FastQCFastQC Report
Sat 31 Dec 2022
SRR5845777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168077
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT9160.5449883089298357No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC6450.38375268478137997No Hit
TCGCTGGGGGGTATATTCACGTTGTTGATCAACCGTAATTACCATCCATCTC2280.1356521118296971No Hit
GCGTTTGGGAGTTACAGATAATGTAATAAAGGTTTAACTTAAAAATTTTTTT2110.125537699982746No Hit
TGCACAGGGAGAATAGTGATTTTAAAAGTAAAAATAAAAATAAACCTTACTT2080.1237528037744605No Hit
TGTTACGGGGGACTGGGAAATGTCAGCCGCTGGACTGTAGACGGATTTTCCT2010.1195880459551277No Hit
CTGTGTGGGGAACCAAAGTAATGTTCTTATGTTATTTCTTCCCTTCTTAAAA1950.11601825353855673No Hit
AACTGGGGGGAATGATGTACTTTAAAGGAAAAGAGAAAACTGCATCACAGTC1890.11244846112198575No Hit
TTGTTGGGGATAGTTTCACTGCCCTAAAAATCCCCTGTGCTTTCTCTGTTCA1890.11244846112198575No Hit
AATTGAGGGGATCTGAAGCGGGGCATGATTCCATTTCTAGTATAGGATCCTT1870.11125853031646209No Hit
ATCTCTGGGAACAAGATTCATTGCATAAAAGTTTCAGATTATTTTGCAGCAT1830.10887866870541478No Hit
CACTCACGGGATGCTTGACCCTTGCAGCCCTTTCTTTACCTCCTAATGCAAA1790.10649880709436746No Hit
TGAGGTGGGCTCATTACATGAATGTATTCTCTTTGTGTATTTTCTTAAAGGT1780.10590384169160563No Hit
ACATGTGGGAAGAAAATTGGATGCAAGACAATGGAGAAACTTTAAAACTAAA1780.10590384169160563No Hit
GCCTCAGGGTGGTAAAGATGATAATACGTAATCTTACTTTTGGGTAGCTGCT1780.10590384169160563No Hit
TATAGCGGATTGAGATTTAATAATATAAACTTGTTATTTGGGAATATAAGGT1760.10471391088608198No Hit
TGCAAGGATAGGAAGGCTGTTCAGTAAATCCATCCAAGAGATAACATTTCCT1750.10411894548332014No Hit
AAATTTGGGCTCTGCCACATGTCAGCAAATTTCTTTCCACCAGTCTTATAGC1740.10352398008055831No Hit
TCGACCGGGTATTCACATCACTCCAGAACCAGAATTTTCTTATGTTAGCTTT1740.10352398008055831No Hit
CGCTTAGGGAAGATGATGATGAAGAAGAAGAGGACAATGAATCTCGTTCTTG1730.10292901467779647No Hit
AAATGCGGGATAACATTTACAGGAAATGGAAAAGCTTCCATGTGTTACCCTG1730.10292901467779647No Hit
AGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGTTTTGTT1730.10292901467779647No Hit
TCGGTGGGGGGGAGACCGCGAGCGGCCCCACAGAGGACCAGGTGGAAATCCT1720.10233404927503466No Hit
CCCTGTTCTTGGTGACCCCAGTTGGGCTTTGGCCCCTCCAGCAACCTTGTGT1720.10233404927503466No Hit
TACTAGGGGCAATTCTGATTCTGAAATAGGATCAGGCTTTTACTACAACAGC1710.10173908387227282No Hit
CACAAAGGGAGAAGAGGCATGGAGATGTATATTTGTTTTTGTATTCTCTCCT1700.10114411846951099No Hit
TCTTTCGGGGCTTTTGTCTGATACTCAAATTTCTCTCTCAATCACTCAGATT1690.10054915306674918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)