FastQCFastQC Report
Sat 31 Dec 2022
SRR5845848.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845848.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161475
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT14020.8682458584920265No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC11030.6830778758321722No Hit
CAAGACATGGAACATTATCTCATTCCAGAAAGATCCTTCATGTCCCTCTTTA2480.1535841461526552No Hit
CCTGAGGTCAAAAGAGAAAAATTAACATATTGAACTAAAAACATGTCTCATA2430.15048769159312586No Hit
AGTAGCGGGGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGG2380.14739123703359655No Hit
CAGCCCAGGGATGTGTTTGGTTTGGAAATCAAGGCCTATAATAAATCACTGA1950.12076172782164421No Hit
TACCTGGGTTGCCTACTTTAATTATATGAAATACCAGGAGGTCAGATGTTTA1930.1195231459978325No Hit
CATTTAGGGGTGGTAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTA1870.11580740052639726No Hit
TCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1860.11518810961449141No Hit
TGATAAGGGGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGATAGAGA1840.11394952779067967No Hit
CGACCCGGCCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATT1830.1133302368787738No Hit
AATGTAGGGCCGATCTTACGTGGGGCCGATCTGCCGTGGGGATCTTTTAAGT1830.1133302368787738No Hit
TACGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1820.11271094596686793No Hit
AATGTGGGGGAACTTTCCCAACCAGCAGAACCATGAGCCAAATAAACCTCAT1810.11209165505496206No Hit
TTTCGCAGGGGGGATGGCCGCCACAGCTGTAGGTGCTGCTAGTGTTTAGCGC1800.11147236414305621No Hit
ATTTAAGGGGAAGAACCGCCAGCTAAACTTTGTAAAATTCTTGACAAGAGCC1780.11023378231924447No Hit
GGTCAGGGTTATCTGGCAAGCCCAGTTAGTACTTGAGTGACTTAAAAGCCAG1770.1096144914073386No Hit
GTTATTGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCCT1770.1096144914073386No Hit
TTTACTGGGAGGTGAGGTTTCCATGGACCTTCAAAGGTGCCAGCAGCCTGCA1730.10713732775971513No Hit
GTTTGCGGGATGAACAGACACTTTTCAAAAGAAGACATTTATGCAGCCAACA1730.10713732775971513No Hit
TTAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1710.10589874593590339No Hit
AATGCGGGGGTCCATGTTTCTCACATTGCTGTATTTTAGAAAATAGGGGTTA1670.10342158228827993No Hit
CTTTGGGGGCTGCTGGTAGATGAGAAAATCTAGGATTAGGAAACACCTTGTA1640.10156370955256232No Hit
CTATAAGGGACCGCAAAACTCCTACAGCAGAAGTTGAACGTGGGCAAGGTCC1620.10032512772875059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)