FastQCFastQC Report
Sat 31 Dec 2022
SRR5845849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166778
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT13130.7872741009005985No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC10790.6469678254925709No Hit
CCTGAGGTCAAAAGAGAAAAATTAACATATTGAACTAAAAACATGTCTCATA2690.161292256772476No Hit
CAAGACATGGAACATTATCTCATTCCAGAAAGATCCTTCATGTCCCTCTTTA2470.14810106848625118No Hit
TACCTGGGTTGCCTACTTTAATTATATGAAATACCAGGAGGTCAGATGTTTA2230.13371068126491503No Hit
CAGCCCAGGGATGTGTTTGGTTTGGAAATCAAGGCCTATAATAAATCACTGA2180.13071268392713667No Hit
AATGTGGGGGAACTTTCCCAACCAGCAGAACCATGAGCCAAATAAACCTCAT2100.12591588818669128No Hit
AGTAGCGGGGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGG1960.11752149564091187No Hit
TGCTAGGGAGTGGGCGGACCGCGCGGCTGGAGGTGTGAGGATCCGAACCCAG1960.11752149564091187No Hit
CATTTAGGGGTGGTAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTA1950.1169218961733562No Hit
CTTTGGGGGCTGCTGGTAGATGAGAAAATCTAGGATTAGGAAACACCTTGTA1890.11332429936802217No Hit
ATTTAAGGGGAAGAACCGCCAGCTAAACTTTGTAAAATTCTTGACAAGAGCC1870.11212510043291082No Hit
GCATATGGGTGCCTGAAGGTTCTGTGAACTCCTTAAGCGTTTGAATGTAAAT1870.11212510043291082No Hit
ACTTGGGGGTCCGATCTTCGAATGGGAATAAAATAAAAGAATAAATAAAGAA1850.11092590149779948No Hit
GTTAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1850.11092590149779948No Hit
TATTGCGGGAATTAAGACCATAATTTGGAGAGATAAGAGAAAGAATTTGCCC1830.10972670256268813No Hit
TTTACTGGGAGGTGAGGTTTCCATGGACCTTCAAAGGTGCCAGCAGCCTGCA1830.10972670256268813No Hit
TAGCGGGGGGTAGTTGAAATAGAACAAACATGGCTATATTAATATCAGACAA1800.1079279041600211No Hit
GTTATTGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCCT1780.10672870522490975No Hit
CGACCCGGCCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATT1770.10612910575735408No Hit
TACGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1740.10433030735468707No Hit
TAAAACGGGGATATACTGCTTACAAGAAACCCACTTGGAATATAATAACCAA1730.10373070788713139No Hit
GAGCGAGGGGTCCAAGATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAG1720.10313110841957572No Hit
GTCATAGGGCATCACTAAAAATTTGTTCCTTTTTCACTATGGGCAGTTCACA1710.10253150895202004No Hit
TCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.10193190948446437No Hit
GGTCAGGGTTATCTGGCAAGCCCAGTTAGTACTTGAGTGACTTAAAAGCCAG1700.10193190948446437No Hit
ACGGGCGGGGACACATGAACCAGACACATAAGTCACATAAATGAGCAAGAGG1690.1013323100169087No Hit
ACACATGGGGTATGACATAAATAAAGATGGCTACATCACTAAAGAGGAAATG1680.10073271054935302No Hit
GTTACGGGGGCTCCACATCCAGGTCAGGTGGCGTTTGCTGTGGCGGCTAGGC1670.10013311108179736No Hit
CGCACTGGGGAAGAGCAAACCAGGTAAACCTTTTGATTCTCCTAAATCAGAT1670.10013311108179736No Hit
TTAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1670.10013311108179736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)