FastQCFastQC Report
Sat 31 Dec 2022
SRR5845693.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845693.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127415
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGCCGTCT13461.0563905348663816No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCCATCTCGTATGC12340.968488796452537No Hit
CAAAAAGAGGCTTATGCAACACACATGTGAGCAGGGCACGGTGGGAGACTGC2570.20170309618176824No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTCAATCC2030.15932190087509318No Hit
TCATTGGGCGCAAAGGGAAACTTATCTTCTTTTATGAATGGAGCGTCAAACT1950.1530432052741043No Hit
TATGCTGGGATGTATGACCTGGATGGTGATGGCAAGATCACCCGAGTGGAGA1770.13891614017187928No Hit
GCTATAGGGGCCAGCCAGTCCGCCCGTCCGGAGCCCGGCTCGCTGGGGCAGC1720.13499195542126124No Hit
GGCACTGGAAGAATGGACAGCATAAAAACTGGACAGCACTACAAACATGAAA1680.13185260762076678No Hit
GCAATAGGGAAGACTCTGTCTCAAAAATAAAATAAAATGAAATACATTAGCA1610.1263587489699015No Hit
AGATAGGGGTTAGCAGTTGGTTGAATCTGTGGATGCAGAATCCATGGGTACT1590.12478907506965428No Hit
GCACTGGGGGTCCTGACCAAACCGTCTTTGAAAGGAATACACATGTGGAGGG1580.12400423811953067No Hit
TCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1560.12243456421928345No Hit
GAATGGGGCTGCCTCAGTTTTCTCACAACCTACTTTCTCCTCAAATACACAA1530.1200800533689126No Hit
GCTCTCGGGGCGGCGTCGCCAGGAGGAGCGCGCGGGCACAGGGTGCCGCTGA1520.119295216418789No Hit
TCTAAAGGGATTTTCGGTCATATAAAAACTAACGAATTTTGCATTCCACAGA1520.119295216418789No Hit
TGGTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1490.11694070556841815No Hit
GTCTGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1470.11537103166817093No Hit
ATGTCCGGGTGAAATTTGGAATTTAGTATTTATGAATGAATATGTTCCAGAT1470.11537103166817093No Hit
CATGCGGGGAGTCCTCAGCGGGGCGCGTGGCGAGCGGACTCGACTCGGCACC1470.11537103166817093No Hit
CACTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1460.11458619471804733No Hit
ACCAGGGGCAGTGAAAGTACAAGATAAGCCTTGTACTTGTCAAGGCTTATTT1460.11458619471804733No Hit
GATAGTGGGATGAAAATAATTGCTAGTTCATTTTCCCAAGATTCTCCACCAA1440.11301652081780009No Hit
TAAGCAGGGGCAGATTTCTCTAGCTTAATTCTTATCACTTTCCTAGATGAAC1420.11144684691755287No Hit
GACATTGGGATGAGATTATGGGCTGGCTCCATTTCTTGGACTTAAAATGCAT1420.11144684691755287No Hit
ACACCTGGGGAAATCAGATTGCCAACTTCTTGACTTTCAATGTTAGACATTT1400.10987717301730565No Hit
ACAGGTGGGTTATAATAGTATTTCTGGAATTTAAATCATTAGTATATTAAAT1390.10909233606718205No Hit
TTAGCAGGGTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCT1390.10909233606718205No Hit
GCAGTGGGGCTGCAGAGAACATAAAATGTGGGTGAATTTTTGGGAAGCCATG1380.10830749911705842No Hit
GCTCGGGGAGGGTGAACCATGAACACTTATTAGCTATTTGTCAACTTAGATT1380.10830749911705842No Hit
TTCTAAGGGCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGC1380.10830749911705842No Hit
GTCTTCGGGTTGATGACCTCGATATGGATTTAGATTATCAAATGTGTTTGGT1380.10830749911705842No Hit
GTACTCGGGTGTTGCCCAGGAGGGTCTCGAACTCCTGGCCTCAAGTGATCCA1370.10752266216693482No Hit
CATGGGAGAAATCCCAGCTTTAAAGTAGGAAATGTAATAAGGACTTCATTTG1370.10752266216693482No Hit
TGCTGTGGGATGCAAAGTCTCAGAATATACAAGTACCACTGGGCTTGTACCA1370.10752266216693482No Hit
ACCTAAGGGTGGTTTTGTGATTGATTTAACTTGTTTGGATATGCTGTAGGGG1370.10752266216693482No Hit
TTAGCTGGGCATGAGTCTCGGCTGGTTGGCGCATGTGACCTTTGAAGGATGA1360.1067378252168112No Hit
GAACATGGGGTTAGAAAATATAATCATAAACTGAAACTAAGATTTAGAAATT1350.1059529882666876No Hit
TAATAAGGGTACAACAGATTGAATCTCGAAGTTGATTCAGGAGACAATTATT1350.1059529882666876No Hit
TCTAGTGGGTGGGAGATTGTCCATATCCCTCCGTAATTAAGGGCCAGCAAGT1330.10438331436644038No Hit
TAGCTTGGGAAGGCATTGAGATGGAGCTCACATAAACACATTTGGATGGTGA1330.10438331436644038No Hit
TAGTGAGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTG1330.10438331436644038No Hit
AAACGAGGGAGAAACCTTGCCCCTGATTTTGGGGCTCATCTTGGGCCTCGTC1320.10359847741631675No Hit
CAGCATGGGCAGTTCATGTGGGAATCATGAACATATTTTAAGCAAATTTAGG1320.10359847741631675No Hit
GCACCAGGGACTTGAAATCAGTTTGGCAATATTTATCACAAGTCTTAGAATG1320.10359847741631675No Hit
TTTCCAGGGACAATCACCCATATGAAAAAATTGTTTTCACCTGATGAAAATG1320.10359847741631675No Hit
TAGAATCGGGATGAATGGAGATCAACTATACTTGGTCCACCGGGTTCTGTAT1310.10281364046619317No Hit
CTCGCTGGGAGTCCATTAAAAGTTTGTCACCTTACATATAAATAAATTATTC1310.10281364046619317No Hit
TTTGTAGGGACCAGACTCATGTTGTTCTTAGTTGCATGGCTCTAAGGTGATA1310.10281364046619317No Hit
CAACGTGGGGTTGGTAACTCTTAAAGTGGCAAAGTATTCTCAAATCAAATGA1310.10281364046619317No Hit
TAAGTGAGGGATTATACAACTTTGCAACTGAAGTATGTTTCCTTGTAGCAAA1310.10281364046619317No Hit
GTTGGTGGGATATTATAAATTACTTTAACGGTACACTTGTTTTTCATTGCTT1310.10281364046619317No Hit
ATTAGGGACCAACAAAGATCAAAAGAGACAAAGAAGGCCATTACATAATGGT1310.10281364046619317No Hit
ATGCCTGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1300.10202880351606954No Hit
TGCCGGGGGACCAGGTTGCACTTGGAAGAGATCCTCGCGTCTTCATGAGGCA1300.10202880351606954No Hit
ACATATGGGCTGGGACGGCGGCAGTATTGAAGCTCGTGACAAGTGCCTTCAC1290.10124396656594592No Hit
TTGCATGGGACACACAAAGACTAGAATGCAGAAATTAAAGAAGAAAAAAGAA1280.10045912961582232No Hit
ACGCGAGGGGACAACAGAGCATACGAGATGACCAAGGTAGCCCATCGAGTCT1280.10045912961582232No Hit
CACCGAGGGTGCTGGCGGGACTGAATTTTCTCAAGGCTTACAGGCCCTTCCA1280.10045912961582232No Hit
ATTTGAGGGGTGGCTGAAACTCTTAAATGTAAATTAATGCTGGACTTAACAG1280.10045912961582232No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)