FastQCFastQC Report
Sat 31 Dec 2022
SRR5845723.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845723.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183790
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGC9250.5032918004243974No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGCCGTCT9070.4934980140377605No Hit
TGTCTGGGGCATTGATTTTTGAAGGGTTTTTGGTGTGTCTATCTCCTTCAGT2870.1561564829424887No Hit
CCTTCAGGGCAGTTTTTGCAGTACTGTGTGAAGGGCTCGTGTGTTCATCCTG2800.15234778823657436No Hit
AACGCTGGGATTGCAGGTGGAGTGGAAGCCAAAGTTTGAGTATGAAAGGTTG2600.14146580336253334No Hit
TTCAAGGGGAAGGAAGGTTAAGTCACTTGCCAGAGGCCAGTAAGTGAGTATG2480.1349366124381087No Hit
TGCCAGGGTGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT2480.1349366124381087No Hit
CCAGTAGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC2000.10881984874041026No Hit
CAGACTGGGTACAAAAGGGCTTTAAAGTCAAAGTAATCCTGGGATTTTGGAG1980.10773165025300614No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCC1880.10229065781598565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)