FastQCFastQC Report
Sat 31 Dec 2022
SRR5845859.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845859.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230675
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC14610.6333586214370868No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT11640.5046060474693833No Hit
CTTTGCGGGGAACACAACTTGTTCAAACTACTCCGGTTATAGCACCCAAATT3730.16169936057223366No Hit
GTGCCGGGCCACAACGCCTGGCCTGATTTTATTTTTTATAGCTAATTGTATA3030.1313536360680611No Hit
GAAAGCTGGGATTAGAGAGGTGGTAAAAGATACTCATACAAACAAACCACAA2800.12138289801669015No Hit
ACGAACGGGATTTTTAAAAGTGTATAAACTGTAAAGTATATTTTACTGGTGT2720.11791481521621328No Hit
GTATCTGGGATAATATGAAGTTTTATTGCCTATCTATCTGAAGTGTCTAATT2710.11748130486615369No Hit
TAGCACGGGAATTTATTAAAGGAACAATTGTGATGTTTTATGGATTATTTAT2710.11748130486615369No Hit
TAGAAGGGATGCATTATTTGATTAAAATATACCATCTTGTTAGGTTTGTTTT2680.11618077381597486No Hit
AGATTAGGGCTGCAGAAGGATAATTTGCACATGGAGCTGTGATAACACTAAT2680.11618077381597486No Hit
TTCGAACGGGATAGTGGGCACTTGATACACTTTAAATGAGTAAAGATGCATA2660.11531375311585564No Hit
GAGCATGGGATTCATTTTGCTTTTAAAAGAATCTGTTAGTTAAGGACAGAAT2650.11488024276579603No Hit
GCATTGGGGGGGATTTTAAAAATCATTGAATAACTAGTTAAATGAAATTTTA2610.1131462013655576No Hit
TTTAACGGGATTTACCTTCCTTTGTCTTACATTGGCTTTTTAAATTTTCCAT2590.11227918066543839No Hit
TCACTTGGGTTATCTTTTTTTAAAAGAAAGATTTTCCAGCTAAAACAGAGTT2580.11184567031537879No Hit
TTCTAAGGGCTGCTGGATGGGCTTTTCTGAAAGTGACCTAGGCCAAGCCATG2570.11141215996531917No Hit
TAGTGGAGGGAATAGGAATTATGCAGGTACTCAGTATTTATTGTTTGAAGGC2560.11097864961525955No Hit
TCCTTGGGGATCTGATGATCTAAAAATCAAAATCACTTTGAAAGGCTTAAGT2550.11054513926519996No Hit
TTGAACGGGGGAACTTGGCTCAGCTGCCGGGCTGCTCCGGTTGGAAACGCCA2540.11011162891514034No Hit
CGCTGGGATCCATATGCCTTTGCAATAACCAGATTGTGAAAAGCTAACAAGT2540.11011162891514034No Hit
CCTCTAGGGACAGTTCCAAGAGAAAACTGCATTCAACTTTATGCTATTAAAA2500.10837758751490191No Hit
CTAAACAAGGGGACCACATTACAATTAAGAATTTCTATTCAGCAGAGACAGC2490.10794407716484232No Hit
TGCTTGGGCGAGAAAGCAATGGGTTATTCTATCTGTCAGAGTTAGGCAAGAA2490.10794407716484232No Hit
ATTCGCGGGATTGTACATATTTTTATATTCTCTTTATAAATGCATGTCTGAT2480.1075105668147827No Hit
CAACCAGGGTGTGCTAATATATGAATTCTTGATATAGTTGGTGCCAAAGTTT2480.1075105668147827No Hit
GAGGCAGGAGAATCGCCTGAACCCAGGAAGCAGATGTTGTACCGAGCTGAGA2480.1075105668147827No Hit
GTATGCACGGGTACTAACTAAGTTAAATTTAAAAGCAAGGAGAAGCATCATT2460.10664354611466349No Hit
GCAAGTGGGCATTTTTAAATTTCTTCTGAATTGTTTTTTAATTATAATACAA2460.10664354611466349No Hit
ATTATAGGGAAGGAGCCCCAGTGGCAGTGCAGAGAGACTATGGTTTTTGGTG2450.10621003576460387No Hit
TTCGTTGGGGATGTTATAAATTTGCTTGTGATATTTTGGGGAGCTGTAAGTT2450.10621003576460387No Hit
ATTAACGGGCTCTCAAACATTGATGGTGGAAGTGGGAATTGGTACAACTTTG2430.10534301506448465No Hit
CGTAATGGGATAGAATATCAAAATATTTATGGCCAGAAGTTCTGTCAATGTG2430.10534301506448465No Hit
TATGCCGGAGGGCTGTTTCAAGGAAATCTATTAAAGAGAAACGAAATACAAA2420.10490950471442506No Hit
CATCAGGGTGAGATGAAGCGCTGATCCTGTAGTCACCTATTTTCGTACTGAA2410.10447599436436544No Hit
TGTAATGGGATGCACTCTCATTGCAACTTCATAGTTTCTAACATTTATTCTT2410.10447599436436544No Hit
ATAAATGGGACTGCTCAAAGACTTCACCTCCTAATAACATCAGATTGAGGAT2410.10447599436436544No Hit
ATTGCAGGGTACGGATGGTCTGGCAACATGGAGAGAATCATGAAAGCACAAG2410.10447599436436544No Hit
ATATCAGGGACTACAGTCTCCATGTCAAAGCATAATGTGTAGCATATCAGCA2410.10447599436436544No Hit
TTTGGTGGGCTGGAACCTCTTTTCTTCCCGAAAATGCTTATTTTTCTCTAAA2400.10404248401430585No Hit
GGACGCGGGTTATCTAAATATCTCAAGAAGACAAAAAGAAAATATTAAACCT2400.10404248401430585No Hit
GGTAGGGGTATGTGATCAGTTGAAAAAATATGTATTCTGTGATTGTTGCAGA2390.10360897366424623No Hit
ATTCAGGGGTCTTAAAATTTGGAAGTTTCCACTTTTGCTAAGGTATGTTTTC2390.10360897366424623No Hit
TCTGTCGGGAGCACTGAAGTGTGCCAGGCAAGGACTGTGGATTTCAAGAACT2380.10317546331418664No Hit
CTGCCGGGGATTCAGCCTGGCATTTCTACCATAAGTTTTTGGTCTGCTGATT2380.10317546331418664No Hit
GCTTGTGGGATTGAATGACTGTTTCCCTTTCCTTCAAGGCCTAGAGTATATT2370.10274195296412703No Hit
TAGCTAGGGGTGCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTAC2370.10274195296412703No Hit
TTTATTGGGTAGTTCTGTTTTCTTTAAAATTCATTGGAAACTTAAAGGTGCT2370.10274195296412703No Hit
TTGTTGGGGCAGTTTTCTTGTTTGGGTTGCCAGAATATCAGGAAGATATTAA2360.10230844261406741No Hit
ACAACCGGGTCCTGCAAACTAAGAATCACTAACGTGGATGTATAATTCTGTT2360.10230844261406741No Hit
GTTGGTGGGGGCCACAGAGCAAGACTCCGTCTCAAAAAAAGAAATGTTATGA2360.10230844261406741No Hit
CGACGAGGGGACTAGCCAGTTTCTATGTTTTTGTTAGATATAAAATTAAATC2330.1010079115638886No Hit
ATTTCCGGGTATACTTGAGGGTTTTATCCTTTATTTTGGGGGATTTTTTGTT2320.10057440121382898No Hit
TACCATGGGAGCCAGCGCAATCAGAGAAGAGAAAGAAATAAAGGGCATCCAA2320.10057440121382898No Hit
GCAGCTGGGACCTTTTCCCTGCCTCTGACTTGGTATTTCCAGGTCCGTATTT2320.10057440121382898No Hit
TAATCCGGGGGAGGCTGAGGCAGGAGAATCGCCTGAACCCAGGAGGCGGAGG2320.10057440121382898No Hit
CATACACGGGTATAGTCGGGAACTAGAAAGAATTTGGAGACCATCTAACTGA2310.10014089086376939No Hit
GCCCACGGATTGCCACAGAGTTCATTGCTCTCAGTATAAGATTTTACTTTAT2310.10014089086376939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)