FastQCFastQC Report
Sat 31 Dec 2022
SRR5845847.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845847.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161935
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT12280.7583289591502763No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC11480.7089264210948838No Hit
CCTGAGGTCAAAAGAGAAAAATTAACATATTGAACTAAAAACATGTCTCATA2220.13709204310371445No Hit
CAAGACATGGAACATTATCTCATTCCAGAAAGATCCTTCATGTCCCTCTTTA2220.13709204310371445No Hit
CAGCCCAGGGATGTGTTTGGTTTGGAAATCAAGGCCTATAATAAATCACTGA2020.1247414085898663No Hit
TACCTGGGTTGCCTACTTTAATTATATGAAATACCAGGAGGTCAGATGTTTA2010.1241238768641739No Hit
AGTAGCGGGGATGGGGCCTCCCCAGAAGAGCAGCTTCTTCTCGTTGAGAAGG1910.11794855960724983No Hit
AGAATCGGGTCTTAAATTATTTTCTAAATATCTACTCCAGAATGTCATAAAA1910.11794855960724983No Hit
AGTGCCGGGAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTA1890.116713496155865No Hit
CGACCCGGCCCAGTTGCAGGTAATTTTTTGTCTGCTAGTAGTGTTCTAGATT1860.11486090097878779No Hit
CATTTAGGGGTGGTAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTA1770.10930311544755612No Hit
GAGCGAGGGGTCCAAGATGGCGGATGAAGCCACGCGACGTGTTGTGTCTGAG1760.10868558372186371No Hit
GCATATGGGTGCCTGAAGGTTCTGTGAACTCCTTAAGCGTTTGAATGTAAAT1760.10868558372186371No Hit
CCTTCTGGGTCTGACCACAGTGGAATGAAACTGGACATCAGTAACAGCAAGA1750.10806805199617131No Hit
TACGAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1720.10621545681909407No Hit
AATGTGGGGGAACTTTCCCAACCAGCAGAACCATGAGCCAAATAAACCTCAT1720.10621545681909407No Hit
GTAGGGGGTACAGGAGGGAATCTATAGATTCAGTGCAATTCCTATTAAAATC1720.10621545681909407No Hit
ACTTGGGGGTCCGATCTTCGAATGGGAATAAAATAAAAGAATAAATAAAGAA1700.10498039336770927No Hit
CACGTGGTAGGTGAGCCACCGCTCCTGACCGAGAAAGGATTTCTATGCATTG1700.10498039336770927No Hit
AATCCAGGGGACTGCTCCGGGTGTCGGCTCCGGCGGGACGGGCGGTTCTGCG1690.10436286164201686No Hit
CTGTATGGGGGGGTATATTTGTAGGAGTGACATTGTTGGTTTTAGAGGTATA1680.10374532991632446No Hit
TAAAACGGGGATATACTGCTTACAAGAAACCCACTTGGAATATAATAACCAA1680.10374532991632446No Hit
ATTTAAGGGGAAGAACCGCCAGCTAAACTTTGTAAAATTCTTGACAAGAGCC1670.10312779819063204No Hit
AATGTAGGGCCGATCTTACGTGGGGCCGATCTGCCGTGGGGATCTTTTAAGT1670.10312779819063204No Hit
TTAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1660.10251026646493965No Hit
AACCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1650.10189273473924722No Hit
TCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.10127520301355482No Hit
TCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1630.1006576712878624No Hit
AATGCGGGGGTCCATGTTTCTCACATTGCTGTATTTTAGAAAATAGGGGTTA1620.10004013956217No Hit
CTATAAGGGGAATGCAGATGCAGAAAATCTAAAGACACTAGCAAATCAGCTT1620.10004013956217No Hit
TAGAAGTGGGGTCTAATAGGAGAAATATCTTCCCGAGCTCAGAATTAAAAGT1620.10004013956217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)