FastQCFastQC Report
Sat 31 Dec 2022
SRR5845833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90615
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT8290.9148595707112508No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC6610.7294598024609612No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1930.21298901947801135No Hit
TTCCCTGGAGGAAATTCAGAAGAAATTAGAAGCTGCAGAAGAAAGACGCAAG1680.1853997682502897No Hit
ATAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1470.16222479721900346No Hit
CGGATGGGGCGATCTGCGCCTGGGGGTGTGTCCACTGGTAGCAATGTCCAGG1410.15560337692435028No Hit
AAGATAGGGACCATCTCTGAGGTCAAAATTCAAAGGCCCCTCCATCCTAATA1380.15229266677702366No Hit
TTTACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1360.15008552667880595No Hit
TCTTATGGGATCTAAAGACACCACCAAAATCTATTAGAACTGATAAACTAAT1340.14787838658058822No Hit
CTGATTGGGAATCAGAATGAGCTTTGTCATCCAGAGCCAGAACTGCTGCAGA1310.1445676764332616No Hit
TCAATGGGGTCTTAACCTTAATGTGCTCATTCAAAGGACATGAACAGAAAAG1310.1445676764332616No Hit
AGCAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.14346410638415272No Hit
TGTGATGGGGGGATTCTTTCCTAAAAAGGTTGATTACATTTCTTGCCTGTCC1300.14346410638415272No Hit
ACTTGGGCCAAATTATAAATTCAATTAAAATATTGAAAGATAAAGTCACTGA1280.14125696628593498No Hit
CACTTAGGGATGCGAGTCAGAAGAAAACATTTGAAATTAATCCCAGACACCC1280.14125696628593498No Hit
CGTTAAGGGACTTCACATAAAAAATAGGGTGTTAATACCACCTTTACTGTAC1280.14125696628593498No Hit
CGCAATGGGGATCTTTTGACCTGTGGTGCAAAAGCAGAAAATACAGCTGGAT1280.14125696628593498No Hit
TCACGTGGGTAAGAAAGTATAGCACATAACTATGCTTTGGTTTTGCTCCAAG1270.14015339623682616No Hit
TGCTACGGGGGGAATTTAAAATAATACATACACTGGTTGATAAAGGGAAGCT1270.14015339623682616No Hit
ACATATGGGCACCCAGAAGACTGTGGATGGCCCCTCCGGGAAACTGTGGCGT1250.1379462561386084No Hit
AAATTCGGGGCTGCGTCTGTTCGTCAGCTGAGTTCCTTGTGAATCTCTGTTT1210.13353197594217292No Hit
TGTTGCGGGCATTTCAGGAACTGATATTTGACTGTTAACCTTTTTCCCATAC1200.13242840589306407No Hit
CGCGGAAGGGAATTTGTGTGTGTCCAACAGTTGAATTGAATGTCTATAAGGT1190.13132483584395518No Hit
CGCTTTGGGATGAAGGCTGAATCAAAGACATTTCATCCACCAATATCATGTG1190.13132483584395518No Hit
ACCGCTGGGAGGTGACTTCTAGCAGAGACCCTGGTTTTTTTCCTGTGCCCAC1180.13022126579484633No Hit
TTTATAGGGCAAAAATGTTTGGAATGGTTTTGGAAAAAATTATTATTCCTGA1180.13022126579484633No Hit
CTGTTGGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1180.13022126579484633No Hit
TCGACGGGATCAGATTTAATTCACCAGATATTAACAAATTTATTTCTATTAA1180.13022126579484633No Hit
TAACCAGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1170.12911769574573748No Hit
AGGCCAGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA1170.12911769574573748No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1170.12911769574573748No Hit
ACACTAGGGACTTGTTGTCTGTTTTTGTTTCTTTCCCTTCTTTTCACAGGTG1160.1280141256966286No Hit
ACGAATGGGATGTGTATCACTGCCTGACTAGAAACCCCACTTTTCTTTTCTA1150.12691055564751974No Hit
GCAGTAGGGATTCTCTCTGGAGGCAATATGGATGAGTTCTTACGGATATGGG1150.12691055564751974No Hit
CCACTAGGGTAGAGAAAATATTTGTAACAACTAAAAATAGGGGAAGATAGGC1150.12691055564751974No Hit
CACAGTGGGAAGGAAGAAGAAATCAAATCAGATGTGTTCAGATAACTGATCT1150.12691055564751974No Hit
AGCACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.12691055564751974No Hit
TACTTCGGGCAAAATATAAATTAATGAATAAAATAAAATATTCAGAATGTCT1140.12580698559841086No Hit
ATTCCAGGGACAACTAGATTATTATACTAGTTATAAATGCTGGTATATTATA1140.12580698559841086No Hit
AAACGTGGGGTGAATGTTTCTTTTTCCTGTATTGAATAAAATAAGGCCATTT1130.12470341554930199No Hit
GGCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1130.12470341554930199No Hit
AACAAGGGATTCTACTTGGAGTTCATTGAGTGTCTTAGATATGTAGATTCAT1130.12470341554930199No Hit
ATCCACGGGAAGTGGGAATCATGAAGCCTGCTTCCCAGAGCTGCCTTAAGGA1130.12470341554930199No Hit
TTATTAGGGATTGACAATAATAACTATTAACAAAGCTTTTGATCTTATTCTG1130.12470341554930199No Hit
CTGTTTGGGTCTGCTAAGAAAACTTTGTGCATGTTTTTTTGACTGGAAAGTG1130.12470341554930199No Hit
TCTAGGGGTACATTTCTCATGCATTTTTTTCTATAATTGTAGTTATAAAAGT1120.12359984550019312No Hit
GTTTCTGGGCTAGACGATGGTTTTAGATGATAACCACAGGTCTACAAGAGCG1120.12359984550019312No Hit
GGATTAGGGGGAAAAGTCTGGAAGCACCTCATGTGGAGCTCAACTCAACCAC1120.12359984550019312No Hit
GATCGTGGGATTTGGATTATTTTCATTGTTTTGAAAAATAGATATTAAGCAT1110.12249627545108425No Hit
AGAGGTGGGGCTGTTAATAACGGGGCTAGAATTTACACTTATCTTTCATTCC1100.1213927054019754No Hit
GTCAAGGGGAATTCATAGCACAAAAAAAGTCATCTTAAATCTACTCTCAAAC1100.1213927054019754No Hit
TACGCAGGGAACAAACCAAAACCCCATGAGATTTCCACATTGCATAATTTTG1100.1213927054019754No Hit
CAGTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.12028913535286653No Hit
GCCCAGGACTATATGTCTTTAAGAAAATTAAAACTATGGAAAATTTGTCTAG1090.12028913535286653No Hit
GTAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.12028913535286653No Hit
GATCCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.12028913535286653No Hit
AATTCCGGGAAGTGAGTAATTCAAATTTCAATGTCTCTTCTGAAGTAACTAT1090.12028913535286653No Hit
CACACCGGGCCATAGGAATAGAAGAAATCACCCAGAGAGGGCATACAGACTA1090.12028913535286653No Hit
CTCTTTGGGACAGCACCATGAAAGAACTCAAGGAAAATATATCAATGTAAGA1080.11918556530375767No Hit
ATACGAGGGTAAAACCAAGATTATCGTTGATTTAAACACATGAAAAGCAAAA1080.11918556530375767No Hit
ACGCCCGGACAGCTTATGTATTTAACAACTAAACTGTTTAACATCTCCCGTG1080.11918556530375767No Hit
TGCAGTTGGGCCTGGCAGCTGAACTCGGGTAGTCCAGTGGCCTAGCTGGTAC1080.11918556530375767No Hit
GATGCCGGGATAAGTATGTGTTGTAATGTTATAGTGAGCTGTTATGTATCCC1070.1180819952546488No Hit
CGTTGCAGGATAAATATTCCATGAACAATTGGAGAAAATATATATTTTTTGT1070.1180819952546488No Hit
TAGCTCGGGATGTCACAAGATCTGATGGCTTTATAAGGTGGAGTTTTCCTGC1070.1180819952546488No Hit
ACAAAAGGGCTAACCACCCTAGTTGGATAAAACTTAAAATAGTAATACAAAA1070.1180819952546488No Hit
CCAGCGGGGATTCATAAAACAAGTCTTTAGAGACCTACAAAGAGACTCAGGC1070.1180819952546488No Hit
TTACGGGCTAGAAAAAAAACTTCTTGAACTGAGGAGTTCAACATAGTTGCTG1060.11697842520553992No Hit
AAGATAGGGAGACGGTGAACTCCAATGGGACCAGCGACCGCACAACCTCCCC1060.11697842520553992No Hit
GGGTTAGGGACCCTTGAACAACATAGGTTTTAACTGGGTGGCGACATTATAC1060.11697842520553992No Hit
AGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACACCACCTTC1060.11697842520553992No Hit
TTGCAAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.11587485515643105No Hit
ATGCCGGGGCTCTCGGCTTTCGGCTCGGAGGAGGCCAAGGTGCAACTTCCTT1050.11587485515643105No Hit
ATTAACGGGTAGTCTCAAACTCCTGGGCTCAAGCAGTCCTCCTGCCTCAGTC1050.11587485515643105No Hit
TGATATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1050.11587485515643105No Hit
TTAACGCGGGGATTTATCACTTTTTAATAGGGTGAGCTTGAGAGTTTTCTTT1050.11587485515643105No Hit
TTTTACGGGAATGCTTCTGTTTCTTTGCTAAAAGCTATTCTAAGATGTGCTG1050.11587485515643105No Hit
TTCTTAGGGAAGAACTATTGTTTACTGGGAATAAACTAAGCTCTATGAAGAA1040.11477128510732218No Hit
TAATATGGGTCCGATCTTGCGGTTGGGCGTCACTTGGTTTTGTAACATCAGG1040.11477128510732218No Hit
GCTTTCGGGATCTACATTGTAAGAGAACACAGTGGAAGATCCTGTCCTGATT1040.11477128510732218No Hit
CATCATGGGTACCATGTAAAATTTGTCTGATATTTGATTTGTGATACAATTT1030.11366771505821331No Hit
ATAAACGGGGATCTTACATGGGGGAAACAGTTTCTTTCTGAAACAGAGAAAA1030.11366771505821331No Hit
TAATGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.11366771505821331No Hit
TAAGTCGGGAACATTCTTTTTAAAATTATTTTTATTTTTATTTTATTTTGAG1030.11366771505821331No Hit
AAAAAGGGGGGGCGGCGGCGGCGGCGGCGCGGGAGGAGCAGAGCTGGACGCG1030.11366771505821331No Hit
AACATTGGGATGGCTGCCTTTTGAAATATTTGATGTGTTGCCTGGCAGGATA1030.11366771505821331No Hit
ACTGATGGGCCTGGTGGTCCTGGGCGGCCCCGAGACCCCAACACATGCTTCT1030.11366771505821331No Hit
CCTTTGGGGTAAAAGTAAGAGCTCAGTACAAAAAAGGAATGAACTTCTATTG1030.11366771505821331No Hit
GTCAGAGGGTAAATGAAAGAAGATAAAACACTTACCAATTCATTTTAATGAA1020.11256414500910444No Hit
AACATTGGGGGATGAAGCTATTAAGATAACCAACAGGCGTGTAAATGCCATT1020.11256414500910444No Hit
AGGCGTGGGGATTGATTTTTATATGTTGGCTTTATATCCTGAGACCCTTGTT1020.11256414500910444No Hit
AGTCTTGGGGAAGTAACATGACTAAAAAGAAGCGGGAGAATCTGGGCGTCGC1020.11256414500910444No Hit
TTTTCAGGGGAAACAAAATGAATTCTTATTATTGCTTTTTTAAAAAGTTTAT1010.11146057495999559No Hit
TAACAAGGGAGAGTGGGACCCTGTCTCAAAAATAAATAAGAAGGCGGGATGC1000.11035700491088672No Hit
GGGTCTGGGTGGGTGTGTACGTGTGAGTGTGTGTTTGTATACGTCTGGCAAT1000.11035700491088672No Hit
TTGTCCGGGCTTCCGATCTATGCAGGGCATAATGTTCAAGTTGTTATCTGTA990.10925343486177785No Hit
CATAAAGGGTTTCCTGATAAACTTTTTAGACTAGTGTTATTTTGCAGAGATA990.10925343486177785No Hit
TTCTCCGGGATTTGCAGTTGTAAAAATGATTACCGAACTCCAGACCTATTTG990.10925343486177785No Hit
GCAATAGGGATGGCCTCCATACCTAATCTGTTGAGAGTTTTTTACCATGAAA990.10925343486177785No Hit
CAGCAAGGGTGGTGTGGAGTCTGTTAGAGTCCTGCATGCTAGCTCTTAACCA990.10925343486177785No Hit
AATACAGGGATGTCCTGTTTGGAACGGAGTTATTCCTTTTTTCCTTGTACCT990.10925343486177785No Hit
CATCATGGGATTGTCAAAGATTTAGAAAAACATCTGTCAGGAATAAAGAAGT980.10814986481266899No Hit
TTGCACGGGGAGAGAATCCATTTAACTCCTGAGCTCATTCATAACATCTGTG980.10814986481266899No Hit
TATGCAGGGACTGTGTTTTATTTCAAAACGTTGTTTTAAGTGATAGTGTCCT980.10814986481266899No Hit
ACCGCCGGGATTTTTAAATCTTTTAAGTCTCTGTAAATAACAGTGCTTTTGA980.10814986481266899No Hit
TTACTGGGGAGTGACTCTGAGGAGAAGGAGCCGCCTGTGAGTCAGCCTGCAG980.10814986481266899No Hit
CGAACTGGGACTTCCTAAAATAAAACTAAGGTATCATCCTTACCCTTCTCTT980.10814986481266899No Hit
GGCACTGGGGATTCTCATACATGGCTATAATAGAAGGAGCAAAATGTATAAA970.10704629476356012No Hit
CACAACGGGGTAGGTGTTGATTATATTCTTCAAAAATTGGGCTTTCATCATG970.10704629476356012No Hit
GCTAGTGGGCCTTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCAT970.10704629476356012No Hit
TATCCGGGGTACTTGAGCAAATGTATCCAAGAAAGCAAGCAAATGAAAAGAA970.10704629476356012No Hit
TTTATGGGGAGGACTAACTATTTATGTAAAATTCTAGATACAATGCATTTGT970.10704629476356012No Hit
ACTATGGGGATTTGAATTAGGTAGTGGTTTCTTAAGTATGACTCCAAAAGCA970.10704629476356012No Hit
TACTTGAGGGAAGTGACAACACCTTGATTTAAACTCCAGCATCACGCAATTT970.10704629476356012No Hit
GATTATGGGCTGGGATGAGAGTTTTGGATGCAGTGCTCGCTGTTGCTGAATA960.10594272471445125No Hit
GAATTGTGATTGGGTTCCCAGGCAGGGTTCAGAACATTGTGTTTATTGTCTG960.10594272471445125No Hit
CATAAAGGGATGTCTTACTATTTGCGAAAATATATTTTTCCAGCTACGATAT960.10594272471445125No Hit
CTTCTGGGGGCTCGCTGCAACCTCTGACTCCCTGGTTCAAACGATTCTCCTG960.10594272471445125No Hit
AACATAAGGCTCACAGCGGGGGAAGCAATGAGAGCTGAGAAATTTTCAAAAT950.10483915466534238No Hit
ATATTAGGGGTACCCCTTAATGAAGCTGCACACTGGTTTTTGGCTGTTGTTT950.10483915466534238No Hit
ACAAAGGGGCTGTGGGGAAAAAAAAGAATGAGAACGTTTTTTATTTTTTTAT950.10483915466534238No Hit
GCATTAGGGCAGTCAGATGAAAATGCTGAGTTCTGAGAGAGTTCCAATGAGG950.10483915466534238No Hit
TTGTATGGGCACTCTGTTGGCTGAAGGAGGTAACTCAAACCTCAGGGTTTGT940.10373558461623351No Hit
TAATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.10373558461623351No Hit
TAGTTGGGGTAAGGATGGGAGCAGGATCAAGCATAATTGAAAACCTACAGTC940.10373558461623351No Hit
GGTTACGGGCGATGATCAGTCACAGCAGTCCTATGATTATGGAGGAAGTGGA940.10373558461623351No Hit
GTAAATGGGAATACAAGCAATGTTAGAACAGTAGCTTTCAAAGTCTCTTAAA940.10373558461623351No Hit
CAGCCCGGATACTTCTGAAGAACAAATTAAAGAATATTTTGGAGCCTTTGGA940.10373558461623351No Hit
CAGGGGAAGCTGGGGAGAAGAATGTTTTTTTCACAATTGAATTGCTGCATTT940.10373558461623351No Hit
ATTTTAGGGGTCAAGATGGACTTGGGTTTCCTCCATCATCTTTTCCCTTTTT940.10373558461623351No Hit
ATTAGGGGGGGGACGCGCGAGGACGCCGTGGCGGGAGAAGCGTTTCCGGTGG930.10263201456712463No Hit
TGAGATGGGATAGTCATGTGGGTCCAGATGGCCTCAGTCCTAGATGTTGGCA930.10263201456712463No Hit
GATTTTGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG930.10263201456712463No Hit
ACTTAGGGGCAGACTTTTTTTTTTAAATAACGGAATTTTGCAAAAAAACAGG930.10263201456712463No Hit
AAGCTTGGGCACTATTGAGACCCACTTCTCAGAAAGCAAGATTCCTGTATTA930.10263201456712463No Hit
GCATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10152844451801579No Hit
CACGGAGGGATCAGTGACTGCTTAATACTCTCGTTTTCTTTAAGACCTAGCT920.10152844451801579No Hit
ACTATAGGGGCGCCTGGCATCTGCTGTTTCTTTCAGAAGCTGGGCTGGGATG920.10152844451801579No Hit
AATACAGGGGAGAAAATGTTTTATTTTCATTCTTTTTAGAAAAAAAAGTATT920.10152844451801579No Hit
CGAAATAGGGCTGAAAGTTTTGATTTGAATGTAAAACAGGAAATTGGAATTC920.10152844451801579No Hit
ACAGTAGGGATTGAACAACTTGCCCAGGATTATACAGTGAGTAAGCACTTAA920.10152844451801579No Hit
TTAATTGGGAGCTCTGCCCAGGCCGGAGTCCTTTAGGGGAGGGATGGCAGCC920.10152844451801579No Hit
GCAGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACTGTGTTAGCCA920.10152844451801579No Hit
ACTCTAGGGCGATCTGCAAGTGGGTAGGAGGCATTTGTAAACAGTTGAAAGG920.10152844451801579No Hit
GTAACTGGGAGTTCAAAATATTCTGGCTATCATTTGATTATTTTCTGTGAAA920.10152844451801579No Hit
AATTGAGGGATTTTAGAAACACCTGCGTGGTGGCAGTTGTTGACGTTCTTGT920.10152844451801579No Hit
CGTAAGGGGCTCCCGAGTAGCTGGTACTACAGGAATGTGCCACCACGCTCGG920.10152844451801579No Hit
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCCTGTCTCTTATAC920.10152844451801579No Hit
TGTTTTGGGATGATCGAACTGAAGGATCTCTGAAAGGCCACAGCTGGAGCAA910.10042487446890692No Hit
TATTCTGGGATCACTCAGAACTATTGATTCAAAGTTGGGTAGTGAATCAGGA910.10042487446890692No Hit
GTACAAGGGTCTTTCAAAGAGAAATGTGTGATTTTCATTTACTTGCTGATAT910.10042487446890692No Hit
TGTTGAGGTATCATCCTTGTGGTGATCATTGTTGTGTATGAGAAGAGGAAGA910.10042487446890692No Hit
CCGTAAGGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGG910.10042487446890692No Hit
GTTCGTGGGCACCACCATTGACCTTTGTAAGTTCCCTGATGACAGTTAACAA910.10042487446890692No Hit
TTCATGGGTGTCATACATGAAGTTGCTCCGAGCTGTCTTTATTCTCTTCTTG910.10042487446890692No Hit
TTAGATGGGCTAGGTGTTATTTATTAACGTATTATAAAATAATGTTTGATCC910.10042487446890692No Hit
AGCGTAGGGCAATTAAATCCATTTCATTTAAAAGTGGGTCATGAAGTTTTAA910.10042487446890692No Hit
GATTACGGGGAGATATGAGGTTCCATGATAGATATCATATGGTATTCCTTGT910.10042487446890692No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)