FastQCFastQC Report
Sat 31 Dec 2022
SRR5845834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93506
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT8640.9240048766924047No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC6590.7047676084957115No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1910.20426496695399227No Hit
ATAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1630.17432036446859026No Hit
CGGATGGGGCGATCTGCGCCTGGGGGTGTGTCCACTGGTAGCAATGTCCAGG1480.15827861313712488No Hit
CACTTAGGGATGCGAGTCAGAAGAAAACATTTGAAATTAATCCCAGACACCC1460.15613971295959617No Hit
ACTTGGGCCAAATTATAAATTCAATTAAAATATTGAAAGATAAAGTCACTGA1430.15293136269330312No Hit
CTGATTGGGAATCAGAATGAGCTTTGTCATCCAGAGCCAGAACTGCTGCAGA1430.15293136269330312No Hit
AAGATAGGGACCATCTCTGAGGTCAAAATTCAAAGGCCCCTCCATCCTAATA1400.14972301242701003No Hit
TCTTATGGGATCTAAAGACACCACCAAAATCTATTAGAACTGATAAACTAAT1390.14865356233824567No Hit
TCCAACGGGCTAGTACAGAGAGGTGGGGAGCCTTTTTTGTAGCAAAGGCTGT1360.1454452120719526No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG1340.1433063118944239No Hit
TTTATAGGGCAAAAATGTTTGGAATGGTTTTGGAAAAAATTATTATTCCTGA1340.1433063118944239No Hit
CTGTTGGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1340.1433063118944239No Hit
ACAAAAGGGCTAACCACCCTAGTTGGATAAAACTTAAAATAGTAATACAAAA1340.1433063118944239No Hit
CGTTAAGGGACTTCACATAAAAAATAGGGTGTTAATACCACCTTTACTGTAC1310.14009796162813082No Hit
TCGACGGGATCAGATTTAATTCACCAGATATTAACAAATTTATTTCTATTAA1300.13902851153936646No Hit
TTTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.1379590614506021No Hit
CGCAATGGGGATCTTTTGACCTGTGGTGCAAAAGCAGAAAATACAGCTGGAT1280.13688961136183775No Hit
CACACCGGGCCATAGGAATAGAAGAAATCACCCAGAGAGGGCATACAGACTA1240.13261181100678032No Hit
CCACTAGGGTAGAGAAAATATTTGTAACAACTAAAAATAGGGGAAGATAGGC1230.13154236091801594No Hit
CATAAAGGGTTTCCTGATAAACTTTTTAGACTAGTGTTATTTTGCAGAGATA1220.13047291082925158No Hit
CTCATCAGGGGTTGACTTACTAATGTACTGTCACAGACATATGGGTAGTTTT1220.13047291082925158No Hit
AATTCCGGGAAGTGAGTAATTCAAATTTCAATGTCTCTTCTGAAGTAACTAT1220.13047291082925158No Hit
ATTAACGGGTAGTCTCAAACTCCTGGGCTCAAGCAGTCCTCCTGCCTCAGTC1220.13047291082925158No Hit
AGGCCAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1220.13047291082925158No Hit
ACGAATGGGATGTGTATCACTGCCTGACTAGAAACCCCACTTTTCTTTTCTA1210.12940346074048725No Hit
AAGATAGGGAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCC1210.12940346074048725No Hit
GATCCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1210.12940346074048725No Hit
TTACTGGGGAGTGACTCTGAGGAGAAGGAGCCGCCTGTGAGTCAGCCTGCAG1210.12940346074048725No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1210.12940346074048725No Hit
GCCCAGGACTATATGTCTTTAAGAAAATTAAAACTATGGAAAATTTGTCTAG1200.1283340106517229No Hit
ACATATGGGCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGT1200.1283340106517229No Hit
GGATTAGGGGGAAAAGTCTGGAAGCACCTCATGTGGAGCTCAACTCAACCAC1190.12726456056295854No Hit
TGCTACGGGGGGAATTTAAAATAATACATACACTGGTTGATAAAGGGAAGCT1180.12619511047419418No Hit
AACATTGGGGGATGAAGCTATTAAGATAACCAACAGGCGTGTAAATGCCATT1170.1251256603854298No Hit
CACAGTGGGAAGGAAGAAGAAATCAAATCAGATGTGTTCAGATAACTGATCT1170.1251256603854298No Hit
TCACGTGGGTAAGAAAGTATAGCACATAACTATGCTTTGGTTTTGCTCCAAG1170.1251256603854298No Hit
CCTTTGGGGTAAAAGTAAGAGCTCAGTACAAAAAAGGAATGAACTTCTATTG1170.1251256603854298No Hit
TACGCAGGGAACAAACCAAAACCCCATGAGATTTCCACATTGCATAATTTTG1170.1251256603854298No Hit
AACAAGGGATTCTACTTGGAGTTCATTGAGTGTCTTAGATATGTAGATTCAT1160.12405621029666544No Hit
GTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1160.12405621029666544No Hit
GTAACTGGGAGTTCAAAATATTCTGGCTATCATTTGATTATTTTCTGTGAAA1160.12405621029666544No Hit
ATTCCAGGGACAACTAGATTATTATACTAGTTATAAATGCTGGTATATTATA1160.12405621029666544No Hit
GCATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.1229867602079011No Hit
GCAGTAGGGATTCTCTCTGGAGGCAATATGGATGAGTTCTTACGGATATGGG1150.1229867602079011No Hit
CACAACGGGGTAGGTGTTGATTATATTCTTCAAAAATTGGGCTTTCATCATG1150.1229867602079011No Hit
TAATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1140.12191731011913674No Hit
CTCTTTGGGACAGCACCATGAAAGAACTCAAGGAAAATATATCAATGTAAGA1140.12191731011913674No Hit
GTACAAGGGTCTTTCAAAGAGAAATGTGTGATTTTCATTTACTTGCTGATAT1140.12191731011913674No Hit
CTTCTGGGGGCTCGCTGCAACCTCTGACTCCCTGGTTCAAACGATTCTCCTG1140.12191731011913674No Hit
GACTTTTGGGAGAGGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAG1140.12191731011913674No Hit
AACTGCGGGTCTCTCTTCCCAGTGAAAATAAATGAGGAAATGTTTTCCAAAG1140.12191731011913674No Hit
GGGTCTGGGTGGGTGTGTACGTGTGAGTGTGTGTTTGTATACGTCTGGCAAT1140.12191731011913674No Hit
GTCAGAGGGTAAATGAAAGAAGATAAAACACTTACCAATTCATTTTAATGAA1130.12084786003037239No Hit
CGCGTGGGGGTAGGCATTTATTTCATGATTGATATGTCACAGAAATCATGGT1130.12084786003037239No Hit
GATCGTGGGATTTGGATTATTTTCATTGTTTTGAAAAATAGATATTAAGCAT1130.12084786003037239No Hit
AAGTGTGGGGTGACGTAATGAAATTACAGATTCAAGTGGATGAAAAGGGGAA1130.12084786003037239No Hit
TTGCAAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.11977840994160803No Hit
CGCGGAAGGGAATTTGTGTGTGTCCAACAGTTGAATTGAATGTCTATAAGGT1120.11977840994160803No Hit
AGTCTTGGGGAAGTAACATGACTAAAAAGAAGCGGGAGAATCTGGGCGTCGC1120.11977840994160803No Hit
AATACAGGGATGTCCTGTTTGGAACGGAGTTATTCCTTTTTTCCTTGTACCT1120.11977840994160803No Hit
AAACGTGGGGTGAATGTTTCTTTTTCCTGTATTGAATAAAATAAGGCCATTT1110.11870895985284367No Hit
TAATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1110.11870895985284367No Hit
GGCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.11870895985284367No Hit
GCAGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACTGTGTTAGCCA1110.11870895985284367No Hit
TAATATGGGTCCGATCTTGCGGTTGGGCGTCACTTGGTTTTGTAACATCAGG1110.11870895985284367No Hit
ACTGATGGGCCTGGTGGTCCTGGGCGGCCCCGAGACCCCAACACATGCTTCT1110.11870895985284367No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCCTGTCTCTTATAC1110.11870895985284367No Hit
TGATATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.11870895985284367No Hit
TTAACGCGGGGATTTATCACTTTTTAATAGGGTGAGCTTGAGAGTTTTCTTT1110.11870895985284367No Hit
ATATTAGGGGTACCCCTTAATGAAGCTGCACACTGGTTTTTGGCTGTTGTTT1100.1176395097640793No Hit
ACGCCCGGACAGCTTATGTATTTAACAACTAAACTGTTTAACATCTCCCGTG1100.1176395097640793No Hit
AACATTGGGATGGCTGCCTTTTGAAATATTTGATGTGTTGCCTGGCAGGATA1100.1176395097640793No Hit
AGTATTGGGAAGGAGGGGTAAAGACCTAGCTGCGTCTAGCATTTAAACGGAA1090.11657005967531495No Hit
CATCATGGGTACCATGTAAAATTTGTCTGATATTTGATTTGTGATACAATTT1090.11657005967531495No Hit
CGCTAAGGGGGAATGGAATGCTCAGACTCCTGCTGAGGGAGACACCAGCAAG1090.11657005967531495No Hit
CGCTAAGGGATATCAAATGAATATAGAACTAATATTGTCGGGAACACCTAAT1090.11657005967531495No Hit
GATTATGGGCTGGGATGAGAGTTTTGGATGCAGTGCTCGCTGTTGCTGAATA1080.11550060958655059No Hit
CGCTGCGGGAAATATGATAATTAAATAAAGGTTGAAACTAAAGTGATAGAAA1080.11550060958655059No Hit
TGTGATGGGGGGATTCTTTCCTAAAAAGGTTGATTACATTTCTTGCCTGTCC1080.11550060958655059No Hit
CGAACTGGGACTTCCTAAAATAAAACTAAGGTATCATCCTTACCCTTCTCTT1080.11550060958655059No Hit
TGCAGTTGGGCCTGGCAGCTGAACTCGGGTAGTCCAGTGGCCTAGCTGGTAC1070.11443115949778623No Hit
TCAATGGGGTCTTAACCTTAATGTGCTCATTCAAAGGACATGAACAGAAAAG1070.11443115949778623No Hit
ACCAAAGGGCCAAGGCTGACATTCCCAGCCCAAGAAAAGAGTTTAGTGCATG1060.11336170940902189No Hit
TTTTGAGGGAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT1060.11336170940902189No Hit
CCTCGCGGGGCTATGAAAAGGAAAAAGAGATTACATATTTTATCTTTGATCT1060.11336170940902189No Hit
AGCTTAGGGACCTCTTAATTTCTCAGCGTCCCACCAAGGAAATAGCTTTCAA1060.11336170940902189No Hit
AAATTCGGGGCTGCGTCTGTTCGTCAGCTGAGTTCCTTGTGAATCTCTGTTT1060.11336170940902189No Hit
ACTTTAGGGGGTTTTAAAGGATTTTATGTGTGTTTCCGAATGTTTTAGTTAG1050.11229225932025753No Hit
CGCTTTGGGATGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTG1050.11229225932025753No Hit
TAACCAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1040.11122280923149316No Hit
ATCCACGGGAAGTGGGAATCATGAAGCCTGCTTCCCAGAGCTGCCTTAAGGA1040.11122280923149316No Hit
TACTTCGGGCAAAATATAAATTAATGAATAAAATAAAATATTCAGAATGTCT1040.11122280923149316No Hit
TTAGGTGGGAATTTAAAATTAGCTTTTTTATTTTGAATTTCTGTACTTTGAT1040.11122280923149316No Hit
TTATTAGGGATTGACAATAATAACTATTAACAAAGCTTTTGATCTTATTCTG1040.11122280923149316No Hit
GTTCTGGGGTGCTCAGAACTGAATGTTTCTGCAGATCTTGTGGCATTTGTCT1040.11122280923149316No Hit
AGCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1040.11122280923149316No Hit
ACAACTGGGGTGACTTGAGGCAACTTTTCAGCTCTGAGCCCTATTTCCCATA1030.11015335914272881No Hit
CCCCGGGGTAACTAGTAATCCCATTGTTATACATGCTGACCCAAAATATAGC1030.11015335914272881No Hit
ACCGCTGGGAGGTGACTTCTAGCAGAGACCCTGGTTTTTTTCCTGTGCCCAC1030.11015335914272881No Hit
TAAGTCGGGAACATTCTTTTTAAAATTATTTTTATTTTTATTTTATTTTGAG1030.11015335914272881No Hit
TGGCATGGGGTCAGAAAGTATTCTCACAATTATGAGATGGATTATAATAAAA1030.11015335914272881No Hit
TATTCTGGGATCACTCAGAACTATTGATTCAAAGTTGGGTAGTGAATCAGGA1020.10908390905396445No Hit
TTAATTGGGAGCTCTGCCCAGGCCGGAGTCCTTTAGGGGAGGGATGGCAGCC1020.10908390905396445No Hit
GATGCCGGGATAAGTATGTGTTGTAATGTTATAGTGAGCTGTTATGTATCCC1020.10908390905396445No Hit
ACGTATGGGAAAGAATTCTCAGCAGAGCTCAAGATTGTAGAAACTCAGCAGA1020.10908390905396445No Hit
ACTACCGGGGACGCTCTTCCGATCTATATATGGGATTCATGTAACTGACATA1010.1080144589652001No Hit
ATTGTAGGGATTCCCTTCTGTTCCAGTCCAAAATAATTAGTAAATGGTTTTG1010.1080144589652001No Hit
ACTATAGGGGCGCCTGGCATCTGCTGTTTCTTTCAGAAGCTGGGCTGGGATG1010.1080144589652001No Hit
AGGCGTGGGGATTGATTTTTATATGTTGGCTTTATATCCTGAGACCCTTGTT1010.1080144589652001No Hit
TGTTTTGGGATGATCGAACTGAAGGATCTCTGAAAGGCCACAGCTGGAGCAA1000.10694500887643574No Hit
GGCACTGGGGATTCTCATACATGGCTATAATAGAAGGAGCAAAATGTATAAA1000.10694500887643574No Hit
GTTTCTGGGCTAGACGATGGTTTTAGATGATAACCACAGGTCTACAAGAGCG990.10587555878767138No Hit
AAGCTTGGGCACTATTGAGACCCACTTCTCAGAAAGCAAGATTCCTGTATTA990.10587555878767138No Hit
GTAGAGGGGGAGCCACGGCACCTGGCTTCTGTCTAACTTCTTTTATTTATCA990.10587555878767138No Hit
GCGCAGGGGCTGAGGAAGAAGAGAATTCTAAAAGCCTGGAAAACATATTTGG990.10587555878767138No Hit
TTACGGGCTAGAAAAAAAACTTCTTGAACTGAGGAGTTCAACATAGTTGCTG980.10480610869890701No Hit
TCTAGGGGTACATTTCTCATGCATTTTTTTCTATAATTGTAGTTATAAAAGT980.10480610869890701No Hit
TGAGATGGGATAGTCATGTGGGTCCAGATGGCCTCAGTCCTAGATGTTGGCA980.10480610869890701No Hit
TGTTGCGGGCATTTCAGGAACTGATATTTGACTGTTAACCTTTTTCCCATAC980.10480610869890701No Hit
GCTGCGGGGGGGCTGTGTGTACCTTGGTTGGTGACTCAGTGAGAAGAAGCAG970.10373665861014265No Hit
CAGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.1026672085213783No Hit
TGAGCAGGGATATTAGTCAGTTTTCACACTGCTGATAAAGACATACCTGAGG960.1026672085213783No Hit
TATGCAGGGACTGTGTTTTATTTCAAAACGTTGTTTTAAGTGATAGTGTCCT960.1026672085213783No Hit
ATACGAGGGTAAAACCAAGATTATCGTTGATTTAAACACATGAAAAGCAAAA960.1026672085213783No Hit
AAAGCAGGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGC960.1026672085213783No Hit
AGTAAAGGGTCTGAGGGCCTGCTTGGCCTCCTGGGGGCAAAGCCCCTCTGCC960.1026672085213783No Hit
TTGTCCGGGCTATTCATGGTGGGAGATGAGTGTGCAGTGGAGGAAGCTGGGC960.1026672085213783No Hit
CAGCAAGGGTGGTGTGGAGTCTGTTAGAGTCCTGCATGCTAGCTCTTAACCA960.1026672085213783No Hit
GCTATAGGGGAGTAAGCAATTCCTTGGATCTTATGCACAGAACTTGTACCAT960.1026672085213783No Hit
AAGTATGGGTAGCTACTGTAAAATTTACATGACTGTGAAATTCCATGCCTTT950.10159775843261395No Hit
GGATGGGGAGAAGCACCCCGGCAGTTGGCACTCACACAGCGGTCAGCAGCTG950.10159775843261395No Hit
ACAGTAGGGATTGAACAACTTGCCCAGGATTATACAGTGAGTAAGCACTTAA950.10159775843261395No Hit
GGGTTAGGGACCCTTGAACAACATAGGTTTTAACTGGGTGGCGACATTATAC950.10159775843261395No Hit
AATCTAGGGAATGCTGATAATACCTAAAGATGCATTGAATGCTTGGAAGAAT950.10159775843261395No Hit
TACTTGAGGGAAGTGACAACACCTTGATTTAAACTCCAGCATCACGCAATTT950.10159775843261395No Hit
GAATTGTGATTGGGTTCCCAGGCAGGGTTCAGAACATTGTGTTTATTGTCTG940.1005283083438496No Hit
TTTTCAGGGGAAACAAAATGAATTCTTATTATTGCTTTTTTAAAAAGTTTAT940.1005283083438496No Hit
GTTGCCGGGTCACATTGGGAATTAGGTTTTAACATATGAATTTTGAGGACAC940.1005283083438496No Hit
GCAATAGGGATGGCCTCCATACCTAATCTGTTGAGAGTTTTTTACCATGAAA940.1005283083438496No Hit
ATAGGTGGGGGAAAATTTTTTGCCTGTAGTTAGACCTCAGTAAATACTAAAT940.1005283083438496No Hit
GTCAAGGGGAATTCATAGCACAAAAAAAGTCATCTTAAATCTACTCTCAAAC940.1005283083438496No Hit
AATAGCGGGCAGAAAAGATGGTTGTTATTGGTGCAGGAGTAATAGGTGTAGA940.1005283083438496No Hit
ATTTTAGGGGTCAAGATGGACTTGGGTTTCCTCCATCATCTTTTCCCTTTTT940.1005283083438496No Hit
CATAAGGGGATGAAGATAGATTGGCAACCTACTCCTGAAATTCAATTTAAAT940.1005283083438496No Hit
CTGTTTGGGTCTGCTAAGAAAACTTTGTGCATGTTTTTTTGACTGGAAAGTG940.1005283083438496No Hit
GGCCACGGGTGGTATTCTGTCCTACAAGAGTCTCTAATCTTTCTAGACATGT940.1005283083438496No Hit
ATCTGCGGGGATAGTCTATCTGTGTTAGTATTTGGTGCTATCTATGTCCAGC940.1005283083438496No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)