FastQCFastQC Report
Sat 31 Dec 2022
SRR5845722.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845722.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184263
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGC8600.46672419313698355No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGCCGTCT8520.46238257273570926No Hit
AACGCTGGGATTGCAGGTGGAGTGGAAGCCAAAGTTTGAGTATGAAAGGTTG3260.17692103135192633No Hit
TGTCTGGGGCATTGATTTTTGAAGGGTTTTTGGTGTGTCTATCTCCTTCAGT2700.14652968854300646No Hit
CCTTCAGGGCAGTTTTTGCAGTACTGTGTGAAGGGCTCGTGTGTTCATCCTG2680.1454442834426879No Hit
TTCAAGGGGAAGGAAGGTTAAGTCACTTGCCAGAGGCCAGTAAGTGAGTATG2420.13133401713854653No Hit
TGCCAGGGTGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT2330.126449694187113No Hit
CCAGTAGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC2170.11776645338456444No Hit
TATTCAGGGGGCAGGCGCTTAGGGGCTGAGGCGCGATGGCAGGTGTCGGGGC2030.11016861768233449No Hit
TCAAACGGGAGAAGCACAATGTTTGAATAGTAGCTGATTATAAACTGCATTT1980.10745510493153808No Hit
ATAAGTGGGACCTGATAAATATGTATCTAGTTCTACAAGGTCACATTTATGT1970.10691240238137878No Hit
ATACCTGGGAGGCAGGCATTCTTAGCTGAGACACCAAGAGGTGGTTAAAGCC1900.10311348453026381No Hit
AACGACGGGCAGTACTTAAAATCCTGTTTGGTAAATGAACTAATACACTTTG1900.10311348453026381No Hit
CAGACTGGGTACAAAAGGGCTTTAAAGTCAAAGTAATCCTGGGATTTTGGAG1860.10094267432962667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)