FastQCFastQC Report
Sat 31 Dec 2022
SRR5845861.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845861.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237601
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC14120.594273593124608No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT12300.5176745889116628No Hit
CTTTGCGGGGAACACAACTTGTTCAAACTACTCCGGTTATAGCACCCAAATT3320.1397300516411968No Hit
TAGCACGGGAATTTATTAAAGGAACAATTGTGATGTTTTATGGATTATTTAT3140.1321543259498066No Hit
TTCTAAGGGCTGCTGGATGGGCTTTTCTGAAAGTGACCTAGGCCAAGCCATG2960.12457860025841642No Hit
ATTCAGGGGTCTTAAAATTTGGAAGTTTCCACTTTTGCTAAGGTATGTTTTC2900.12205335836128635No Hit
CTAAACAAGGGGACCACATTACAATTAAGAATTTCTATTCAGCAGAGACAGC2900.12205335836128635No Hit
GTGCCGGGCCACAACGCCTGGCCTGATTTTATTTTTTATAGCTAATTGTATA2850.11994899011367798No Hit
GAAAGCTGGGATTAGAGAGGTGGTAAAAGATACTCATACAAACAAACCACAA2840.1195281164641563No Hit
CGTGCAGGGCACAATATCTTCCAAAAAATAGAGGCAGAGGCATTGCTTCCAA2800.1178446218660696No Hit
TTTGGTGGGCTGGAACCTCTTTTCTTCCCGAAAATGCTTATTTTTCTCTAAA2800.1178446218660696No Hit
CCTCTAGGGACAGTTCCAAGAGAAAACTGCATTCAACTTTATGCTATTAAAA2800.1178446218660696No Hit
TATTTAGGGTTATAAAATCCTTTTCATTTTGTACTGTTTGTATTATCTGTAG2780.11700287456702624No Hit
ACGAACGGGATTTTTAAAAGTGTATAAACTGTAAAGTATATTTTACTGGTGT2780.11700287456702624No Hit
CGTAATGGGATAGAATATCAAAATATTTATGGCCAGAAGTTCTGTCAATGTG2780.11700287456702624No Hit
GCATTGGGGGGGATTTTAAAAATCATTGAATAACTAGTTAAATGAAATTTTA2760.11616112726798287No Hit
TAGAAGGGATGCATTATTTGATTAAAATATACCATCTTGTTAGGTTTGTTTT2710.11405675902037449No Hit
ATAAATGGGACTGCTCAAAGACTTCACCTCCTAATAACATCAGATTGAGGAT2700.11363588537085283No Hit
AGATATGGCAATTAAGCTCGATAACATTTTCATAACCCCAAATGTTTCTTTG2700.11363588537085283No Hit
TAGTGGAGGGAATAGGAATTATGCAGGTACTCAGTATTTATTGTTTGAAGGC2690.11321501172133115No Hit
GTATCTGGGATAATATGAAGTTTTATTGCCTATCTATCTGAAGTGTCTAATT2690.11321501172133115No Hit
ATATCAGGGACTACAGTCTCCATGTCAAAGCATAATGTGTAGCATATCAGCA2680.11279413807180946No Hit
CTGTAAGGGAGGAACACTTTGATGCAAGCCATGTGGCCCTTATGGGTGGTTC2670.11237326442228779No Hit
GGACGCGGGTTATCTAAATATCTCAAGAAGACAAAAAGAAAATATTAAACCT2670.11237326442228779No Hit
GTTGGTGGGGGCCACAGAGCAAGACTCCGTCTCAAAAAAAGAAATGTTATGA2660.11195239077276611No Hit
CTGCCGGGGATTCAGCCTGGCATTTCTACCATAAGTTTTTGGTCTGCTGATT2650.11153151712324443No Hit
TGGTCTTGCCTGGTCGTTCTCTTTGGATTCAGTGCATGTCTCTACACACCCT2620.1102688961746794No Hit
TGCTTGGGCGAGAAAGCAATGGGTTATTCTATCTGTCAGAGTTAGGCAAGAA2620.1102688961746794No Hit
CATCAGGGTGAGATGAAGCGCTGATCCTGTAGTCACCTATTTTCGTACTGAA2610.10984802252515771No Hit
CGCTGGGATCCATATGCCTTTGCAATAACCAGATTGTGAAAAGCTAACAAGT2590.10900627522611438No Hit
ACACTAGGGATGGATTCTTCCTAATTTACTTAATCATTTCCTACTATAAGAC2580.1085854015765927No Hit
GCTTGTGGGATTGAATGACTGTTTCCCTTTCCTTCAAGGCCTAGAGTATATT2570.10816452792707101No Hit
TCTTATGGGCCCACAGGCCGGAGAGAGAGAGAAGCCAGAATCATGATCTCTT2560.10774365427754934No Hit
ATTGCAGGGTACGGATGGTCTGGCAACATGGAGAGAATCATGAAAGCACAAG2550.10732278062802766No Hit
AGATTAGGGCTGCAGAAGGATAATTTGCACATGGAGCTGTGATAACACTAAT2550.10732278062802766No Hit
TTCGTTGGGGATGTTATAAATTTGCTTGTGATATTTTGGGGAGCTGTAAGTT2540.10690190697850598No Hit
CTATCAGGGTATATGATTTGGACATTAACTCCTCATCAGATATAAGGTTTGC2530.1064810333289843No Hit
TTGAACGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2520.10606015967946263No Hit
GCAATAGGGATTTATATCAGGTGCTTCCATTTCAATTTTATCTTCAGCCAAA2520.10606015967946263No Hit
TAGTGAGGGATTTGTGAGAATATTTTCCACTTAGCCAGCTTTCTGCTCTGAG2520.10606015967946263No Hit
CAACTTGGGGTAGGCATAGTGTTCCCATCCTTTTCACATATATTCCTTTACA2510.10563928602994097No Hit
GGTAGGGGTATGTGATCAGTTGAAAAAATATGTATTCTGTGATTGTTGCAGA2510.10563928602994097No Hit
CGAACCGGGATATTTTTAAACCTCATATAATTAATTTCATACTAATTTTCAC2490.10479753873089759No Hit
ACTTGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA2490.10479753873089759No Hit
CGACGAGGGGACTAGCCAGTTTCTATGTTTTTGTTAGATATAAAATTAAATC2480.10437666508137593No Hit
CGCAACGGGAACCTAACCCCGGTTCTTGCTTATGGGAGATGCTGATTGTAGG2470.10395579143185425No Hit
GCAGCTGGGACCTTTTCCCTGCCTCTGACTTGGTATTTCCAGGTCCGTATTT2470.10395579143185425No Hit
ACACCGGGCTCACCACCTCTGTTAACATCTTAATCTAAGCCATTACTACCTT2460.10353491778233256No Hit
TAATCCGGGGGAGGCTGAGGCAGGAGAATCGCCTGAACCCAGGAGGCGGAGG2450.10311404413281089No Hit
GCATAAGGGATGAAATCTCACTGTGATATGATTTGAAGCTAGAAATGGTTCC2450.10311404413281089No Hit
AAATCAGGGAGGCGATCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATT2440.10269317048328921No Hit
AGTTCTGGGTCTGTGAGAAAGATGCTATCGTGATCCCGTTTTATAGAGAGGT2420.10185142318424585No Hit
TGTCAGGGGCGATCTGTATGAGGGAGGAAGGCATTGGTATTACGGAAGGTCC2420.10185142318424585No Hit
TTGCACGGGAAAATATGGTTGCATTCTGTGCATTTGGGCAAAATGAGCCAAT2420.10185142318424585No Hit
GAAATGGGGAAGTCTGTCCTTGGATTCCTTTCTCCTGAATCACAGCCTGGAG2420.10185142318424585No Hit
GTGACTGGGCGATCTATTTCAGGGATAATGGTAAAGGGATCAATTCAACAAG2410.10143054953472418No Hit
TGGACCGGGAGGAACTTAATAAATATAACTCAAAGGCTGTGATGCTTTATTT2410.10143054953472418No Hit
CGCAAGGGGACTTTTACATAATCGACTCATTAATGTTTATTGAGTGAAAGTG2410.10143054953472418No Hit
CTAGCAGGGATGTAAAGTGTGTGAGGAAACCCTTGCTTATAGCGTGGATTCC2390.10058880223568081No Hit
AACTGGGGGAACTGGACCACTGAGCTCAGGACAGAGCCCATTAAAGAAGAGG2390.10058880223568081No Hit
TTCGAACGGGATAGTGGGCACTTGATACACTTTAAATGAGTAAAGATGCATA2390.10058880223568081No Hit
TTTATTGGGTAGTTCTGTTTTCTTTAAAATTCATTGGAAACTTAAAGGTGCT2390.10058880223568081No Hit
GTGTTTGGGGAAAAGAAGAAAAAAACAAGCAAGGGTGAAAATGTGGGTAAAT2380.10016792858615914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)