FastQCFastQC Report
Sat 31 Dec 2022
SRR5845771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180695
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7340.40620935831096594No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC6390.35363457760314343No Hit
TAGTGAAGGGGAGGGATATTATATAATGGTAAAAGGCCTTCTCCAACAGGAA2560.14167519853897453No Hit
CAACAAGGGGGAAGTGAAGGGAGGCGCAGTAGAAGTAGGAGATACTCTGGAT2320.12839314867594567No Hit
GAGGCTGAGGCGGGAGGATCGCTTGGGCCTGGGAGGCAGAGGTTGCAGTGAG2040.11289742383574532No Hit
CACACACGGGCAAGATTAATTTTCAGAGAGCTCTTACACAAGAATATTTGGT1990.11013033011428097No Hit
TGTGCTGAGGCCCATGTATTTACACTGAAAATGTTTTATCTTTTTAAAAACA1920.10625639890423089No Hit
TTTAGCGGGGTTGCTGTTTGTATTTCTTTCACAGTTTTGATAATATCACATC1900.10514956141564516No Hit
CATCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1890.10459614267135228No Hit
TTTGACGGGGATCTCAATAAAGTTGCAGGATGTAAAATAAAGATACAAAAAT1880.1040427239270594No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATA1880.1040427239270594No Hit
GACTTGAGGGAGGACCTACAAGGACAAAACCAAGTTTGCAGAAACACTAAAA1880.1040427239270594No Hit
TATTAAGGGTGATACCTGGGATACTGTGCCCAGAGCCTCGAGGAGGTAATGA1810.10016879271700933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)