FastQCFastQC Report
Sat 31 Dec 2022
SRR5845796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106567
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC5940.5573958167162443No Hit
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT3860.36221344318597687No Hit
AGAGCAGGGAAGGAGGAAGACCAAAGAGGAGGAAAACCAGGAATGTGGTGTT1840.17266133043062112No Hit
GCTATTGGGACAGACACGTAAATGCTGAGAGATTTTGTCAACACTAGGCCTG1770.16609269285989098No Hit
CTAGGTGGGGGCTGAATGGATTTAAAAAACAAGACGCAGTGGTTTGTTGCCT1700.1595240552891608No Hit
CTCCGTGGGCATCCAGGTGTTTCTGTTGAAACTGCTAGTCATAAATTATGTG1490.13981814257697037No Hit
AAAAATGGGAAGATGAAAACATTGCTTTCTTTCTTTTGTATTAGTGCTAATA1460.13700301218951458No Hit
TCAACGGGTCATGGATATGAGGGTGGTTTATGATTAAGGGTTTATATAAATA1440.13512625859787739No Hit
GATCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1430.1341878818020588No Hit
TGCAAAGGGGGAGTGGGCGGTGGCGATTGGTGTTGGCGGTCTGGCTCAGCTG1420.13324950500624022No Hit
CGGCAAGGGTGAAGACAAATAAGTTTGAGCACTTGAGTAAAACTTCACAGGT1390.13043437461878443No Hit
GGTTACGGGATATGAAAATGTGTATTTATAAGCTTTTAATCAGATAAATCTT1380.12949599782296584No Hit
ATGCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.12761924423132864No Hit
TGGCATGGGGGCTTCGTGACCATGACCAACTATGACGAGGCGGCCATGGCCA1350.12668086743551005No Hit
AGGAAAGGGGGGTGGCTCACTACTGAACACATGTGTATGATACTAAAGACGG1340.12574249063969145No Hit
TATGCGTGGGATAATTTCTAGGCTTATCTTGTTTTGCAGTTTTCATGTTTTG1290.1210506066605985No Hit
ACTCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1290.1210506066605985No Hit
GCAGCGGGGAGAACAGATGGGCAGGTCCACAGCACTGCCAGATTCTGCACAA1280.1201122298647799No Hit
GCTAGCGGGACTCTATTTTACAGACAAGGAAACTGAGTGACAATCAAAGGGT1270.1191738530689613No Hit
TTGAAGGGGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTAT1260.11823547627314271No Hit
TCAGCTGGGAGGATATGGCCGAATATTTCTTCCCTTGAGGTTTCTAACCCAA1260.11823547627314271No Hit
TGCGCAGGGTGTAAAATTTGTTCATTCGTTGAGGTAATGGTGCTATGTTTTT1250.11729709947732411No Hit
ACCTTTGGGATACCTGATGATTAATTGTGAGTGTTTGCAGAGCCAAGCACTG1250.11729709947732411No Hit
TTACCCGGGAAAAGGACCGGGAAAGAAAATCAGAGAGTGATAAAGATGTAAA1250.11729709947732411No Hit
GGACCAGGGATGAGACTGGAGAGATACCACGAAAGAATCTGGATGTCATGAA1240.11635872268150552No Hit
CATCGAGGGACTGTTACTGTGTGCATTTTTGTGTCCATTGTTTAAATGTAAT1240.11635872268150552No Hit
GGCGTGGGGACTGGAGAGAAACCTTACAAATGTGATGAATGTGGCAAAGTCT1240.11635872268150552No Hit
AGCCTGGGCAATGAGAGCAAAACTCAGTCTCAAACAAACAAACAAAAATAAG1230.11542034588568693No Hit
CTCTCTGGGCTATAGGCAGTTGTCCAGATAATCCCTTGTGTTGTATGGGAAA1220.11448196908986834No Hit
CACACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1220.11448196908986834No Hit
GTCACAGGGAAGGAAGAATCTGTGAAGCTATTCCGAGGGGTGAAGAAGATGG1220.11448196908986834No Hit
GTCACCGGGGGTGGAAGTGTAAATTAGTTCAACCATTGTGGATACAGTGTGG1220.11448196908986834No Hit
TAATATGGGGCAAATGAAGGAAAAAAATCACACAGTCATCTCAAAAGATACA1210.11354359229404974No Hit
GCGAACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1200.11260521549823117No Hit
GCAATAGGGACCAGATGGAATAACTCGCATGGAAGCTGAAAAGCTTTTTGGG1200.11260521549823117No Hit
TGTGCTGGGCGTGGTACTACCCGTCCTATCCTCCCTCCTACCCTGGCACGTG1190.11166683870241258No Hit
AAGCAAGGGACGGACGCTCGTCTTCGCCCGCCATGGCCGAGAGCGACTGGGA1190.11166683870241258No Hit
GCGCACAGGGGGGGATTTCGGGGCACCGGGTCCTCACCTTCACGAAAAAGGC1190.11166683870241258No Hit
GTCACGGGAAGTGTATGAAGAGGTGGTTTTTTAAAACTTCAAGTTGGAATTT1180.11072846190659398No Hit
AAAGCGGGTCTAGAAAGGAGCTTCTGCTCCTTATTCCCAGGCAAAGCCACAA1180.11072846190659398No Hit
TTGATGGGGGAACAGACTTGAGAAGATACAGATGGCAAACATGCACATTAAA1180.11072846190659398No Hit
GTAGCAGGGCTCTGGGCTTATACTGAGGCTCATCTACCCTAAAGGCCATCTC1170.10979008511077538No Hit
AATGCAGGGGAAGTAGAATCTCATTGTGGTTTTTGATTTGCATTTCCCTGAT1170.10979008511077538No Hit
TGCCATGGGATTTTTCCCACGGTAATGGAATATAACTATTTATCAATTTATC1160.1088517083149568No Hit
AAAGGCGGGGTATAGGAATCAAGTAATTTGATGACTAATGTGGATTATATTT1160.1088517083149568No Hit
TCAGCCGGGATTCTGAATGTATTGGGGACCCCTCCTTAGGCCTCGTTAAGTC1160.1088517083149568No Hit
TAAACGGGAAGAGGGATAACCTGCACCCCTCCCTGCAAGGGGTTCAGAGCCC1160.1088517083149568No Hit
CTGTCAGGGCACTGGAAACAAGTAAAAATAAATGTTAGACTTCATCTTTGTC1160.1088517083149568No Hit
CCGCTGAGGGTCCATAAGTTCACTGGTTCTGCTAGTGTCTTCTGAACATTCT1150.1079133315191382No Hit
AACGCTGGGGGTAGGGCCGAGCGCGGAACGCGAGGGGCTGCTGGGGTGTTTG1150.1079133315191382No Hit
AGCCTTGGGCACCCTTCCTTTCTCCCCAACCTCAAAAGAAAACAAAAAATTA1150.1079133315191382No Hit
GCATTTGGGGCCTGCCTTATAAGAAGAGCTCCTGAAGGAAGCACTAAGCATG1150.1079133315191382No Hit
AACATAGGGTAAAATAAAAATAAAAATTAAAAGAAAATGTAGTGCATATGTA1140.10697495472331961No Hit
CACGGAGGGGTTTTTAAAAAAATGTAGACTTGCATTTTTACTGTGCTTATTT1140.10697495472331961No Hit
ATTCATGGGACCTGGTGGCCTCTGCCCCGTCTCATGAGGGGCCCGAGCAGAA1130.10603657792750101No Hit
AACTGTGGGGGATAAATTTTAGAGGCATTAACTTTAAATTATGTGTTTGTAT1130.10603657792750101No Hit
TACCTCGGGGCAGGTGGAACACCCTGAAACCTGGAATCATTCTATGAGAATA1130.10603657792750101No Hit
CGCCGAGGGTAAGACAATTGAGCTATTTCTAATTTTCGGCTGCTATGAATAA1130.10603657792750101No Hit
TCAAATGGGCTTTCTTCAAATTTTAAAATTAAGACCTGTCCTGTATTAGTGC1130.10603657792750101No Hit
TTAGCGGGGATGTCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTA1130.10603657792750101No Hit
CTCTGGGGAAGTTTATGTTTGGATTACATGCACGTGTGAGATTATTTGCAAT1120.10509820113168242No Hit
CTAGACGGGCTTCCTATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCAT1120.10509820113168242No Hit
CTGCTGACGGGCATTTGTATGAGCTTCCTCATCTATAAAATGAGCATAACAT1120.10509820113168242No Hit
CTTCCGGGGTCTTTGCATCCTTCAATGACACTTTTGTCCATGTCACTGATCT1120.10509820113168242No Hit
GGAGTTGGGCAAAGGTCATCAAGCAGTGTTCATTTATCCTGTCACATGTTTC1120.10509820113168242No Hit
CTATCAGGGACTGTATTCATGTATCAGAAGAGTCAGTATAGTAAATATGTCA1110.10415982433586382No Hit
TTTACAGGGGAGCCGTGGGGCCTCCCGCACCCGTGGACACGCGAGCCTCATG1110.10415982433586382No Hit
AGCATCGGGCCTCCAAAATGCTTTATAGACCTCAAGAAATTCTAACAACACT1110.10415982433586382No Hit
TCCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1110.10415982433586382No Hit
TGTAAAGGGTACTTATGAGGGGAAAGAAGCTCTGTGTATTTTGTATTAAACC1110.10415982433586382No Hit
TTACGTGGGATCCGGCCTGCTGTTTTAAAGGGACCACACTTTTGAATACCTA1110.10415982433586382No Hit
TTTGCAGGGTCCGCGCGCCTGCCAGGCCGGGGCCGGCGGCGGAACAGCTTGG1110.10415982433586382No Hit
TCATAAGGGCCCTCACCCCATGACAGGCCCTGGTGTGTGATGTTCCCCGCCC1100.10322144754004524No Hit
TCAGGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.10322144754004524No Hit
AAGCTAGGGAGTGCTGGGATTACCAGCGTGAGCCACTGGGCCCGGCCCCAAA1100.10322144754004524No Hit
GCTCTAGGGGGCGGCTCCGCTATAAAACCCAGGCCGGCAGGATCGCTGCACC1100.10322144754004524No Hit
GAATATGGGGCAGCCATGAGAAGGAAGGATTCACCACCTCCAGGAAAAGTGT1090.10228307074422664No Hit
GTATTTGGGGGGTGTTCAAAATATATCTTAATTCAAAAGGGCTGCTTGAAAA1090.10228307074422664No Hit
AGTAAGGGGGGCCTGTCATTTGTCCAAATCACACTACGTGGAAGCAGAGGAA1090.10228307074422664No Hit
ATCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.10228307074422664No Hit
TACAAAGGGGGTCTATAAACATACATTTTGAGTTATTTTGTATATGGTGTAA1080.10134469394840805No Hit
AATCCAGGGATTTGTTGAATTATTAGTTACCACTGTCATTTCTTCAGCTATG1080.10134469394840805No Hit
GGCTCGGGTAAATCTTCATAGATAATAAATTTGTTTATTTTGCAATTTACAT1080.10134469394840805No Hit
GTAATGGGGAAATGCTAAACTTGAATAAGATGGAATGGCTGAACATGTGGTT1080.10134469394840805No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1080.10134469394840805No Hit
GTACTAGGGATAATTTGTTCAGCAATCCAGCAAGACCAACTTTTTAAAAAAA1080.10134469394840805No Hit
TATGGAGGGTGTTCAACGTATGAATGAATACATGTTAAAAATGCACAGTCCT1080.10134469394840805No Hit
TAGGATAGGGGGGCTTGTGCCTTGGTTTCTCCTTTGATTGCTGGTAAATTCT1070.10040631715258945No Hit
GCACTTGGGACTATTTGGGATTCTAGAAAAGGCAAAGCTATAAAGACAATAA1070.10040631715258945No Hit
TGTCAAGGGCAGGAGTGAGCCACCACGCCCAGCTAATTTTGTATTTTTTGTA1070.10040631715258945No Hit
TTTCAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1070.10040631715258945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)