FastQCFastQC Report
Sat 31 Dec 2022
SRR5845780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78880
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC7991.0129310344827585No Hit
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7180.9102434077079108No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCC1220.1546653144016227No Hit
CTCCATGGGACGAATGGAAAGACGACAATTTTTCTTCAATGCTTGATGCACT1060.1343813387423935No Hit
TAACCTGGGACCCGCTCCCGGAGACGCCGCCTCGCGATCCCCGCGCGGGCGG1040.13184584178498987No Hit
AACAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.12804259634888437No Hit
AATTGTGGGACACTTCAAAAGGTCTGAGGAGAAAATATTAGAAACCACTGAT1000.12677484787018256No Hit
ATTTAGGGGACTGAAAAGTCTGTATACTTTATAATTGCAATTATATGACATT1000.12677484787018256No Hit
TGCATGGGGATAAAAAACAAAACTGAGAGCTCCGAGGGTGAGCCTTCCTGGC990.12550709939148072No Hit
AGCATGGGGGGATACAGTTCCGGTGGGAGAACGCGGCTGCGAGGTTTTCGGC990.12550709939148072No Hit
TAATATGGGGCCCGCCTCCAGGGCCCTGGCCACCCCTCCCAAGCTCCACACT950.12043610547667342No Hit
TGCTCAGGGCAACCCCATCAAAAAGTGGGCGAAGGATATGAATAGACACTTT940.1191683569979716No Hit
GACAGAGGGTCTGCCTTTACGTTGACAGTGTTGAAGCAGGGTGAATAACTAG940.1191683569979716No Hit
CCCAAAGGGGACAGAAGCAATTTTATTGTTAAGCTGATTTTTTCCATATATC940.1191683569979716No Hit
CATAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC930.11790060851926977No Hit
CTAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC920.11663286004056794No Hit
AAATAGGGGATACTTTAGCCATTTGCAATATTAGCAAAAGCTAAATTCCTAG920.11663286004056794No Hit
ACGTAGGGGGAAACACCCCTGTTCCTAGCTCATGAAGAAGAGTCAGGTGGCC910.11536511156186613No Hit
AAAATGGGGGAGAGGTGAGAAACAGCAAAGCCAGTGCCTACTGTCTGGACCA910.11536511156186613No Hit
AACTGGGCTGAATGCTTATGATGAACATCAAACACTCCTGAAAGAACAGGAT910.11536511156186613No Hit
ATCAAGGGGGCCATAAGAAACTGCTGAGTACTTGGCAAACCCTTTCATAGTT910.11536511156186613No Hit
TCTCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC900.1140973630831643No Hit
ACACCTGGGATGCAGAGTTACATCTTATTCGTGTATTTCTCTGAGTATTTAT890.11282961460446247No Hit
CAGCCACCATCAGTTTCCGCATCTACAAGTCTGTTTTACAAGCAGTGCAGAA890.11282961460446247No Hit
GAAATAGGAGCTGGAAATATAAAAGATTTGCAAACTTCTTTGTTTCTGTCTC880.11156186612576065No Hit
AACTGTGGGGGTCATTGTAAATTAGATAGATATATTGCGTGTTAGAAGATTA870.11029411764705882No Hit
TTTGCTGGGACCACAGATGCACATCACCACACCCGGCTTTTTTTTTTTTTTT870.11029411764705882No Hit
TTCGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG860.10902636916835699No Hit
GAGACGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC860.10902636916835699No Hit
TGCTTTTGGGGCATTTTGTGATGAATACCGATAACAGACCCATGACTGGTAG860.10902636916835699No Hit
GGCCAGGGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGT850.10775862068965517No Hit
AGTCTAGGGCCAGGTCCTCTTGTAAATCTTTGCTTCTGTACATGCTGTTGAA850.10775862068965517No Hit
TAATTAGGGGATGCAAAAGTATTTTAATCTGAGACAATCAAAGTACAAAAAT840.10649087221095335No Hit
GTGACTGGGACATACTTTGAGGATTGACTTTATATAAGGTGCCCTGTAGAAA840.10649087221095335No Hit
GCAAAGGGAATGTTAAAACATCTGATTAGTTAGTTTTATACTTGATGATAAA840.10649087221095335No Hit
TTCGTCGGGGACATATATGAAAACATTCATTATTACATTTCCTTGTGTGTTT840.10649087221095335No Hit
TCACATGGGGACATTTCTCCCAAGAAGATTCACGGTCAATAAACACATGAAA830.10522312373225153No Hit
TCTGAAGGGACAGCAAAATATTATATTTCTATCCTATATTTCATGGGCTTAC820.1039553752535497No Hit
AATCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.10268762677484787No Hit
ATACCTGGGTGAACTTAAGTCAGACTTAAAGATTTGAGAAATTATTTGTGTC810.10268762677484787No Hit
AATTCCGGGCCGCAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAG810.10268762677484787No Hit
GTTTACGGGCGAGAATTTCTCCTGCCCAGGCATCCTCCTATTTTTCATGAGT810.10268762677484787No Hit
TTTAATGGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTG800.10141987829614604No Hit
ACCGTAGGGAACCATCCCATGTGGTGACTGATAATTGGTACATGTTTTCTAA800.10141987829614604No Hit
GCACATGGGGAACACTTCTTACTCTAAGGATTCTGACAGGTGACACATTATT790.10015212981744422No Hit
TTTGTGGGGTCTTGCTAAGAATGTGTTTTATGTTTTTAAAGGATTGTAAACC790.10015212981744422No Hit
AAAATTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA790.10015212981744422No Hit
TGTTTTGGGATTGGTGGAGTTTTTCTATATTTAAAAATACATATATATTTCA790.10015212981744422No Hit
GCAGCCGGGGATTTAGAAAAAGCTCCCATTGAGGAGAAATTATCTCAAGACA790.10015212981744422No Hit
AAATACGGGTGCAGGATTCAGTAACATCTATTCTGTAAGTTTCAGTTGTGAT790.10015212981744422No Hit
TTAGCAGGGACACAGGGCCAGTAATAATCCATATTGCCCTGAGGAAAAGTGC790.10015212981744422No Hit
ACGTAAGGGAATTGTGAAGAATGTGGCAAAGGCTTTAGTATATTCTCAACCC790.10015212981744422No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)