FastQCFastQC Report
Sat 31 Dec 2022
SRR5845865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91902
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC5520.600639811973624No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT5470.5951992339666167No Hit
GTGAACGGGAACTTGCACATGTTGTGCCATGCAGAAGGGATTTTTATGGACC1610.17518661182564035No Hit
CTATCCGGGATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTC1490.16212922460882243No Hit
GTAGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1440.15668864660181497No Hit
AGATTAGGGCGCTCTTCCGATCTACGAATGGGGCAGTTTGAATCGCGGTGCG1350.14689560618920153No Hit
ATGCCGGGGAGCTGACGAATATGACCCTATGTTTCCTAATGATTATGAGAAA1300.14145502818219408No Hit
AACTGGGGGAGGCATTAAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.1349263345737851No Hit
GCATAGGGGCAACACTCAAACACAGTTGCTGCACTTGGATTTTCCTTTAATA1230.13383821897238363No Hit
TTTATGGGGAGTCATTTAACCACTTGCATGAACAAAATTCAGCACCTTTCAG1230.13383821897238363No Hit
TCAGATGGGAACTTTTGACAAATTAGAGACAGAGACCCATTGGGTGCCAGTG1220.13275010337098214No Hit
GGGATAGGGAGGGGTAGTTTACGCCCTAACTGTTCTTTTCTCCAGACACATA1220.13275010337098214No Hit
GTAATTGGGAGGAGGAATTCTTTCTTTATGGTAGAAAAGAACATCACTGAAA1220.13275010337098214No Hit
GTTAGTGGGGCAAGAACCTCAAGGAGAGAACCCTGTACTGTGGCCCCTCTCG1200.13057387216817914No Hit
GAGTTGGGGGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACG1200.13057387216817914No Hit
GACAAGGGGTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACA1190.12948575656677763No Hit
GCGAACGGGGTGTTTGCTTCCACAGCTCATGCAGCTGAAGCCATTTAAGTCT1190.12948575656677763No Hit
TCTGGGTTCACCTCCCTGCTCATGGAACGTCTCTCAGTTGATTATGGCAAGA1180.12839764096537618No Hit
TAGCCAGGGGTATAGCCAGAGAAAACAAGGATGGAGAAAAATCTAGGACAGT1170.12730952536397466No Hit
AATGTAGGGATCCAGGTGAAAGAACTGGAGAAGCGTGCCTCAGGCCAGGCTT1170.12730952536397466No Hit
TGTCTAGGGTCCCCGGTCTAGACTGGAGCATTTGGTGCTTAATAATTTTTTT1170.12730952536397466No Hit
GCTCGTGGGGGTGTCAGGGTCTTTGCACTACTCTTCTCTCTGATCTTTACTT1140.1240451785597702No Hit
CTGTCCCCAGCGGTGAGGTTGCAGTGAGTGAAGGAGAGGAGGTGATCTGTTC1130.1229570629583687No Hit
AGTTAGGGGTAACCTACCTTTACATTCTCAGTCAGAATTTGACCCAGAAAGA1130.1229570629583687No Hit
AATGAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.1218689473569672No Hit
CCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGA1110.12078083175556573No Hit
ATTTCTGGGGGGGAACCATAGCAATTAGTCCACAGAACAAACGTTCTATTAG1110.12078083175556573No Hit
TTCGCTGGGGAAATAGATCGTTATGCCAGTGAAAATGTCAACAAATTGTTGG1100.11969271615416423No Hit
CTAAGACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1100.11969271615416423No Hit
CAATTTGGGGCAAGTGATTTATTTGCCAAGTGGTTCCGGGAAGCACTGGTAA1100.11969271615416423No Hit
ACACTGCGGGCAATTTAGTTTATGGATTTGGAGTAAACCATTTCAAGTCATT1100.11969271615416423No Hit
TATTATGGGCCAGCAGGCGCGGAGGATGGCGGGGAGGAAGCCAGCAGCCCCT1100.11969271615416423No Hit
ACAAATGGGTTACTGGATTTGGTTTGCTACTACTTGTTGAGGATTTTTACAC1090.11860460055276273No Hit
TTCTAGGGGATGTCATATTAGCCTGGTTATGACTATTCTCCTTTGCCACGTT1090.11860460055276273No Hit
GTTTTAGGGTGATAACTTTGGAATTCACACCGTTGGACTAGTTAAAAACTTC1080.11751648495136123No Hit
TTGTCGCGGGATGGCAGAAGATGGAAGGGCAAGGGAGAGCTAACATGCTCCC1070.11642836934995975No Hit
TGACTAGGGTGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTAT1070.11642836934995975No Hit
AAAAGCGGGGACAGTTAAAGAGAATTGGGAAGGCTTCTGAGGAAGAGATGTT1060.11534025374855825No Hit
GATCCCGGGAGCCACTTTTTACAAATTGTATTTTCAGTTTTAGTCATTCTTT1040.11316402254575525No Hit
CTAATTGGGGGGCTGGAGCATCTTTGAGCTGGAAACCAGACTTCGAAATATG1040.11316402254575525No Hit
TTTTACGGGCTAAAAAATAAATGGTCAGTTCTGTCCTGGGTGAGTCACATTC1040.11316402254575525No Hit
ATAACCGGGTACTTCTTAGATTCCGTTAAGAATACTTTCTGGGGTGTGAGCC1040.11316402254575525No Hit
GACCTCGGGTAAAAAGTAAAAGTGTTCAAACTGTGATTGAATCTTTTTTGTG1040.11316402254575525No Hit
ATACGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.11316402254575525No Hit
ACGCTTGGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAA1040.11316402254575525No Hit
AATGCGGGGGGTCTCTTAAACTTTTTCTCCACTATAATGCCATGGTCTTTAT1030.11207590694435378No Hit
GTGCAAGGGCTTGAATGTGATTGACCTACTTGAAAAATATTAATACCTTTAA1030.11207590694435378No Hit
AGCATGGGGGGTTTCAAAGAGAAAATGCAGACAATTTTGGTTTCAAAGAGAA1020.11098779134295228No Hit
AGCTAGGGCTTGAAGCCATTCTTACTAAATTATTAGTTTTGTAATTCGGTTT1020.11098779134295228No Hit
CGCGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1020.11098779134295228No Hit
AAGATGGGCTGCTTCTTTGACCATCACAAGAGAATTCAACTTTCTGTTTAAC1020.11098779134295228No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1020.11098779134295228No Hit
TTGCTAGGGCATAACACAATTTCCAAGAAATATATGTAAGATCATAATATGC1010.10989967574155078No Hit
ATAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.10989967574155078No Hit
TCAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.10989967574155078No Hit
GCAGTTGGGCAGATAGCATGCCATTCATTTTTTTCTTTCTTTGTTCCTTTCC1010.10989967574155078No Hit
GAACTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.10989967574155078No Hit
GCCATAGGGATCTCCTCCCATAACTCTTAACCTTGGATTGCCCCAGTGTCAG1010.10989967574155078No Hit
GCATGTGGGGAAAGCTATTACTTCTACACATAAAAATAACAAATGACATCAC1000.10881156014014928No Hit
TCTCATGGGGATGACGAACTGGCAACCAGGTTTCTTAAAAGTTTTAAAGCAA1000.10881156014014928No Hit
AGCTAGGGGCAGCTCACTGCAACCTCTGCCTCCTGGGTTCAAGCGATTCTCC1000.10881156014014928No Hit
AATTAAGGGGCGAGGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGA1000.10881156014014928No Hit
CTATGGGGGCTTCAAGGAAATAGATTGGAAGCAAAAGAAGGAGGGAAAAGAG1000.10881156014014928No Hit
TCCGCGGGGATGGGCCGTGTGATCCGTGGACAGAGGAAGGGCGCCGGGTCTG990.1077234445387478No Hit
ACGCTAGGGGGGCTCCACAAATGACAGTAATGGGCAGAAGCATTTCAGACAA990.1077234445387478No Hit
GCAGCTGGGGCTTGTGTCAGGAGGGGTAGCAGAAGAAGCTAAACAAGCTCTT990.1077234445387478No Hit
GTGGTAGGGGTCTTTTGGAAAACTACTTAAAGGGTGATTTTCTGTTACTTTT990.1077234445387478No Hit
GCCAATGGGTAGAAACATGAAATTTTTAAAGAGAACCAAATCTAACATGAAA980.1066353289373463No Hit
GACTCGCGGGCATGACATGGAAAGTCAACTGATTCTACAATTGGACATTCAT980.1066353289373463No Hit
GCTAGAGGGCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGACATGACTT980.1066353289373463No Hit
TGCAAAGGGTCCCAATGATGAGTTGCCATGCTAATACTGAGCCACCAGGTAG980.1066353289373463No Hit
ATCACTGGGGGAAGCCTCTGCTTTCTCTTCTTCCAACTCTTGGCCTTTCTAC980.1066353289373463No Hit
AAAAAAGGGGGTTGTCTGAACAGGGATTATAATTAGTTTACATACATACTCC980.1066353289373463No Hit
CTCGCAAAGGGGACCCATCTCATGTGCAAAGACACACATAGGCTCAAAATAA980.1066353289373463No Hit
CTTGCAGGGGGATGGCTCTGCGAGCAGGCCCAGGAGGAGCCGCAGCACGCTG970.1055472133359448No Hit
GTGCGCTGGGACCATCAGACTTGGAAAATGGAAAGTATTTTATTGTCATGAT970.1055472133359448No Hit
TAAACCGGGACAGATAAGATAACTCATATTGGAGAGAAATCTTACAAGTGTG970.1055472133359448No Hit
CTCTAAGGGCAGTCTAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATC970.1055472133359448No Hit
TCGCTGGGGGTACAACTTCTACTTCCACAAAATGTAGTTTGAGTTTTCCAGA970.1055472133359448No Hit
AAGCGGGACTCCAGCCTGGGCAACAGAATGAGACCCTGTCTCAAAAAATATG970.1055472133359448No Hit
AGACGCGGGCTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGA970.1055472133359448No Hit
AAAATTGGGGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTT960.1044590977345433No Hit
TATGCTGGGATGGAAAGGTGCCAAACTGTTTTGAATGGAAGGACAAATAAGA960.1044590977345433No Hit
ACTTATGGGATGTGCTTTGCCAAAGATACTACCTTGTCATTTCTTCCAGCTT960.1044590977345433No Hit
GGTACTGGGGGAATAAAAGGAAAAAGAAAAGATGGGAAATTTCTATAAGGAT960.1044590977345433No Hit
TTTGCAAGGGGATTAACTCATTCATGTAGTTTATTCAAGTGATTTATGTATT960.1044590977345433No Hit
CATTAGGGGAGCCTGGCTTCTACCCTCTACAAGCTTAAGAGGGAAAACTTAG960.1044590977345433No Hit
AATATGGGTCAGCCAAATGCCTCTCAGGTACCAGGCATGGTTCTAGGCACTA950.10337098213314183No Hit
GAAATTGGGTGGAATGAGGTCATTGTGCCCGCAGGTTGTTTGTGATTAGCTG950.10337098213314183No Hit
AGCATCGGGGAGGATTTGCTTTGAGCTTTTAGAGCTCTTAAAAGCCCACAAA950.10337098213314183No Hit
CTACCAGGGCACTAAACTCCACTACTAAATCTTTTTGAAAATAATACTGTGT950.10337098213314183No Hit
ATCACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA950.10337098213314183No Hit
GGATACGGGGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGCCGGG950.10337098213314183No Hit
TGTAATGGGAGAATAAAATTCTATAATGACAAAATATACATAAAAATTTTAC950.10337098213314183No Hit
GTCTGCGGGGTGTGTTGCTTTGCAGCTGACTAGAAGCTCAGCATTTCCTAGC940.10228286653174033No Hit
GTGGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC940.10228286653174033No Hit
CGGCTAGGGGGGCAACAGATGGTTTATTCAATAAGTGATTTTGAAGTAATGC940.10228286653174033No Hit
TGAATGGGGAAGCTATTATGAGTTTCCAAGAGGGACAATTAATGCATTTTCA940.10228286653174033No Hit
TAGAGAGGGTGTGCTGCCTCCGTAGCCTCGCTGACCAACATCATCACTCCAG940.10228286653174033No Hit
GAACATGGGAGTGGATGGATATTTTAAAAAATTCTGTTGTTCAAAACTCTTC940.10228286653174033No Hit
CTCCAAGGGCTAAAATTGTGGCAGATAAAGATTATTCTGTGACCGCCAATTC940.10228286653174033No Hit
TGTGAGGGGCACCTTTAACCCCCGCGCCCTGGCTCAGGGGCCCTGGTGGGCA930.10119475093033883No Hit
TTTAGCGGGGATCTGGTAATGGGGGGCCTGCAGTTACCTAAAACTCTTAAGG930.10119475093033883No Hit
CAGCATGGGACCAAAATGAAACAGCTTTGGAGTGATATCTTTAGGAGCCTGG930.10119475093033883No Hit
ATTTGTGGGAGGTGATTTAAAGTCTAAGCTACAATAATAAAAAGACCCTACA930.10119475093033883No Hit
ATGCATGGGAATTGAAAATGAAACATGTTCTTATTTTCCTTAAATTGAAGAA930.10119475093033883No Hit
GAGTTTGGGCGATAGAATAAAAACCAAATAACTTAGGGGAAAAGTGGGGTTA920.10010663532893735No Hit
TTAGTAGGGTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGAAGAA920.10010663532893735No Hit
ACATCGGGGGAAGAAGTGCGGAAGAACAAAGAATCCAAAGACCCTGCTGACG920.10010663532893735No Hit
AACTCTTCCTTCTATCTCTTCCCCACCTTTTTGATTTGATAAATTTCTAAGC920.10010663532893735No Hit
TGTACTGGGAGAAACCTTTTTTGAAAATTAAAAACATTAAGAAACTTCTTGT920.10010663532893735No Hit
TTGCTGCCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC920.10010663532893735No Hit
ATATTGGGGTAGAGAAAAGTCTCAGTTTCACCAAATCCACATTCAAATGAGT920.10010663532893735No Hit
AGCCTTGGGTCCGATCTCGGTTAGGGGGCTACTTTTAAAACTTTCAGTATTT920.10010663532893735No Hit
TCCTAAGGGATGTTGTCAGTAAGTAATAGGAATCTTTCAGCTCCCTAATAAT920.10010663532893735No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)