FastQCFastQC Report
Sat 31 Dec 2022
SRR5845779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79501
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7530.9471578973849386No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC7240.9106803688003925No Hit
AATTGTGGGACACTTCAAAAGGTCTGAGGAGAAAATATTAGAAACCACTGAT1110.13962088527188338No Hit
GCAAAGGGAATGTTAAAACATCTGATTAGTTAGTTTTATACTTGATGATAAA1060.13333165620558232No Hit
TTCGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1050.1320738103923221No Hit
CCCAAAGGGGACAGAAGCAATTTTATTGTTAAGCTGATTTTTTCCATATATC1020.12830027295254148No Hit
TCTCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1010.12704242713928127No Hit
AGATACGGGGATTCAGATGATGACTTTGTACAGCCCCGGAAGAAACGCCTCA1000.12578458132602105No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCC990.12452673551276085No Hit
CGTTCAGGGCATGAAGCTGAGCCCAGAACTGGGAAGCTGATACCAGTGGAGT980.12326888969950063No Hit
TGCATGGGGATAAAAAACAAAACTGAGAGCTCCGAGGGTGAGCCTTCCTGGC960.12075319807298023No Hit
GACACTGGGAGGTTCTGATTTCTTGATGTTCTTGCCAACAGTTGTTATTTGT960.12075319807298023No Hit
CTTGATGGGCAGCGCGGGATGTATTCAAGTAATTATTTTTTAGATAATAACT950.11949535225972No Hit
ACCGTAGGGAACCATCCCATGTGGTGACTGATAATTGGTACATGTTTTCTAA940.1182375064464598No Hit
AATCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.11697966063319958No Hit
TAACCTGGGACCCGCTCCCGGAGACGCCGCCTCGCGATCCCCGCGCGGGCGG920.11572181481993937No Hit
GGCCAGGGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGT920.11572181481993937No Hit
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCCTGTCTCTTATAC920.11572181481993937No Hit
CTAAGTGGGCTCCGCGAGGGAAACTATACCATGCAAACAGAAAACAAAAATG920.11572181481993937No Hit
AAAATGGGGGAGAGGTGAGAAACAGCAAAGCCAGTGCCTACTGTCTGGACCA920.11572181481993937No Hit
GACCAAGGGAGCGAGAATCAGACAGAGGGCAAAAACCAATTCGCTTCTCCCC910.11446396900667917No Hit
CTATCAGCGGGACTCCATCTAAAATAAGACATGCCTGAAATTTGGCAAGAAG900.11320612319341895No Hit
GTGACTGGGACATACTTTGAGGATTGACTTTATATAAGGTGCCCTGTAGAAA900.11320612319341895No Hit
ATTTAGGGGACTGAAAAGTCTGTATACTTTATAATTGCAATTATATGACATT900.11320612319341895No Hit
AACAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA900.11320612319341895No Hit
GTTTAAGGGGAAGCCTTTGTGGAAACACTAGGCTGATAGGGTTGGAAGGGGA890.11194827738015875No Hit
GACGTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC880.11069043156689852No Hit
GAAATTGGGACTTGAAGTGAGAAGATGATCCACAACTTGAGAAGACATTTAT870.10943258575363832No Hit
CGCAGGGGAGATGTGATTCGACAAGTACACAACAGTTTCGCCAGACAGCAAA870.10943258575363832No Hit
CTTTAGGGGGACTAATTAGCTAAAGATAGTGACTCCGCTGATTGGTGGGGAT870.10943258575363832No Hit
ATTGAAGGGCCAATTTCAAGGTTACTATAAGTTGTATAGTACAAGTGTTTTT870.10943258575363832No Hit
AGTCTAGGGCCAGGTCCTCTTGTAAATCTTTGCTTCTGTACATGCTGTTGAA870.10943258575363832No Hit
CTGACAGGGGGCCCCACCAATGTGGCCCCCATCATCAACCGTGTGGCTGAGC870.10943258575363832No Hit
GACGCCGGGAATTACATTATTTATAAACACACACACTAGCATGTTTTTCAGA860.10817473994037811No Hit
CATAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC860.10817473994037811No Hit
GAGTATGGGGGCATGACCTGGCGGCGGCCATGGCGGTGGACAAAAGCAACCC860.10817473994037811No Hit
AACTGGGCTGAATGCTTATGATGAACATCAAACACTCCTGAAAGAACAGGAT850.1069168941271179No Hit
GAAATAGGAGCTGGAAATATAAAAGATTTGCAAACTTCTTTGTTTCTGTCTC850.1069168941271179No Hit
AAGCTGGGATGGGTTCAGTGGAAATGTTTGGAACTCTGAAGGATTTAGACAA850.1069168941271179No Hit
CGTAACGGGCTGTTGTTGATGTTGTTTCTGCCTTGTAAGTTTTTGTTTTTAT840.1056590483138577No Hit
ATTTCAGGTCCAGTAAATGGTTGTGGGGAGGAAAGAGGTGGAGCCTCCCCAG840.1056590483138577No Hit
CTGCATTAGGGGAAAAGAAATTATCTTAATTATGAAATATTGTTTAAAAATA840.1056590483138577No Hit
ATAAATGGGGAGGTGAGGGTTGGCGGGAGAAAAATTATGTTTATTTGGAAAG840.1056590483138577No Hit
CTCCATGGGACGAATGGAAAGACGACAATTTTTCTTCAATGCTTGATGCACT830.10440120250059746No Hit
GCACGAGGGCGTCAGGGCTTGCCCCAAAGAGTCTATGGACTGGTGGATGTAA830.10440120250059746No Hit
GTCGCTCGGGGAGATGTGGTGGTATACTTTGTTTGCCATTTGTTTAAAAGAT830.10440120250059746No Hit
TTTTGAGGGAATGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGC830.10440120250059746No Hit
CTGTACATGGGATGCCTAATGTAAAGAAAATGTATTACATTCTGTATGTACA830.10440120250059746No Hit
GTTCATGGGACCCATCCTTACCAGGGAATGTTGCCATCAATTCCAGTTGAAA830.10440120250059746No Hit
GTTTACGGGCGAGAATTTCTCCTGCCCAGGCATCCTCCTATTTTTCATGAGT830.10440120250059746No Hit
TATGGTTGGGCACCTGTCCTATAAAATTTTGAATTGTGTATGTGATCTACAT820.10314335668733726No Hit
GCCGCCGGGGAAGGTGAAGCACTCACCATAATTCCCTAAATTCATGTTAGAA820.10314335668733726No Hit
TCGTTGGGGACTCACTGTAACCTTAAACTCCTAAGCTCAAGTGATCTCCTGC810.10188551087407707No Hit
GCAAAGGGAGCGCTCATGTCCCAGAAACACATCTTCCACCTCAGCAGCCAGT810.10188551087407707No Hit
ACTATGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC810.10188551087407707No Hit
CGTAATGGGGTTCTTCCTCTAGCGAAGGCAAGCGGTGCTGCTGCAGAGACAT810.10188551087407707No Hit
ATACCTGGGTGAACTTAAGTCAGACTTAAAGATTTGAGAAATTATTTGTGTC800.10062766506081684No Hit
ACCCAAGGTCAAGTACTGATTTTGTATTTCCTTCATTTTAAAAAATTGCCAA800.10062766506081684No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)