FastQCFastQC Report
Sat 31 Dec 2022
SRR5845775.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845775.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164468
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT8360.5083055670403969No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC6360.3867013643991536No Hit
ATCTCTGGGAACAAGATTCATTGCATAAAAGTTTCAGATTATTTTGCAGCAT2190.1331566018921614No Hit
GCGTTTGGGAGTTACAGATAATGTAATAAAGGTTTAACTTAAAAATTTTTTT2160.13133253885254276No Hit
TCGCTGGGGGGTATATTCACGTTGTTGATCAACCGTAATTACCATCCATCTC1940.11795607656200598No Hit
AGCCAGGTTTACAAACTCAGACCCTTTTAAAAGTTGTTTAAATTGTTTTGTT1820.11065982440353139No Hit
TCGACCGGGTATTCACATCACTCCAGAACCAGAATTTTCTTATGTTAGCTTT1810.11005180339032516No Hit
TAACTAGGGATTACTTATTACCTCATTTACATTAACCCACTTTCTCACTGCA1780.10822774035070652No Hit
GGACACGGGCTAGGATTCTAGTTTGGGCTTTGTCTGGATTTGCTTTTCTCAG1780.10822774035070652No Hit
AAAGCTGGGAACATGGATAAATAAGGTTTTTTTTGTACCCAATTGATAGTTA1750.10640367731108787No Hit
TACTACGGGCTGCTCGGAACTATCAGTGTGACTGGGCAACTCACATCACCTT1740.10579565629788167No Hit
ACTTTCGGGGGGCCCAGGAAATATGGGCTGGAAATGCAAATATAATTGAAGT1730.10518763528467544No Hit
TACTAGGGGCAATTCTGATTCTGAAATAGGATCAGGCTTTTACTACAACAGC1720.10457961427146921No Hit
TTTAGTGGGAAAGAGACTAATTTCAGTTATACTTTTAAGCTTAGAATGTATG1720.10457961427146921No Hit
GTCACTGGGATTAGAAAATTGGCATATGTACTTTATTTTTGAAAAGGGAAGA1720.10457961427146921No Hit
ACAAATGGGCTGGAAGATATTAATTCTGTTTTGTAATCTACTAGATTTCAGT1710.10397159325826301No Hit
AATTGAGGGGATCTGAAGCGGGGCATGATTCCATTTCTAGTATAGGATCCTT1710.10397159325826301No Hit
TTGTTGGGGATAGTTTCACTGCCCTAAAAATCCCCTGTGCTTTCTCTGTTCA1710.10397159325826301No Hit
TATAGCGGATTGAGATTTAATAATATAAACTTGTTATTTGGGAATATAAGGT1680.10214753021864437No Hit
CCCTGTTCTTGGTGACCCCAGTTGGGCTTTGGCCCCTCCAGCAACCTTGTGT1670.10153950920543814No Hit
CTGTGTGGGGAACCAAAGTAATGTTCTTATGTTATTTCTTCCCTTCTTAAAA1670.10153950920543814No Hit
AAATTTGGGCTCTGCCACATGTCAGCAAATTTCTTTCCACCAGTCTTATAGC1660.10093148819223194No Hit
TGCAAGGATAGGAAGGCTGTTCAGTAAATCCATCCAAGAGATAACATTTCCT1660.10093148819223194No Hit
TGCACTGGGGACAAAAGACTAGCCTAAGACAAAATTGGAACTCGAGTAGGTT1650.10032346717902571No Hit
TTGTATGGGATCCATGCTGACTTTCATGATTTAGAAGTCTTTAACTGAATTA1650.10032346717902571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)