTACTCAACAATA980.10472546966167262No Hit
CAGCTAGGGGCCTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCT980.10472546966167262No Hit
TGGCCAGGGAACTTGAAGTGCATGTAATAGTGATGAGAGTAAGTAGCCAAAT970.10365684242022698No Hit
AATTAAGGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGA970.10365684242022698No Hit
AGCATGGGGGGTTTCAAAGAGAAAATGCAGACAATTTTGGTTTCAAAGAGAA970.10365684242022698No Hit
GCACACGGGATAACTAGAACTGAAAGCATTTTTAACATTCTTCTCCTGGAAG970.10365684242022698No Hit
GTTACAGGGAAATGTTCTTTTTTTAAAAAAGAACACAATCGAAATTATAGGC960.10258821517878133No Hit
CGAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA960.10258821517878133No Hit
TAGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC960.10258821517878133No Hit
AGCCAAGGGCTGTATTTGTGCTCTATGAAAGTTTCTGTATAACAGGCATTAA960.10258821517878133No Hit
CTGTAAGGGGTCGTGGGGATTTGCCGCATCAGCCGCATTGAATACCAGGGTT960.10258821517878133No Hit
TGCGCAAGGGACAGAGTGTAGCAGTAGGGAGTCTCTCACCGTCGCATGGTCC960.10258821517878133No Hit
ACACCCGGGATTAAACCCCGATCATAACCTCCAGCAGGCATTTCATTTAATA950.10151958793733569No Hit
CTAATAGGGGGGTGTATAGGGTTAGAGGAAGATTCTTTTTGTTTGAATTGTC950.10151958793733569No Hit
TACTGGGGCGCTTAAAATATGTCATGTACAACTCTTATAAACATTTTTACAG950.10151958793733569No Hit
CAGCCAGGGAGGAGACTCTTGCGTTAGTTCAGATAAAAACAATACAGATTTG950.10151958793733569No Hit
AAGCGGGACTCCAGCCTGGGCAACAGAATGAGACCCTGTCTCAAAAAATATG950.10151958793733569No Hit
AAAAGCGGGGACAGTTAAAGAGAATTGGGAAGGCTTCTGAGGAAGAGATGTT950.10151958793733569No Hit
TTCGATGGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA940.10045096069589007No Hit
TCTCATGGGGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTTTAAAGCAA940.10045096069589007No Hit
GCGACAGGGAGAGCAGCTGAGCACATCATTTCAGTGGGTCACCAAATGGGTT940.10045096069589007No Hit
AACTCTTCCTTCTATCTCTTCCCCACCTTTTTGATTTGATAAATTTCTAAGC940.10045096069589007No Hit
GTATCAGGGTCTGATTAAATTTTCTAGTTGCTTACGTAAATCATGAATTGGA940.10045096069589007No Hit
ATGCACGGGAGGCCCTTCTGACTTTGTTGCCCAGTGGCCAACGCCCGAGTGT940.10045096069589007No Hit
GTGGTAGGGGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACTTTT940.10045096069589007No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)