FastQCFastQC Report
Sat 31 Dec 2022
SRR5845827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140027
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGCCGTCT12970.9262499375120513No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTCATCTCGTATGC11190.7991315960493333No Hit
GTAATGGGGAAATTATCATCAGAGTGAACAGAAAACCTACAAAATGGGAGAA3370.2406678711962693No Hit
TTTCCAGGGCATGAAATTTTAGATGATTGACTTGGTTTTCATTTGTAGAAAC1900.1356881172916652No Hit
ACGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1650.11783441764802503No Hit
TCTATCGCTGGGATGGATATGTAGTAAAATTATGAAAATATGTATGAGGATA1550.11069293779056896No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTACCCTC1540.10997878980482335No Hit
GCTCATGGGATGGCTCCAAACACATAGTTTTTAAGTTTTTAAAACTTTTTTG1420.10140901397587608No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)