FastQCFastQC Report
Sat 31 Dec 2022
SRR5845778.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845778.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78413
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7470.9526481578309719No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC7460.9513728590922423No Hit
GCAAAGGGAATGTTAAAACATCTGATTAGTTAGTTTTATACTTGATGATAAA1200.15303584864754569No Hit
GTGACTGGGACATACTTTGAGGATTGACTTTATATAAGGTGCCCTGTAGAAA1070.13645696504406157No Hit
TAACCTGGGACCCGCTCCCGGAGACGCCGCCTCGCGATCCCCGCGCGGGCGG1010.12880517261168428No Hit
TGCATGGGGATAAAAAACAAAACTGAGAGCTCCGAGGGTGAGCCTTCCTGGC950.12115338017930699No Hit
GTTTAAGGGGAAGCCTTTGTGGAAACACTAGGCTGATAGGGTTGGAAGGGGA950.12115338017930699No Hit
AAAATGGGGGAGAGGTGAGAAACAGCAAAGCCAGTGCCTACTGTCTGGACCA940.11987808144057746No Hit
CGCAACGGGTGGAATGATAATGAATAATTGATGGCTAGATTGTTAATTCCAC930.11860278270184792No Hit
CTAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.11605218522438882No Hit
AATTCCGGGCCGCAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAG900.11477688648565926No Hit
ATAAATGGGGAGGTGAGGGTTGGCGGGAGAAAAATTATGTTTATTTGGAAAG900.11477688648565926No Hit
CTCCATGGGACGAATGGAAAGACGACAATTTTTCTTCAATGCTTGATGCACT890.11350158774692971No Hit
CTATCAGCGGGACTCCATCTAAAATAAGACATGCCTGAAATTTGGCAAGAAG890.11350158774692971No Hit
TCTCAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.11350158774692971No Hit
ATTTAGGGGACTGAAAAGTCTGTATACTTTATAATTGCAATTATATGACATT880.11222628900820017No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCC880.11222628900820017No Hit
CATGTTGGGCAAACCAAAGTTAACAGGGAGAGGATGGGGGAGGGGACAAATT860.10967569153074108No Hit
AACTGGGCTGAATGCTTATGATGAACATCAAACACTCCTGAAAGAACAGGAT860.10967569153074108No Hit
AATTGTGGGACACTTCAAAAGGTCTGAGGAGAAAATATTAGAAACCACTGAT850.10840039279201154No Hit
TAATATGGGGCCCGCCTCCAGGGCCCTGGCCACCCCTCCCAAGCTCCACACT850.10840039279201154No Hit
TTTGTGGGGTCTTGCTAAGAATGTGTTTTATGTTTTTAAAGGATTGTAAACC840.10712509405328198No Hit
GACCAAGGGAGCGAGAATCAGACAGAGGGCAAAAACCAATTCGCTTCTCCCC840.10712509405328198No Hit
AATCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA830.10584979531455244No Hit
TTTGAGGGACAATAATGATTTGCATGATACCTTGAAATTGGGGGGAGGGGGC830.10584979531455244No Hit
CTAAGTGGGCTCCGCGAGGGAAACTATACCATGCAAACAGAAAACAAAAATG830.10584979531455244No Hit
CTACTGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA830.10584979531455244No Hit
AACAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA830.10584979531455244No Hit
TCACATGGGGACATTTCTCCCAAGAAGATTCACGGTCAATAAACACATGAAA820.10457449657582289No Hit
GACGCCGGGAATTACATTATTTATAAACACACACACTAGCATGTTTTTCAGA820.10457449657582289No Hit
TCGAGCGGGTGAGGACTGGGAGGGCCGTTTTCTTCATAATTCCTACCCAGTT820.10457449657582289No Hit
AGATACGGGGATTCAGATGATGACTTTGTACAGCCCCGGAAGAAACGCCTCA820.10457449657582289No Hit
CTGCATTAGGGGAAAAGAAATTATCTTAATTATGAAATATTGTTTAAAAATA820.10457449657582289No Hit
TAAATCAGGGCGGATCCTCTGGTTGGAAGCATAGCCACTCAGTATTTGACCA810.10329919783709333No Hit
CTATGAGGGGTGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.10329919783709333No Hit
TCATGGGCAAGGCGCTGCGGGCCCAGGTGTGCTGGGCCCCTCTCAGGGGCAC810.10329919783709333No Hit
ATTTGTGGGGTACGAGCGTAGGACGATGCTTCTCTTCTGTCAGCCTGCAACT810.10329919783709333No Hit
ACGTAAGGGAATTGTGAAGAATGTGGCAAAGGCTTTAGTATATTCTCAACCC810.10329919783709333No Hit
CTTGATGGGCAGCGCGGGATGTATTCAAGTAATTATTTTTTAGATAATAACT800.10202389909836379No Hit
TGTTTTGGGATTGGTGGAGTTTTTCTATATTTAAAAATACATATATATTTCA800.10202389909836379No Hit
CATAAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC800.10202389909836379No Hit
TTAAGCCGGGAGAGTTGACTGACTACTTCAATATGCATGTTTTTATTAAAAA800.10202389909836379No Hit
AAAGAGGGGTAATCTGTGGCATGATAAATTAGCACTGTAATGCTAGCCTGTT790.10074860035963423No Hit
CACCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.10074860035963423No Hit
CATAATGGGCTAAGTTAGAGATTTCCAGATCACAGAGGGGCTGGGCATTCTC790.10074860035963423No Hit
TCTTAGGGGACCCAAAGTGACCAAAATACCCAAACAATATCGAACAAAGGAA790.10074860035963423No Hit
TTAGCGGGGTTGGATAAAAATGAATATATATAATTATGGAGATTCCTAAATC790.10074860035963423No Hit
CGTGCGGGGCATCAGTATATCCATATTAAAACGAGATGACAGAAGCCAAAGT790.10074860035963423No Hit
GTATCTGGGCATATGACACTTGGTTATAACTCTTTAGTTGGATATAACATTA790.10074860035963423No Hit
CCCAAAGGGGACAGAAGCAATTTTATTGTTAAGCTGATTTTTTCCATATATC790.10074860035963423No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)