FastQCFastQC Report
Sat 31 Dec 2022
SRR5845860.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845860.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences232672
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC14180.6094416173841287No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT11420.49081969467748593No Hit
CTTTGCGGGGAACACAACTTGTTCAAACTACTCCGGTTATAGCACCCAAATT3330.14311992848301472No Hit
GTGCCGGGCCACAACGCCTGGCCTGATTTTATTTTTTATAGCTAATTGTATA3230.13882203273277405No Hit
TAGCACGGGAATTTATTAAAGGAACAATTGTGATGTTTTATGGATTATTTAT3100.13323476825746114No Hit
TAGAAGGGATGCATTATTTGATTAAAATATACCATCTTGTTAGGTTTGTTTT3000.12893687250722047No Hit
TATTTAGGGTTATAAAATCCTTTTCATTTTGTACTGTTTGTATTATCTGTAG2810.12077087058176315No Hit
TTCTAAGGGCTGCTGGATGGGCTTTTCTGAAAGTGACCTAGGCCAAGCCATG2800.1203410810067391No Hit
GTATCTGGGATAATATGAAGTTTTATTGCCTATCTATCTGAAGTGTCTAATT2800.1203410810067391No Hit
ACGAACGGGATTTTTAAAAGTGTATAAACTGTAAAGTATATTTTACTGGTGT2750.11819213313161876No Hit
TTTGGTGGGCTGGAACCTCTTTTCTTCCCGAAAATGCTTATTTTTCTCTAAA2740.11776234355659468No Hit
ATTCAGGGGTCTTAAAATTTGGAAGTTTCCACTTTTGCTAAGGTATGTTTTC2740.11776234355659468No Hit
CGTAATGGGATAGAATATCAAAATATTTATGGCCAGAAGTTCTGTCAATGTG2720.11690276440654654No Hit
CATCAGGGTGAGATGAAGCGCTGATCCTGTAGTCACCTATTTTCGTACTGAA2710.11647297483152248No Hit
TGCTTGGGCGAGAAAGCAATGGGTTATTCTATCTGTCAGAGTTAGGCAAGAA2690.11561339568147434No Hit
TGGCAGGGATGCTGATGGTAATGGCACAATTGACTTCCCTGAATTTCTGACA2670.1147538165314262No Hit
TAATCCGGGGGAGGCTGAGGCAGGAGAATCGCCTGAACCCAGGAGGCGGAGG2660.11432402695640215No Hit
ATATCAGGGACTACAGTCTCCATGTCAAAGCATAATGTGTAGCATATCAGCA2660.11432402695640215No Hit
GCTTGTGGGATTGAATGACTGTTTCCCTTTCCTTCAAGGCCTAGAGTATATT2650.11389423738137808No Hit
CTCACACGGGGAGTGGGCGGAAGAGGTGGCCCCGGATGCTGCGGCGCCCGCT2650.11389423738137808No Hit
GCCCACGGATTGCCACAGAGTTCATTGCTCTCAGTATAAGATTTTACTTTAT2620.11260486865630587No Hit
ATAAATGGGACTGCTCAAAGACTTCACCTCCTAATAACATCAGATTGAGGAT2600.11174528950625773No Hit
TTTATTGGGTAGTTCTGTTTTCTTTAAAATTCATTGGAAACTTAAAGGTGCT2600.11174528950625773No Hit
GAAAGCTGGGATTAGAGAGGTGGTAAAAGATACTCATACAAACAAACCACAA2570.11045592078118553No Hit
TTCGAACGGGATAGTGGGCACTTGATACACTTTAAATGAGTAAAGATGCATA2560.11002613120616146No Hit
CAACTTGGGGTAGGCATAGTGTTCCCATCCTTTTCACATATATTCCTTTACA2550.10959634163113739No Hit
ACTCGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2540.10916655205611332No Hit
TAGTGGAGGGAATAGGAATTATGCAGGTACTCAGTATTTATTGTTTGAAGGC2530.10873676248108925No Hit
GCAGCTGGGACCTTTTCCCTGCCTCTGACTTGGTATTTCCAGGTCCGTATTT2530.10873676248108925No Hit
GCAATAGGGATTTATATCAGGTGCTTCCATTTCAATTTTATCTTCAGCCAAA2530.10873676248108925No Hit
CGAACCGGGATATTTTTAAACCTCATATAATTAATTTCATACTAATTTTCAC2520.1083069729060652No Hit
CTGCCGGGGATTCAGCCTGGCATTTCTACCATAAGTTTTTGGTCTGCTGATT2520.1083069729060652No Hit
CTAAACAAGGGGACCACATTACAATTAAGAATTTCTATTCAGCAGAGACAGC2500.10744739375601706No Hit
GCATTGGGGGGGATTTTAAAAATCATTGAATAACTAGTTAAATGAAATTTTA2490.10701760418099299No Hit
AGATTAGGGCTGCAGAAGGATAATTTGCACATGGAGCTGTGATAACACTAAT2490.10701760418099299No Hit
GGTAGGGGTATGTGATCAGTTGAAAAAATATGTATTCTGTGATTGTTGCAGA2480.10658781460596892No Hit
GGACGCGGGTTATCTAAATATCTCAAGAAGACAAAAAGAAAATATTAAACCT2480.10658781460596892No Hit
GTTACTGGGAAGGCAGTTTCAGATCGCCACCTTTTGAGGTACATTTCTTTAA2460.10572823545592078No Hit
CCCAGGGGGGTCCACTGTAGAATGACTTATTTATGGCATGAGATACCTGGAA2450.10529844588089671No Hit
CGCAACGGGAACCTAACCCCGGTTCTTGCTTATGGGAGATGCTGATTGTAGG2440.10486865630587264No Hit
TGGACCGGGAGGAACTTAATAAATATAACTCAAAGGCTGTGATGCTTTATTT2430.10443886673084858No Hit
TAGCATGGGAAAACAAACCATATCCAGACCACAGCAGTCACACCCACTTTCT2420.10400907715582451No Hit
GCTTTTGGGATGATGGTTAAGACAAAAACTGAGTTATCCATCTCCTTTTACT2420.10400907715582451No Hit
ATTTTTGGGGAACATTCATGAGAAATATCCCTGCAGGTTAATGGTGTTTATT2420.10400907715582451No Hit
TGGTCTTGCCTGGTCGTTCTCTTTGGATTCAGTGCATGTCTCTACACACCCT2410.10357928758080044No Hit
CCTCTAGGGACAGTTCCAAGAGAAAACTGCATTCAACTTTATGCTATTAAAA2400.10314949800577637No Hit
CGCTGGGATCCATATGCCTTTGCAATAACCAGATTGTGAAAAGCTAACAAGT2400.10314949800577637No Hit
CGACGAGGGGACTAGCCAGTTTCTATGTTTTTGTTAGATATAAAATTAAATC2400.10314949800577637No Hit
ACACTAGGGATGGATTCTTCCTAATTTACTTAATCATTTCCTACTATAAGAC2390.1027197084307523No Hit
CGTGCAGGGCACAATATCTTCCAAAAAATAGAGGCAGAGGCATTGCTTCCAA2380.10228991885572823No Hit
AGATATGGCAATTAAGCTCGATAACATTTTCATAACCCCAAATGTTTCTTTG2380.10228991885572823No Hit
GCAAAAGGGGGAATCACCACCTTGTTAATGAACATCAAAATCTTTTGTGTCA2360.10143033970568009No Hit
GTGTTTGGGGAAAAGAAGAAAAAAACAAGCAAGGGTGAAAATGTGGGTAAAT2360.10143033970568009No Hit
TCATGTGGGAGTCTTCTGTTTTAAGTCATGTGATAACATCTCCCCAAACATC2360.10143033970568009No Hit
TTCAATGGGACACTGTGGAAGCTGAAGTAATGTACTTTCATCTGAAACAGAC2350.10100055013065604No Hit
GATTTAGGGGTTTGGAAGAAAGTTCTACCCATCTTCTCTTACACTCATTCAA2350.10100055013065604No Hit
TTGAACGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2350.10100055013065604No Hit
TTTCCAGGGGGACTAAAGACAGATAGAGGAAGAGAAAGAGGCTATTCAAACA2350.10100055013065604No Hit
GTGACTGGGCGATCTATTTCAGGGATAATGGTAAAGGGATCAATTCAACAAG2350.10100055013065604No Hit
TAGTGAGGGATTTGTGAGAATATTTTCCACTTAGCCAGCTTTCTGCTCTGAG2340.10057076055563197No Hit
TTGGGATGGGGCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGGGC2330.1001409709806079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)