FastQCFastQC Report
Sat 31 Dec 2022
SRR5845770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182285
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7400.40595770359601724No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC6200.3401267246345009No Hit
TAGTGAAGGGGAGGGATATTATATAATGGTAAAAGGCCTTCTCCAACAGGAA2620.14373097073264393No Hit
GAGGCTGAGGCGGGAGGATCGCTTGGGCCTGGGAGGCAGAGGTTGCAGTGAG2290.12562745151822696No Hit
GACTTGAGGGAGGACCTACAAGGACAAAACCAAGTTTGCAGAAACACTAAAA2260.12398167704418905No Hit
CAACAAGGGGGAAGTGAAGGGAGGCGCAGTAGAAGTAGGAGATACTCTGGAT2240.12288449406149711No Hit
TATTAAGGGTGATACCTGGGATACTGTGCCCAGAGCCTCGAGGAGGTAATGA1910.10478097484708013No Hit
TTTGACGGGGATCTCAATAAAGTTGCAGGATGTAAAATAAAGATACAAAAAT1890.10368379186438817No Hit
ATGCCTGGGTGAATCCTATCAGTAAAAACAAGCATTACAAAAGGGAACTCTG1870.10258660888169625No Hit
TGCAATGGGTAGCAGCGGCGCAGTTGCGCCTGTTTCGATAGGCGAGCGGATT1840.10094083440765834No Hit
GAAGCCGGGGGAGCATCTCCTGTTGCATTTCTTGAGGCATAAACCTCAGACT1840.10094083440765834No Hit
GCTAATGGGTAACTGAAAACATTAACATTCAGACACACTCCCTTCTGCCTTC1830.10039224291631237No Hit
AACTCGGGGTGGCCATGGCGGCGCCTTGGAGGCGATGGCCCACGGGGCTGCT1830.10039224291631237No Hit
TTTAGCGGGGTTGCTGTTTGTATTTCTTTCACAGTTTTGATAATATCACATC1830.10039224291631237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)