FastQCFastQC Report
Sat 31 Dec 2022
SRR5845866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91174
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT5430.5955645249742251No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC5100.5593699958321452No Hit
GTAGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1470.16123017526926536No Hit
CTATCCGGGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTC1440.15793976352907627No Hit
TTGTCGCGGGATGGCAGAAGATGGAAGGGCAAGGGAGAGCTAACATGCTCCC1420.15574615570228353No Hit
GCGAACGGGGTGTTTGCTTCCACAGCTCATGCAGCTGAAGCCATTTAAGTCT1390.15245574396209446No Hit
GTGAACGGGAACTTGCACATGTTGTGCCATGCAGAAGGGATTTTTATGGACC1340.14697172439511264No Hit
GCTCGTGGGGGTGTCAGGGTCTTTGCACTACTCTTCTCTCTGATCTTTACTT1280.14039090091473447No Hit
GGGATAGGGAGGGGTAGTTTACGCCCTAACTGTTCTTTTCTCCAGACACATA1280.14039090091473447No Hit
CGCGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1270.1392940970013381No Hit
GTAATTGGGAGGAGGAATTCTTTCTTTATGGTAGAAAAGAACATCACTGAAA1270.1392940970013381No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1230.13490688134775267No Hit
AGATTAGGGCGCTCTTCCGATCTACGAATGGGGCAGTTTGAATCGCGGTGCG1220.13381007743435627No Hit
GAGTTGGGGGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACG1220.13381007743435627No Hit
AATGTAGGGATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAGGCTT1200.13161646960756357No Hit
GCATAGGGGCAACACTCAAACACAGTTGCTGCACTTGGATTTTCCTTTAATA1200.13161646960756357No Hit
TAGCCAGGGGTATAGCCAGAGAAAACAAGGATGGAGAAAAATCTAGGACAGT1190.1305196656941672No Hit
ATATTGGGGTAGAGAAAAGTCTCAGTTTCACCAAATCCACATTCAAATGAGT1180.12942286178077084No Hit
AGCATCGGGGAGGATTTGCTTTGAGCTTTTAGAGCTCTTAAAAGCCCACAAA1170.12832605786737447No Hit
CTAAGACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1160.1272292539539781No Hit
TGCAAAGGGTCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAG1160.1272292539539781No Hit
TTTATGGGGAGTCATTTAACCACTTGCATGAACAAAATTCAGCACCTTTCAG1160.1272292539539781No Hit
CTCGCAAAGGGGACCCATCTCATGTGCAAAGACACACATAGGCTCAAAATAA1150.12613245004058174No Hit
CCATATGGGAGCCTCTCAAGTGACTGGGACTGCAGGCAAGTGCCATCATGCC1140.12503564612718537No Hit
ATGCCGGGGAGCTGACGAATATGACCCTATGTTTCCTAATGATTATGAGAAA1130.12393884221378901No Hit
ATAACCGGGTACTTCTTAGATTCCGTTAAGAATACTTTCTGGGGTGTGAGCC1130.12393884221378901No Hit
AGACGCGGGCTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGA1130.12393884221378901No Hit
GACAAGGGGTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACA1120.12284203830039266No Hit
TTTTACGGGCTAAAAAATAAATGGTCAGTTCTGTCCTGGGTGAGTCACATTC1110.12174523438699628No Hit
ACGCTTGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA1110.12174523438699628No Hit
CGTCTTGGGACCTTTAAAATAAATACTATCGAAAGTCAAGAAGGTATTCCAC1100.12064843047359994No Hit
AAGCATGTGCAAAGGAGAGGAGGGAGATGAGGTCATTGTTTGTCATTGAGTC1100.12064843047359994No Hit
GCACTGCGGGAGATCCCAACCGGGGCTTCCGGATTCTGTAGTGGCTTTGGCC1100.12064843047359994No Hit
ATCACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1090.11955162656020356No Hit
TTGCTGCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1090.11955162656020356No Hit
CGCTCGGGGGCGTGACTGTGTCTGCTAGTGCCGGCATTGGGAATCTCGTGAC1090.11955162656020356No Hit
ACACTGCGGGCAATTTAGTTTATGGATTTGGAGTAAACCATTTCAAGTCATT1070.11735801873341083No Hit
TCCGCGGGGATGGGCCGTGTGATCCGTGGACAGAGGAAGGGCGCCGGGTCTG1070.11735801873341083No Hit
GGATACGGGGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCCGGG1070.11735801873341083No Hit
TCCTAAGGGATGTTGTCAGTAAGTAATAGGAATCTTTCAGCTCCCTAATAAT1070.11735801873341083No Hit
GCTAGAGGGCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGACATGACTT1050.11516441090661811No Hit
GAACTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1050.11516441090661811No Hit
GTGGGCGGGAAGGGTATCTATATTTAGATCAGAAAAAATAGACTTGAAATAA1040.11406760699322176No Hit
AGCTAGGGCTTGAAGCCATTCTTACTAAATTATTAGTTTTGTAATTCGGTTT1040.11406760699322176No Hit
AACTGGGGGAGGCATTAAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.11406760699322176No Hit
ACAAATGGGTTACTGGATTTGGTTTGCTACTACTTGTTGAGGATTTTTACAC1030.11297080307982538No Hit
GAAATTGGGTGGAATGAGGTCATTGTGCCCGCAGGTTGTTTGTGATTAGCTG1030.11297080307982538No Hit
AATGCGGGGGGTCTCTTAAACTTTTTCTCCACTATAATGCCATGGTCTTTAT1030.11297080307982538No Hit
TCAGATGGGAACTTTTGACAAATTAGAGACAGAGACCCATTGGGTGCCAGTG1030.11297080307982538No Hit
AAAATTGGGGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTT1020.11187399916642903No Hit
TCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGA1020.11187399916642903No Hit
TTAGTGGGGAGCAAAGATAAGGATAACATCTGTCTTCTCCTCGGAAACCATG1020.11187399916642903No Hit
CATGCTGGGAAGAGGAAACCACCAAAGAAGAGTGGTCCCAAGACTTTAAAGA1010.11077719525303266No Hit
GTGCGCTGGGACCATCAGACTTGGAAAATGGAAAGTATTTTATTGTCATGAT1010.11077719525303266No Hit
AGCATGGGGGGTTTCAAAGAGAAAATGCAGACAATTTTGGTTTCAAAGAGAA1010.11077719525303266No Hit
ATACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1010.11077719525303266No Hit
TTCGCTGGGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTGTTGG1000.10968039133963631No Hit
AGCTAGGGGCAGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCGATTCTCC1000.10968039133963631No Hit
TATGCTGGGATGGAAAGGTGCCAAACTGTTTTGAATGGAAGGACAAATAAGA990.10858358742623993No Hit
CTCTAAGGGCAGTCTAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATC990.10858358742623993No Hit
GAGTCGGGTACTTCGATGCCTCGCCCTACCTGACCTTCGACGCCAAGAACCC990.10858358742623993No Hit
TTCTAGGGGATGTCATATTAGCCTGGTTATGACTATTCTCCTTTGCCACGTT990.10858358742623993No Hit
GCAGCTGGGGCTTGTGTCAGGAGGGGTAGCAGAAGAAGCTAAACAAGCTCTT990.10858358742623993No Hit
TAGCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC990.10858358742623993No Hit
CACAAGGGGGGCGGCGCTTCGACGGCGTCCAGGACCCGCGCATACTCATGGC990.10858358742623993No Hit
GCAGTTGGGCAGATAGCATGCCATTCATTTTTTTCTTTCTTTGTTCCTTTCC990.10858358742623993No Hit
AAGCGGGACTCCAGCCTGGGCAACAGAATGAGACCCTGTCTCAAAAAATATG990.10858358742623993No Hit
GAGCTTGGGAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTC980.10748678351284358No Hit
CGAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA980.10748678351284358No Hit
ACGCAGGGGGTGACTGTGTACTGAGATACCACTAGCCCAGTTTGCTGGGTCT980.10748678351284358No Hit
TATTATGGGCCAGCAGGCGCGGAGGATGGCGGGGAGGAAGCCAGCAGCCCCT980.10748678351284358No Hit
ATAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA970.10638997959944721No Hit
TCAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA970.10638997959944721No Hit
CTAATTGGGGGGCTGGAGCATCTTTGAGCTGGAAACCAGACTTCGAAATATG970.10638997959944721No Hit
TGTGAGGGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCA960.10529317568605086No Hit
GGTACTGGGGGAATAAAAGGAAAAAGAAAAGATGGGAAATTTCTATAAGGAT960.10529317568605086No Hit
ATCACTGGGGGAAGCCTCTGCTTTCTCTTCTTCCAACTCTTGGCCTTTCTAC960.10529317568605086No Hit
TTTGCAAGGGGATTAACTCATTCATGTAGTTTATTCAAGTGATTTATGTATT960.10529317568605086No Hit
CTCCAAGGGCTAAAATTGTGGCAGATAAAGATTATTCTGTGACCGCCAATTC960.10529317568605086No Hit
ATTCGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC950.10419637177265448No Hit
AATGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.10419637177265448No Hit
GACCTCGGGTAAAAAGTAAAAGTGTTCAAACTGTGATTGAATCTTTTTTGTG950.10419637177265448No Hit
TAGAGAGGGTGTGCTGCCTCCGTAGCCTCGCTGACCAACATCATCACTCCAG950.10419637177265448No Hit
TGCGCAAGGGACAGAGTGTAGCAGTAGGGAGTCTCTCACCGTCGCATGGTCC950.10419637177265448No Hit
ATCTCAGGGAATTGGAAAAACAATAGAAAATTAATCCTAAAGAATGTAGAAG940.10309956785925813No Hit
ATATTTGGGATAAATAGAAGAGGGACAGCTTACAGGCATTTTCATTATTTTT940.10309956785925813No Hit
CTACCAGGGCACTAAACTCCACTACTAAATCTTTTTGAAAATAATACTGTGT940.10309956785925813No Hit
ATATGAGGGCTGCTGCTGGCCTCGGGCATGGCCCGCGACTGGCCCGACGCCC940.10309956785925813No Hit
TAATGCGGGCCCTGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACG940.10309956785925813No Hit
CTATTTGGGATACGTACTTGGAATTGGGCTAGGATTTGAATACTAGGCTCTG940.10309956785925813No Hit
GACTCGCGGGCATGACATGGAAAGTCAACTGATTCTACAATTGGACATTCAT940.10309956785925813No Hit
CCTTTTGGGCTGAAACACTATGTATAATTAAAGTCTCTTGATCCAATATCAG940.10309956785925813No Hit
CAGTAAGGGCCTGAGATATTTTCTTAGCATCAGGCAATCACACAGGCTTGGA940.10309956785925813No Hit
TCTGCCGGGGAACCTGGAGCAAAGGAGACAAAGCAAGGTGGGTTTTGAACCT930.10200276394586175No Hit
TGGCCAGGGAACTTGAAGTGCATGTAATAGTGATGAGAGTAAGTAGCCAAAT930.10200276394586175No Hit
GCCATGGGGCTCCTGAAGCTGTATTTCCAGCTCTCACACATGTAATTTAGAC930.10200276394586175No Hit
CTGTCCCCAGCGGTGAGGTTGCAGTGAGTGAAGGAGAGGAGGTGATCTGTTC930.10200276394586175No Hit
AAGATAGGGCAAAGAAGGCCATTACATAATGGTAAAGGGATCAATTCAACAA930.10200276394586175No Hit
ATTGTGGGGTGGCCAGTGATGATGAGCATTTTTTCATGTGTCTTTTGACTGC930.10200276394586175No Hit
CCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGA930.10200276394586175No Hit
GAACATGGGAGTGGATGGATATTTTAAAAAATTCTGTTGTTCAAAACTCTTC930.10200276394586175No Hit
TAATTAGGGGTTTAAATAAACAATATAGTCAGTGGTCAAGGTACAGGCCTTA930.10200276394586175No Hit
GCCATAGGGATCTCCTCCCATAACTCTTAACCTTGGATTGCCCCAGTGTCAG930.10200276394586175No Hit
CCTGTTGGGCTGTCTCTACCTTCCAATGGGGTAACCACTATTAACAGTTGGG920.1009059600324654No Hit
AAATGAGGGCAGTCAGAGGCGCGGGGCGCAGGGCGCGCGGACGCATTGGAGG920.1009059600324654No Hit
TGTCTAGGGTCCCCGGTCTAGACTGGAGCATTTGGTGCTTAATAATTTTTTT920.1009059600324654No Hit
CTATGGGGGCTTCAAGGAAATAGATTGGAAGCAAAAGAAGGAGGGAAAAGAG920.1009059600324654No Hit
AAAAAAGGGGGTTGTCTGAACAGGGATTATAATTAGTTTACATACATACTCC920.1009059600324654No Hit
TAGCTAGGGTGGCATAATTTCTTGTTCTGTTGTGAATGCAGCTGCTGTAGTC920.1009059600324654No Hit
CAGCTAGGGGCCTTTCTTCCTTTTGGTGCGAGCTTGCTGTGGTTTTTGCTCT920.1009059600324654No Hit
ATTTCTGGGGGGGAACCATAGCAATTAGTCCACAGAACAAACGTTCTATTAG920.1009059600324654No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)