FastQCFastQC Report
Sat 31 Dec 2022
SRR5845769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178532
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGCCGTCT7140.39992830416956066No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACAATCCCATCTCGTATGC6360.35623865749557504No Hit
TAGTGAAGGGGAGGGATATTATATAATGGTAAAAGGCCTTCTCCAACAGGAA2310.12938856899603432No Hit
CAACAAGGGGGAAGTGAAGGGAGGCGCAGTAGAAGTAGGAGATACTCTGGAT2300.12882844532072682No Hit
GAGGCTGAGGCGGGAGGATCGCTTGGGCCTGGGAGGCAGAGGTTGCAGTGAG2270.1271480742948043No Hit
GACTTGAGGGAGGACCTACAAGGACAAAACCAAGTTTGCAGAAACACTAAAA2070.11594560078865414No Hit
CACACACGGGCAAGATTAATTTTCAGAGAGCTCTTACACAAGAATATTTGGT1910.10698362198373401No Hit
TCCAGAGGGGAACAAAGATACAACATACCAGAATCTCTGGGAAACAGCTAAG1910.10698362198373401No Hit
ACATTAGGGCTGCACACAAAATAGGCACATGACAAGATATGTGACACAATAG1870.10474312728250398No Hit
TTTGACGGGGATCTCAATAAAGTTGCAGGATGTAAAATAAAGATACAAAAAT1820.10194250890596644No Hit
GCGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGATA1790.10026213788004391No Hit
GAGTAGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCAG1790.10026213788004391No Hit
GCCTCGGGGAGTTTGAGTACGCCTGGTGCCTGGTGCGGAGCAAGTACAATGA1790.10026213788004391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)