FastQCFastQC Report
Sat 31 Dec 2022
SRR5845721.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845721.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180678
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGC8810.4876077884413155No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGCCGTCT8320.46048771848260445No Hit
TGTCTGGGGCATTGATTTTTGAAGGGTTTTTGGTGTGTCTATCTCCTTCAGT3130.17323636524646055No Hit
AACGCTGGGATTGCAGGTGGAGTGGAAGCCAAAGTTTGAGTATGAAAGGTTG2750.15220447425807238No Hit
TGCCAGGGTGATACTGTTTTAAATAATCTGTAATTTCAATTTTTTTTTTTTT2710.14999059099613676No Hit
TTCAAGGGGAAGGAAGGTTAAGTCACTTGCCAGAGGCCAGTAAGTGAGTATG2480.13726076224000708No Hit
CCTTCAGGGCAGTTTTTGCAGTACTGTGTGAAGGGCTCGTGTGTTCATCCTG2410.13338646653161979No Hit
AACGACGGGCAGTACTTAAAATCCTGTTTGGTAAATGAACTAATACACTTTG1950.10792680901936041No Hit
CAGACTGGGTACAAAAGGGCTTTAAAGTCAAAGTAATCCTGGGATTTTGGAG1880.10405251331097312No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCC1840.10183863004903752No Hit
CCAGTAGGGGGCGGCCGGCCAGTTCCAGCCCGCACCCCGCGTCGGTGCCCGC1820.1007316884180697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)