FastQCFastQC Report
Sat 31 Dec 2022
SRR5845738.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845738.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140463
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGCCGTCT12110.8621487509166115No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGC11360.8087539067227669No Hit
GTGCTGGGATCCTAGCACTACAGGTGCACACCACTGCGCCTGGCTTTTTTTT2090.14879363248684707No Hit
GTAGCCATAAAGTGTTCTTTTCTCTGCACCATTTCCAAGAGCTCATTTTCTT1750.12458796978563749No Hit
GCAGTTGGGAGGCCGGGGCCGGCGGCGGAACAGCTTGGGACCCGAAACGCCT1740.12387603852971957No Hit
TGTAAGGGGGAGAAGCAGACGGTGATGTGGTTAATGAAAAGAAGAGAACTCC1650.11746865722645822No Hit
TCTTGTGGGAGAATAAAGAAACCTAATTAAATAATAAAAGCCTAGAAAAGGA1570.11177320717911479No Hit
AGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGA1560.11106127592319685No Hit
TTGTACGGGGGTGATAGAAGTATTGCTAGAAGCCAGTCTTGAATTTTCTTGG1560.11106127592319685No Hit
AGCTTAGGGGTCTTTCCCATATAAAAGCCAACCCAGAAAATTGGAAGAAATG1540.109637413411361No Hit
CATTGTGGGTGGAAATCTTTTGTATGGACCCAAAAATGAGTGACTAATTACA1540.109637413411361No Hit
TTTTTGGGGACAGGAACACTGAATTTGCTTGGACTCTTCTGGGTTAGCTCTT1490.10607775713177135No Hit
AAACTCGGGAGGAGTTAGTTAGAAAGGTCTTATTGATTTTACTTCTACTTTT1420.10109423834034587No Hit
TGACAAGGGCTCCTCCTCTGCTTTTTACTTTTTCGGAGAGGGGTCACAATTT1420.10109423834034587No Hit
GGAGCTCCTCATGTACTCCCATGACAACATCATCTGTGGCATCACCTCTGTT1410.10038230708442793No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)